Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3889 and PP_3890 overlap by 4 nucleotides PP_3890 and PP_3891 are separated by 148 nucleotides PP_3891 and PP_3892 overlap by 19 nucleotides PP_3892 and PP_3893 overlap by 1 nucleotides
PP_3889: PP_3889 - putative Phage integrase, at 4,406,545 to 4,407,843
_3889
PP_3890: PP_3890 - conserved protein of unknown function, at 4,407,840 to 4,408,283
_3890
PP_3891: PP_3891 - conserved protein of unknown function, at 4,408,432 to 4,408,728
_3891
PP_3892: PP_3892 - conserved protein of unknown function, at 4,408,710 to 4,408,916
_3892
PP_3893: PP_3893 - putative Phage DNA helicase, at 4,408,916 to 4,410,313
_3893
Position (kb) 4408
4409 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4407.740 kb on + strand at 4407.741 kb on - strand at 4407.871 kb on + strand at 4407.872 kb on - strand at 4407.872 kb on - strand at 4407.925 kb on + strand, within PP_3890 at 4407.926 kb on - strand, within PP_3890 at 4407.953 kb on - strand, within PP_3890 at 4407.953 kb on - strand, within PP_3890 at 4408.012 kb on + strand, within PP_3890 at 4408.012 kb on + strand, within PP_3890 at 4408.013 kb on - strand, within PP_3890 at 4408.013 kb on - strand, within PP_3890 at 4408.013 kb on - strand, within PP_3890 at 4408.013 kb on - strand, within PP_3890 at 4408.024 kb on + strand, within PP_3890 at 4408.024 kb on + strand, within PP_3890 at 4408.025 kb on - strand, within PP_3890 at 4408.135 kb on + strand, within PP_3890 at 4408.135 kb on + strand, within PP_3890 at 4408.136 kb on - strand, within PP_3890 at 4408.136 kb on - strand, within PP_3890 at 4408.147 kb on + strand, within PP_3890 at 4408.148 kb on - strand, within PP_3890 at 4408.148 kb on - strand, within PP_3890 at 4408.268 kb on + strand at 4408.269 kb on - strand at 4408.269 kb on - strand at 4408.303 kb on + strand at 4408.303 kb on + strand at 4408.303 kb on + strand at 4408.304 kb on - strand at 4408.423 kb on + strand at 4408.424 kb on - strand at 4408.429 kb on + strand at 4408.429 kb on + strand at 4408.430 kb on - strand at 4408.430 kb on - strand at 4408.519 kb on + strand, within PP_3891 at 4408.625 kb on + strand, within PP_3891 at 4408.625 kb on + strand, within PP_3891 at 4408.625 kb on + strand, within PP_3891 at 4408.888 kb on + strand, within PP_3892 at 4409.306 kb on - strand, within PP_3893 at 4409.306 kb on - strand, within PP_3893 at 4409.370 kb on + strand, within PP_3893 at 4409.370 kb on + strand, within PP_3893 at 4409.370 kb on + strand, within PP_3893 at 4409.371 kb on - strand, within PP_3893 at 4409.371 kb on - strand, within PP_3893 at 4409.371 kb on - strand, within PP_3893 at 4409.371 kb on - strand, within PP_3893 at 4409.371 kb on - strand, within PP_3893 at 4409.468 kb on + strand, within PP_3893 at 4409.468 kb on + strand, within PP_3893 at 4409.468 kb on + strand, within PP_3893 at 4409.468 kb on + strand, within PP_3893 at 4409.469 kb on - strand, within PP_3893 at 4409.469 kb on - strand, within PP_3893 at 4409.564 kb on - strand, within PP_3893 at 4409.662 kb on - strand, within PP_3893 at 4409.791 kb on + strand, within PP_3893
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 4,407,740 + +0.7 4,407,741 - -0.7 4,407,871 + +1.2 4,407,872 - -0.1 4,407,872 - -1.1 4,407,925 + PP_3890 0.19 +0.6 4,407,926 - PP_3890 0.19 -1.2 4,407,953 - PP_3890 0.25 +0.5 4,407,953 - PP_3890 0.25 -0.0 4,408,012 + PP_3890 0.39 -0.5 4,408,012 + PP_3890 0.39 +2.3 4,408,013 - PP_3890 0.39 +0.9 4,408,013 - PP_3890 0.39 +0.0 4,408,013 - PP_3890 0.39 +1.5 4,408,013 - PP_3890 0.39 +0.9 4,408,024 + PP_3890 0.41 -0.7 4,408,024 + PP_3890 0.41 +0.2 4,408,025 - PP_3890 0.42 -1.1 4,408,135 + PP_3890 0.66 +0.3 4,408,135 + PP_3890 0.66 +0.2 4,408,136 - PP_3890 0.67 -0.4 4,408,136 - PP_3890 0.67 -0.2 4,408,147 + PP_3890 0.69 -1.2 4,408,148 - PP_3890 0.69 -0.1 4,408,148 - PP_3890 0.69 +1.4 4,408,268 + -1.7 4,408,269 - +1.2 4,408,269 - +1.8 4,408,303 + +1.4 4,408,303 + +0.4 4,408,303 + -1.0 4,408,304 - -0.7 4,408,423 + -2.2 4,408,424 - +0.5 4,408,429 + +0.4 4,408,429 + -0.1 4,408,430 - -0.4 4,408,430 - -0.4 4,408,519 + PP_3891 0.29 +0.4 4,408,625 + PP_3891 0.65 -0.1 4,408,625 + PP_3891 0.65 -0.7 4,408,625 + PP_3891 0.65 +0.2 4,408,888 + PP_3892 0.86 -2.5 4,409,306 - PP_3893 0.28 -0.5 4,409,306 - PP_3893 0.28 +1.1 4,409,370 + PP_3893 0.32 +0.9 4,409,370 + PP_3893 0.32 +0.3 4,409,370 + PP_3893 0.32 +0.1 4,409,371 - PP_3893 0.33 +0.5 4,409,371 - PP_3893 0.33 -0.8 4,409,371 - PP_3893 0.33 +0.4 4,409,371 - PP_3893 0.33 +1.5 4,409,371 - PP_3893 0.33 +0.2 4,409,468 + PP_3893 0.39 +1.4 4,409,468 + PP_3893 0.39 -0.4 4,409,468 + PP_3893 0.39 -0.4 4,409,468 + PP_3893 0.39 -1.5 4,409,469 - PP_3893 0.40 +0.2 4,409,469 - PP_3893 0.40 -0.6 4,409,564 - PP_3893 0.46 +1.2 4,409,662 - PP_3893 0.53 +1.4 4,409,791 + PP_3893 0.63 -0.7
Or see this region's nucleotide sequence