Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4746 and PP_4748 are separated by 716 nucleotides PP_4748 and PP_4749 are separated by 90 nucleotides PP_4749 and PP_4750 overlap by 4 nucleotides
PP_4746: PP_4746 - transposase, at 5,403,343 to 5,403,702
_4746
PP_4748: PP_4748 - Amino acid ABC transporter, periplasmic amino acid-binding protein, at 5,404,419 to 5,405,219
_4748
PP_4749: PP_4749 - Amino acid ABC transporter, permease protein, at 5,405,310 to 5,405,969
_4749
PP_4750: PP_4750 - Amino acid ABC transporter, permease protein, at 5,405,966 to 5,406,607
_4750
Position (kb) 5404
5405
5406 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5403.878 kb on + strand at 5403.879 kb on - strand at 5403.881 kb on + strand at 5404.144 kb on + strand at 5404.145 kb on - strand at 5404.178 kb on + strand at 5404.178 kb on + strand at 5404.179 kb on - strand at 5404.389 kb on + strand at 5404.521 kb on - strand, within PP_4748 at 5404.521 kb on - strand, within PP_4748 at 5404.564 kb on + strand, within PP_4748 at 5404.747 kb on + strand, within PP_4748 at 5404.760 kb on + strand, within PP_4748 at 5404.850 kb on - strand, within PP_4748 at 5404.885 kb on + strand, within PP_4748 at 5404.885 kb on + strand, within PP_4748 at 5404.885 kb on + strand, within PP_4748 at 5404.886 kb on - strand, within PP_4748 at 5404.933 kb on + strand, within PP_4748 at 5405.113 kb on + strand, within PP_4748 at 5405.113 kb on + strand, within PP_4748 at 5405.114 kb on - strand, within PP_4748 at 5405.114 kb on - strand, within PP_4748 at 5405.185 kb on + strand at 5405.269 kb on + strand at 5405.269 kb on + strand at 5405.269 kb on + strand at 5405.270 kb on - strand at 5405.270 kb on - strand at 5405.311 kb on + strand at 5405.311 kb on + strand at 5405.312 kb on - strand at 5405.383 kb on + strand, within PP_4749 at 5405.383 kb on + strand, within PP_4749 at 5405.384 kb on - strand, within PP_4749 at 5405.384 kb on - strand, within PP_4749 at 5405.384 kb on - strand, within PP_4749 at 5405.384 kb on - strand, within PP_4749 at 5405.384 kb on - strand, within PP_4749 at 5405.416 kb on + strand, within PP_4749 at 5405.417 kb on - strand, within PP_4749 at 5405.417 kb on - strand, within PP_4749 at 5405.417 kb on - strand, within PP_4749 at 5405.417 kb on - strand, within PP_4749 at 5405.417 kb on - strand, within PP_4749 at 5405.417 kb on - strand, within PP_4749 at 5405.665 kb on + strand, within PP_4749 at 5405.666 kb on - strand, within PP_4749 at 5405.752 kb on + strand, within PP_4749 at 5405.752 kb on + strand, within PP_4749 at 5405.753 kb on - strand, within PP_4749 at 5405.753 kb on - strand, within PP_4749 at 5405.753 kb on - strand, within PP_4749 at 5406.042 kb on + strand, within PP_4750 at 5406.042 kb on + strand, within PP_4750 at 5406.174 kb on + strand, within PP_4750 at 5406.174 kb on + strand, within PP_4750 at 5406.175 kb on - strand, within PP_4750 at 5406.175 kb on - strand, within PP_4750 at 5406.175 kb on - strand, within PP_4750 at 5406.200 kb on - strand, within PP_4750
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 5,403,878 + -1.9 5,403,879 - -0.9 5,403,881 + -0.6 5,404,144 + -0.1 5,404,145 - +1.0 5,404,178 + +0.7 5,404,178 + +0.2 5,404,179 - +1.0 5,404,389 + +2.7 5,404,521 - PP_4748 0.13 -0.5 5,404,521 - PP_4748 0.13 -0.2 5,404,564 + PP_4748 0.18 -0.1 5,404,747 + PP_4748 0.41 -1.1 5,404,760 + PP_4748 0.43 -1.9 5,404,850 - PP_4748 0.54 -0.7 5,404,885 + PP_4748 0.58 -0.6 5,404,885 + PP_4748 0.58 -1.6 5,404,885 + PP_4748 0.58 -0.4 5,404,886 - PP_4748 0.58 -1.4 5,404,933 + PP_4748 0.64 -0.6 5,405,113 + PP_4748 0.87 +0.6 5,405,113 + PP_4748 0.87 -0.8 5,405,114 - PP_4748 0.87 -0.1 5,405,114 - PP_4748 0.87 +0.0 5,405,185 + +1.1 5,405,269 + -1.1 5,405,269 + +0.3 5,405,269 + +0.1 5,405,270 - +1.3 5,405,270 - -1.2 5,405,311 + -0.3 5,405,311 + -1.3 5,405,312 - -0.7 5,405,383 + PP_4749 0.11 -0.5 5,405,383 + PP_4749 0.11 -0.3 5,405,384 - PP_4749 0.11 +2.7 5,405,384 - PP_4749 0.11 -0.3 5,405,384 - PP_4749 0.11 -0.6 5,405,384 - PP_4749 0.11 -0.4 5,405,384 - PP_4749 0.11 +0.1 5,405,416 + PP_4749 0.16 -0.0 5,405,417 - PP_4749 0.16 -0.2 5,405,417 - PP_4749 0.16 -1.0 5,405,417 - PP_4749 0.16 -0.4 5,405,417 - PP_4749 0.16 +0.2 5,405,417 - PP_4749 0.16 +0.0 5,405,417 - PP_4749 0.16 -2.1 5,405,665 + PP_4749 0.54 -0.2 5,405,666 - PP_4749 0.54 +1.2 5,405,752 + PP_4749 0.67 -0.5 5,405,752 + PP_4749 0.67 -1.2 5,405,753 - PP_4749 0.67 +0.4 5,405,753 - PP_4749 0.67 +0.4 5,405,753 - PP_4749 0.67 +0.3 5,406,042 + PP_4750 0.12 -0.6 5,406,042 + PP_4750 0.12 +0.3 5,406,174 + PP_4750 0.32 -0.9 5,406,174 + PP_4750 0.32 -0.7 5,406,175 - PP_4750 0.33 +1.1 5,406,175 - PP_4750 0.33 -0.7 5,406,175 - PP_4750 0.33 -0.6 5,406,200 - PP_4750 0.36 +0.1
Or see this region's nucleotide sequence