Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4694 and PP_5704 are separated by 242 nucleotides PP_5704 and PP_4695 overlap by 17 nucleotides PP_4695 and PP_4696 are separated by 4 nucleotides
PP_4694: PP_4694 - Glutamyl-Q tRNA(Asp) synthetase, at 5,332,266 to 5,333,168
_4694
PP_5704: PP_5704 - conserved protein of unknown function, at 5,333,411 to 5,333,587
_5704
PP_4695: PP_4695 - Sensory box histidine kinase, at 5,333,571 to 5,336,546
_4695
PP_4696: PP_4696 - Nitrogen regulation protein NR(I), at 5,336,551 to 5,337,993
_4696
Position (kb) 5333
5334
5335
5336
5337 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5332.611 kb on + strand, within PP_4694 at 5332.611 kb on + strand, within PP_4694 at 5332.612 kb on - strand, within PP_4694 at 5332.612 kb on - strand, within PP_4694 at 5332.623 kb on + strand, within PP_4694 at 5332.623 kb on + strand, within PP_4694 at 5332.623 kb on + strand, within PP_4694 at 5332.624 kb on - strand, within PP_4694 at 5332.624 kb on - strand, within PP_4694 at 5332.624 kb on - strand, within PP_4694 at 5332.698 kb on + strand, within PP_4694 at 5332.698 kb on + strand, within PP_4694 at 5332.728 kb on + strand, within PP_4694 at 5332.728 kb on + strand, within PP_4694 at 5332.788 kb on + strand, within PP_4694 at 5332.789 kb on - strand, within PP_4694 at 5332.789 kb on - strand, within PP_4694 at 5332.789 kb on - strand, within PP_4694 at 5332.789 kb on - strand, within PP_4694 at 5332.795 kb on - strand, within PP_4694 at 5332.795 kb on - strand, within PP_4694 at 5332.795 kb on - strand, within PP_4694 at 5332.887 kb on + strand, within PP_4694 at 5332.888 kb on - strand, within PP_4694 at 5332.888 kb on - strand, within PP_4694 at 5332.931 kb on + strand, within PP_4694 at 5332.931 kb on + strand, within PP_4694 at 5332.931 kb on + strand, within PP_4694 at 5332.931 kb on + strand, within PP_4694 at 5332.932 kb on - strand, within PP_4694 at 5332.932 kb on - strand, within PP_4694 at 5333.174 kb on + strand at 5333.344 kb on - strand at 5333.373 kb on + strand at 5333.373 kb on + strand at 5333.374 kb on - strand at 5333.374 kb on - strand at 5333.374 kb on - strand at 5333.414 kb on + strand at 5333.414 kb on + strand at 5333.414 kb on + strand at 5333.414 kb on + strand at 5333.414 kb on + strand at 5333.414 kb on + strand at 5333.414 kb on + strand at 5333.414 kb on + strand at 5333.414 kb on + strand at 5333.414 kb on + strand at 5333.414 kb on + strand at 5333.415 kb on - strand at 5333.415 kb on - strand at 5333.415 kb on - strand at 5333.415 kb on - strand at 5333.421 kb on - strand at 5333.453 kb on + strand, within PP_5704 at 5333.453 kb on + strand, within PP_5704 at 5333.454 kb on - strand, within PP_5704 at 5333.454 kb on - strand, within PP_5704 at 5333.454 kb on - strand, within PP_5704 at 5333.454 kb on - strand, within PP_5704 at 5333.516 kb on + strand, within PP_5704 at 5333.569 kb on - strand, within PP_5704 at 5333.585 kb on + strand at 5333.585 kb on + strand at 5333.620 kb on - strand at 5333.696 kb on + strand at 5333.696 kb on + strand at 5333.696 kb on + strand at 5333.696 kb on + strand at 5333.697 kb on - strand at 5333.697 kb on - strand at 5333.697 kb on - strand at 5333.700 kb on + strand at 5333.700 kb on + strand at 5333.700 kb on + strand at 5333.700 kb on + strand at 5333.701 kb on - strand at 5333.701 kb on - strand at 5333.701 kb on - strand at 5333.701 kb on - strand at 5333.701 kb on - strand at 5333.701 kb on - strand at 5333.701 kb on - strand at 5333.721 kb on + strand at 5333.722 kb on - strand at 5333.722 kb on - strand at 5333.742 kb on + strand at 5333.742 kb on + strand at 5333.742 kb on + strand at 5333.742 kb on + strand at 5333.742 kb on + strand at 5333.743 kb on - strand at 5333.743 kb on - strand at 5333.743 kb on - strand at 5333.743 kb on - strand at 5333.743 kb on - strand at 5333.763 kb on + strand at 5333.763 kb on + strand at 5333.763 kb on + strand at 5333.764 kb on - strand at 5333.764 kb on - strand at 5333.764 kb on - strand at 5333.769 kb on + strand at 5333.865 kb on + strand at 5333.865 kb on + strand at 5333.866 kb on - strand at 5333.866 kb on - strand at 5333.866 kb on - strand at 5333.913 kb on - strand, within PP_4695 at 5334.005 kb on + strand, within PP_4695 at 5334.186 kb on + strand, within PP_4695 at 5334.186 kb on + strand, within PP_4695 at 5334.186 kb on + strand, within PP_4695 at 5334.187 kb on - strand, within PP_4695 at 5334.187 kb on - strand, within PP_4695 at 5334.187 kb on - strand, within PP_4695 at 5334.195 kb on + strand, within PP_4695 at 5334.195 kb on + strand, within PP_4695 at 5334.196 kb on - strand, within PP_4695 at 5334.196 kb on - strand, within PP_4695 at 5334.196 kb on - strand, within PP_4695 at 5334.196 kb on - strand, within PP_4695 at 5334.196 kb on - strand, within PP_4695 at 5334.202 kb on + strand, within PP_4695 at 5334.345 kb on + strand, within PP_4695 at 5334.345 kb on + strand, within PP_4695 at 5334.345 kb on + strand, within PP_4695 at 5334.345 kb on + strand, within PP_4695 at 5334.345 kb on + strand, within PP_4695 at 5334.346 kb on - strand, within PP_4695 at 5334.346 kb on - strand, within PP_4695 at 5334.346 kb on - strand, within PP_4695 at 5334.346 kb on - strand, within PP_4695 at 5334.346 kb on - strand, within PP_4695 at 5334.346 kb on - strand, within PP_4695 at 5334.492 kb on + strand, within PP_4695 at 5334.550 kb on - strand, within PP_4695 at 5334.669 kb on + strand, within PP_4695 at 5334.669 kb on + strand, within PP_4695 at 5334.669 kb on + strand, within PP_4695 at 5334.669 kb on + strand, within PP_4695 at 5334.670 kb on - strand, within PP_4695 at 5334.670 kb on - strand, within PP_4695 at 5334.735 kb on + strand, within PP_4695 at 5334.736 kb on - strand, within PP_4695 at 5334.750 kb on + strand, within PP_4695 at 5334.751 kb on - strand, within PP_4695 at 5334.826 kb on + strand, within PP_4695 at 5334.827 kb on - strand, within PP_4695 at 5334.831 kb on + strand, within PP_4695 at 5334.832 kb on - strand, within PP_4695 at 5334.939 kb on + strand, within PP_4695 at 5334.939 kb on + strand, within PP_4695 at 5334.939 kb on + strand, within PP_4695 at 5334.940 kb on - strand, within PP_4695 at 5334.963 kb on + strand, within PP_4695 at 5334.963 kb on + strand, within PP_4695 at 5334.963 kb on + strand, within PP_4695 at 5334.963 kb on + strand, within PP_4695 at 5334.963 kb on + strand, within PP_4695 at 5334.963 kb on + strand, within PP_4695 at 5334.963 kb on + strand, within PP_4695 at 5334.963 kb on + strand, within PP_4695 at 5334.964 kb on - strand, within PP_4695 at 5334.964 kb on - strand, within PP_4695 at 5334.964 kb on - strand, within PP_4695 at 5334.964 kb on - strand, within PP_4695 at 5334.964 kb on - strand, within PP_4695 at 5335.227 kb on + strand, within PP_4695 at 5335.350 kb on + strand, within PP_4695 at 5335.351 kb on - strand, within PP_4695 at 5335.371 kb on + strand, within PP_4695 at 5335.372 kb on - strand, within PP_4695 at 5335.372 kb on - strand, within PP_4695 at 5336.125 kb on + strand, within PP_4695 at 5336.125 kb on + strand, within PP_4695 at 5336.125 kb on + strand, within PP_4695 at 5336.400 kb on + strand at 5336.400 kb on + strand at 5336.401 kb on - strand at 5336.401 kb on - strand at 5336.421 kb on + strand at 5336.422 kb on - strand at 5336.422 kb on - strand at 5336.782 kb on + strand, within PP_4696 at 5336.782 kb on + strand, within PP_4696 at 5336.833 kb on + strand, within PP_4696 at 5336.834 kb on - strand, within PP_4696 at 5336.884 kb on - strand, within PP_4696 at 5336.955 kb on + strand, within PP_4696 at 5336.955 kb on + strand, within PP_4696 at 5336.956 kb on - strand, within PP_4696 at 5336.956 kb on - strand, within PP_4696 at 5336.956 kb on - strand, within PP_4696 at 5337.037 kb on + strand, within PP_4696 at 5337.037 kb on + strand, within PP_4696 at 5337.037 kb on + strand, within PP_4696 at 5337.038 kb on - strand, within PP_4696 at 5337.038 kb on - strand, within PP_4696 at 5337.038 kb on - strand, within PP_4696 at 5337.038 kb on - strand, within PP_4696 at 5337.368 kb on + strand, within PP_4696
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 5,332,611 + PP_4694 0.38 +0.6 5,332,611 + PP_4694 0.38 -0.3 5,332,612 - PP_4694 0.38 +0.8 5,332,612 - PP_4694 0.38 -1.0 5,332,623 + PP_4694 0.40 +2.7 5,332,623 + PP_4694 0.40 +0.2 5,332,623 + PP_4694 0.40 -0.2 5,332,624 - PP_4694 0.40 -0.4 5,332,624 - PP_4694 0.40 -0.0 5,332,624 - PP_4694 0.40 +0.7 5,332,698 + PP_4694 0.48 +0.3 5,332,698 + PP_4694 0.48 +0.3 5,332,728 + PP_4694 0.51 +0.6 5,332,728 + PP_4694 0.51 -1.7 5,332,788 + PP_4694 0.58 -0.3 5,332,789 - PP_4694 0.58 +0.6 5,332,789 - PP_4694 0.58 -1.2 5,332,789 - PP_4694 0.58 +0.1 5,332,789 - PP_4694 0.58 -0.3 5,332,795 - PP_4694 0.59 -0.7 5,332,795 - PP_4694 0.59 +0.1 5,332,795 - PP_4694 0.59 -0.5 5,332,887 + PP_4694 0.69 +1.0 5,332,888 - PP_4694 0.69 +0.2 5,332,888 - PP_4694 0.69 +0.3 5,332,931 + PP_4694 0.74 +1.5 5,332,931 + PP_4694 0.74 -0.5 5,332,931 + PP_4694 0.74 +1.7 5,332,931 + PP_4694 0.74 -3.5 5,332,932 - PP_4694 0.74 +2.7 5,332,932 - PP_4694 0.74 -1.1 5,333,174 + +0.3 5,333,344 - -2.9 5,333,373 + +0.4 5,333,373 + -0.9 5,333,374 - +0.7 5,333,374 - -1.1 5,333,374 - -1.7 5,333,414 + -1.4 5,333,414 + -0.2 5,333,414 + +0.3 5,333,414 + +0.2 5,333,414 + +0.5 5,333,414 + -0.1 5,333,414 + +0.7 5,333,414 + +0.1 5,333,414 + -0.6 5,333,414 + +0.1 5,333,414 + +0.3 5,333,415 - -1.2 5,333,415 - +0.0 5,333,415 - +0.7 5,333,415 - -1.0 5,333,421 - +0.1 5,333,453 + PP_5704 0.24 -0.7 5,333,453 + PP_5704 0.24 +0.0 5,333,454 - PP_5704 0.24 +0.1 5,333,454 - PP_5704 0.24 +0.1 5,333,454 - PP_5704 0.24 -0.2 5,333,454 - PP_5704 0.24 +0.7 5,333,516 + PP_5704 0.59 -0.5 5,333,569 - PP_5704 0.89 -0.8 5,333,585 + -0.9 5,333,585 + -0.3 5,333,620 - -1.4 5,333,696 + +0.7 5,333,696 + +1.7 5,333,696 + -0.9 5,333,696 + +1.1 5,333,697 - +0.3 5,333,697 - +1.1 5,333,697 - -2.1 5,333,700 + -0.5 5,333,700 + -1.2 5,333,700 + -2.5 5,333,700 + +1.1 5,333,701 - -1.3 5,333,701 - -0.8 5,333,701 - -0.3 5,333,701 - -0.7 5,333,701 - -1.8 5,333,701 - -0.9 5,333,701 - -0.8 5,333,721 + -0.9 5,333,722 - +0.1 5,333,722 - -0.8 5,333,742 + +0.2 5,333,742 + -1.3 5,333,742 + +1.1 5,333,742 + -0.5 5,333,742 + -0.5 5,333,743 - -1.2 5,333,743 - -2.1 5,333,743 - +0.7 5,333,743 - -0.9 5,333,743 - +2.7 5,333,763 + -2.8 5,333,763 + +0.3 5,333,763 + +0.1 5,333,764 - +0.3 5,333,764 - +0.1 5,333,764 - -1.6 5,333,769 + +1.7 5,333,865 + +0.1 5,333,865 + -1.1 5,333,866 - -0.8 5,333,866 - -0.8 5,333,866 - +1.7 5,333,913 - PP_4695 0.11 +1.7 5,334,005 + PP_4695 0.15 -0.7 5,334,186 + PP_4695 0.21 +0.8 5,334,186 + PP_4695 0.21 +0.1 5,334,186 + PP_4695 0.21 -0.7 5,334,187 - PP_4695 0.21 +1.7 5,334,187 - PP_4695 0.21 -0.3 5,334,187 - PP_4695 0.21 +0.7 5,334,195 + PP_4695 0.21 +0.6 5,334,195 + PP_4695 0.21 -1.1 5,334,196 - PP_4695 0.21 +1.1 5,334,196 - PP_4695 0.21 -2.7 5,334,196 - PP_4695 0.21 -2.2 5,334,196 - PP_4695 0.21 -1.8 5,334,196 - PP_4695 0.21 -0.8 5,334,202 + PP_4695 0.21 -0.1 5,334,345 + PP_4695 0.26 -0.1 5,334,345 + PP_4695 0.26 -2.3 5,334,345 + PP_4695 0.26 -0.4 5,334,345 + PP_4695 0.26 -0.7 5,334,345 + PP_4695 0.26 -0.3 5,334,346 - PP_4695 0.26 -0.3 5,334,346 - PP_4695 0.26 -0.1 5,334,346 - PP_4695 0.26 +0.7 5,334,346 - PP_4695 0.26 +1.1 5,334,346 - PP_4695 0.26 +1.1 5,334,346 - PP_4695 0.26 +1.1 5,334,492 + PP_4695 0.31 -0.7 5,334,550 - PP_4695 0.33 +0.7 5,334,669 + PP_4695 0.37 -0.9 5,334,669 + PP_4695 0.37 -0.4 5,334,669 + PP_4695 0.37 -0.6 5,334,669 + PP_4695 0.37 -0.9 5,334,670 - PP_4695 0.37 +1.1 5,334,670 - PP_4695 0.37 +0.2 5,334,735 + PP_4695 0.39 -1.7 5,334,736 - PP_4695 0.39 -1.4 5,334,750 + PP_4695 0.40 -0.3 5,334,751 - PP_4695 0.40 +0.1 5,334,826 + PP_4695 0.42 -0.1 5,334,827 - PP_4695 0.42 +1.1 5,334,831 + PP_4695 0.42 +1.7 5,334,832 - PP_4695 0.42 -0.5 5,334,939 + PP_4695 0.46 +1.1 5,334,939 + PP_4695 0.46 +0.8 5,334,939 + PP_4695 0.46 -2.2 5,334,940 - PP_4695 0.46 -0.3 5,334,963 + PP_4695 0.47 -1.1 5,334,963 + PP_4695 0.47 -0.7 5,334,963 + PP_4695 0.47 -2.5 5,334,963 + PP_4695 0.47 +1.1 5,334,963 + PP_4695 0.47 -0.3 5,334,963 + PP_4695 0.47 -2.6 5,334,963 + PP_4695 0.47 +0.3 5,334,963 + PP_4695 0.47 +0.3 5,334,964 - PP_4695 0.47 -1.4 5,334,964 - PP_4695 0.47 -1.0 5,334,964 - PP_4695 0.47 +0.7 5,334,964 - PP_4695 0.47 +0.3 5,334,964 - PP_4695 0.47 +1.1 5,335,227 + PP_4695 0.56 -1.5 5,335,350 + PP_4695 0.60 -0.3 5,335,351 - PP_4695 0.60 +0.3 5,335,371 + PP_4695 0.60 -1.0 5,335,372 - PP_4695 0.61 -0.1 5,335,372 - PP_4695 0.61 -0.3 5,336,125 + PP_4695 0.86 +1.1 5,336,125 + PP_4695 0.86 -1.7 5,336,125 + PP_4695 0.86 -0.5 5,336,400 + +2.7 5,336,400 + -1.3 5,336,401 - -0.5 5,336,401 - -0.3 5,336,421 + -0.5 5,336,422 - -0.7 5,336,422 - +0.3 5,336,782 + PP_4696 0.16 +0.7 5,336,782 + PP_4696 0.16 +0.1 5,336,833 + PP_4696 0.20 +0.3 5,336,834 - PP_4696 0.20 -0.3 5,336,884 - PP_4696 0.23 +2.7 5,336,955 + PP_4696 0.28 +1.7 5,336,955 + PP_4696 0.28 +1.7 5,336,956 - PP_4696 0.28 +0.3 5,336,956 - PP_4696 0.28 +0.7 5,336,956 - PP_4696 0.28 +0.7 5,337,037 + PP_4696 0.34 +0.3 5,337,037 + PP_4696 0.34 +0.1 5,337,037 + PP_4696 0.34 +2.7 5,337,038 - PP_4696 0.34 +2.7 5,337,038 - PP_4696 0.34 +1.7 5,337,038 - PP_4696 0.34 +1.3 5,337,038 - PP_4696 0.34 -0.5 5,337,368 + PP_4696 0.57 +2.7
Or see this region's nucleotide sequence