Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4423 and PP_4424 are separated by 202 nucleotides PP_4424 and PP_4425 are separated by 50 nucleotides PP_4425 and PP_4426 are separated by 98 nucleotides
PP_4423: PP_4423 - conserved protein of unknown function, at 5,019,116 to 5,020,111
_4423
PP_4424: PP_4424 - Uncharacterized HTH-type transcriptional regulator y4tD, at 5,020,314 to 5,020,781
_4424
PP_4425: PP_4425 - putative amino-acid ABC transporter ATP-binding protein y4tH, at 5,020,832 to 5,021,599
_4425
PP_4426: PP_4426 - Amino acid ABC transporter, permease protein, at 5,021,698 to 5,022,354
_4426
Position (kb) 5020
5021 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5019.383 kb on - strand, within PP_4423 at 5019.491 kb on - strand, within PP_4423 at 5019.527 kb on - strand, within PP_4423 at 5019.570 kb on + strand, within PP_4423 at 5019.634 kb on + strand, within PP_4423 at 5019.729 kb on + strand, within PP_4423 at 5019.779 kb on - strand, within PP_4423 at 5019.927 kb on + strand, within PP_4423 at 5020.116 kb on + strand at 5020.117 kb on - strand at 5020.123 kb on + strand at 5020.142 kb on + strand at 5020.215 kb on + strand at 5020.246 kb on + strand at 5020.255 kb on + strand at 5020.295 kb on + strand at 5020.433 kb on + strand, within PP_4424 at 5020.608 kb on + strand, within PP_4424 at 5020.655 kb on + strand, within PP_4424 at 5020.780 kb on - strand at 5020.935 kb on + strand, within PP_4425 at 5020.935 kb on + strand, within PP_4425 at 5020.936 kb on - strand, within PP_4425 at 5020.988 kb on - strand, within PP_4425 at 5020.993 kb on + strand, within PP_4425 at 5020.993 kb on + strand, within PP_4425 at 5020.993 kb on + strand, within PP_4425 at 5020.994 kb on - strand, within PP_4425 at 5020.994 kb on - strand, within PP_4425 at 5020.994 kb on - strand, within PP_4425 at 5021.008 kb on + strand, within PP_4425 at 5021.009 kb on - strand, within PP_4425 at 5021.009 kb on - strand, within PP_4425 at 5021.126 kb on + strand, within PP_4425 at 5021.127 kb on - strand, within PP_4425 at 5021.169 kb on + strand, within PP_4425 at 5021.243 kb on + strand at 5021.291 kb on - strand, within PP_4425 at 5021.321 kb on + strand, within PP_4425 at 5021.321 kb on + strand, within PP_4425 at 5021.322 kb on - strand, within PP_4425 at 5021.322 kb on - strand, within PP_4425 at 5021.322 kb on - strand, within PP_4425 at 5021.330 kb on + strand, within PP_4425 at 5021.330 kb on + strand, within PP_4425 at 5021.330 kb on + strand, within PP_4425 at 5021.569 kb on - strand at 5021.593 kb on + strand at 5021.593 kb on + strand at 5021.667 kb on - strand at 5021.700 kb on - strand at 5021.777 kb on - strand, within PP_4426
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 5,019,383 - PP_4423 0.27 +2.4 5,019,491 - PP_4423 0.38 -0.1 5,019,527 - PP_4423 0.41 -0.4 5,019,570 + PP_4423 0.46 -1.2 5,019,634 + PP_4423 0.52 +0.7 5,019,729 + PP_4423 0.62 -0.2 5,019,779 - PP_4423 0.67 +1.0 5,019,927 + PP_4423 0.81 +0.6 5,020,116 + -0.7 5,020,117 - +0.8 5,020,123 + -0.3 5,020,142 + -1.5 5,020,215 + -3.4 5,020,246 + -1.4 5,020,255 + -0.4 5,020,295 + +2.2 5,020,433 + PP_4424 0.25 -0.9 5,020,608 + PP_4424 0.63 -1.5 5,020,655 + PP_4424 0.73 -1.3 5,020,780 - -0.6 5,020,935 + PP_4425 0.13 -0.5 5,020,935 + PP_4425 0.13 +0.0 5,020,936 - PP_4425 0.14 -1.1 5,020,988 - PP_4425 0.20 -1.1 5,020,993 + PP_4425 0.21 +0.7 5,020,993 + PP_4425 0.21 +0.3 5,020,993 + PP_4425 0.21 +0.7 5,020,994 - PP_4425 0.21 -0.3 5,020,994 - PP_4425 0.21 +0.8 5,020,994 - PP_4425 0.21 -1.3 5,021,008 + PP_4425 0.23 -1.3 5,021,009 - PP_4425 0.23 +0.4 5,021,009 - PP_4425 0.23 +1.7 5,021,126 + PP_4425 0.38 -0.4 5,021,127 - PP_4425 0.38 -0.5 5,021,169 + PP_4425 0.44 +1.0 5,021,243 + -0.1 5,021,291 - PP_4425 0.60 -0.0 5,021,321 + PP_4425 0.64 -0.1 5,021,321 + PP_4425 0.64 +1.7 5,021,322 - PP_4425 0.64 -0.9 5,021,322 - PP_4425 0.64 -0.5 5,021,322 - PP_4425 0.64 -0.8 5,021,330 + PP_4425 0.65 -0.1 5,021,330 + PP_4425 0.65 +0.8 5,021,330 + PP_4425 0.65 -0.2 5,021,569 - -0.2 5,021,593 + -0.8 5,021,593 + +0.2 5,021,667 - +0.1 5,021,700 - +0.5 5,021,777 - PP_4426 0.12 -0.2
Or see this region's nucleotide sequence