Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4889 and PP_4890 are separated by 56 nucleotides PP_4890 and PP_4891 are separated by 301 nucleotides
PP_4889: PP_4889 - Adenylosuccinate synthetase, at 5,557,960 to 5,559,252
_4889
PP_4890: PP_4890 - ATP phosphoribosyltransferase regulatory subunit, at 5,559,309 to 5,560,496
_4890
PP_4891: PP_4891 - integral membrane ATP-dependent zinc metallopeptidase / HflK-HflC complex, at 5,560,798 to 5,561,667
_4891
Position (kb) 5559
5560
5561 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 5559.674 kb on - strand, within PP_4890 at 5559.674 kb on - strand, within PP_4890 at 5559.992 kb on - strand, within PP_4890 at 5560.103 kb on - strand, within PP_4890 at 5560.103 kb on - strand, within PP_4890 at 5560.103 kb on - strand, within PP_4890 at 5560.291 kb on - strand, within PP_4890 at 5560.349 kb on - strand, within PP_4890 at 5560.349 kb on - strand, within PP_4890 at 5560.450 kb on - strand at 5560.486 kb on - strand at 5560.498 kb on - strand at 5560.713 kb on - strand at 5560.713 kb on - strand at 5560.713 kb on - strand at 5560.713 kb on - strand at 5560.713 kb on - strand at 5560.752 kb on + strand at 5560.752 kb on + strand at 5560.886 kb on + strand, within PP_4891 at 5560.935 kb on - strand, within PP_4891 at 5560.935 kb on - strand, within PP_4891 at 5560.946 kb on + strand, within PP_4891 at 5560.946 kb on + strand, within PP_4891 at 5560.946 kb on + strand, within PP_4891 at 5560.946 kb on + strand, within PP_4891 at 5560.946 kb on + strand, within PP_4891 at 5560.947 kb on - strand, within PP_4891 at 5560.947 kb on - strand, within PP_4891 at 5560.947 kb on - strand, within PP_4891 at 5560.947 kb on - strand, within PP_4891 at 5560.950 kb on + strand, within PP_4891 at 5560.951 kb on - strand, within PP_4891 at 5560.951 kb on - strand, within PP_4891 at 5560.951 kb on - strand, within PP_4891 at 5561.000 kb on + strand, within PP_4891 at 5561.001 kb on - strand, within PP_4891 at 5561.278 kb on - strand, within PP_4891 at 5561.278 kb on - strand, within PP_4891 at 5561.366 kb on + strand, within PP_4891 at 5561.367 kb on - strand, within PP_4891 at 5561.367 kb on - strand, within PP_4891
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 5,559,674 - PP_4890 0.31 -4.2 5,559,674 - PP_4890 0.31 -3.1 5,559,992 - PP_4890 0.57 -1.0 5,560,103 - PP_4890 0.67 -2.4 5,560,103 - PP_4890 0.67 -1.6 5,560,103 - PP_4890 0.67 -3.2 5,560,291 - PP_4890 0.83 -1.9 5,560,349 - PP_4890 0.88 -2.4 5,560,349 - PP_4890 0.88 -3.7 5,560,450 - -3.0 5,560,486 - -2.0 5,560,498 - -1.3 5,560,713 - -0.5 5,560,713 - -1.6 5,560,713 - -2.0 5,560,713 - +0.2 5,560,713 - -0.7 5,560,752 + -0.7 5,560,752 + +0.1 5,560,886 + PP_4891 0.10 +2.7 5,560,935 - PP_4891 0.16 +1.9 5,560,935 - PP_4891 0.16 -0.1 5,560,946 + PP_4891 0.17 +2.7 5,560,946 + PP_4891 0.17 +0.7 5,560,946 + PP_4891 0.17 -0.1 5,560,946 + PP_4891 0.17 +1.7 5,560,946 + PP_4891 0.17 +0.7 5,560,947 - PP_4891 0.17 -1.2 5,560,947 - PP_4891 0.17 +1.1 5,560,947 - PP_4891 0.17 +0.7 5,560,947 - PP_4891 0.17 +1.7 5,560,950 + PP_4891 0.17 +1.1 5,560,951 - PP_4891 0.18 -0.7 5,560,951 - PP_4891 0.18 +0.3 5,560,951 - PP_4891 0.18 +4.3 5,561,000 + PP_4891 0.23 +2.7 5,561,001 - PP_4891 0.23 -1.3 5,561,278 - PP_4891 0.55 +0.3 5,561,278 - PP_4891 0.55 +0.1 5,561,366 + PP_4891 0.65 +2.7 5,561,367 - PP_4891 0.65 +1.1 5,561,367 - PP_4891 0.65 +1.1
Or see this region's nucleotide sequence