Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3850 and PP_5637 overlap by 22 nucleotides PP_5637 and PP_3851 are separated by 109 nucleotides PP_3851 and PP_3852 are separated by 115 nucleotides PP_3852 and PP_5638 are separated by 74 nucleotides PP_5638 and PP_3854 overlap by 4 nucleotides
PP_3850: PP_3850 - conserved protein of unknown function, at 4,375,036 to 4,375,299
_3850
PP_5637: PP_5637 - conserved protein of unknown function, at 4,375,278 to 4,375,535
_5637
PP_3851: PP_3851 - conserved protein of unknown function, at 4,375,645 to 4,375,968
_3851
PP_3852: PP_3852 - conserved protein of unknown function with BNR domain, at 4,376,084 to 4,377,571
_3852
PP_5638: PP_5638 - conserved protein of unknown function, at 4,377,646 to 4,378,176
_5638
PP_3854: PP_3854 - Lysozyme, at 4,378,173 to 4,378,712
_3854
Position (kb) 4376
4377
4378 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 4375.178 kb on + strand, within PP_3850 at 4375.179 kb on - strand, within PP_3850 at 4375.222 kb on + strand, within PP_3850 at 4375.223 kb on - strand, within PP_3850 at 4375.225 kb on + strand, within PP_3850 at 4375.225 kb on + strand, within PP_3850 at 4375.225 kb on + strand, within PP_3850 at 4375.226 kb on - strand, within PP_3850 at 4375.309 kb on + strand, within PP_5637 at 4375.309 kb on + strand, within PP_5637 at 4375.309 kb on + strand, within PP_5637 at 4375.311 kb on + strand, within PP_5637 at 4375.311 kb on + strand, within PP_5637 at 4375.311 kb on + strand, within PP_5637 at 4375.311 kb on + strand, within PP_5637 at 4375.311 kb on + strand, within PP_5637 at 4375.311 kb on + strand, within PP_5637 at 4375.311 kb on + strand, within PP_5637 at 4375.311 kb on + strand, within PP_5637 at 4375.311 kb on + strand, within PP_5637 at 4375.312 kb on - strand, within PP_5637 at 4375.312 kb on - strand, within PP_5637 at 4375.312 kb on - strand, within PP_5637 at 4375.312 kb on - strand, within PP_5637 at 4375.312 kb on - strand, within PP_5637 at 4375.312 kb on - strand, within PP_5637 at 4375.312 kb on - strand, within PP_5637 at 4375.312 kb on - strand, within PP_5637 at 4375.312 kb on - strand, within PP_5637 at 4375.332 kb on + strand, within PP_5637 at 4375.332 kb on + strand, within PP_5637 at 4375.397 kb on + strand, within PP_5637 at 4375.398 kb on - strand, within PP_5637 at 4375.421 kb on + strand, within PP_5637 at 4375.421 kb on + strand, within PP_5637 at 4375.567 kb on + strand at 4375.567 kb on + strand at 4375.567 kb on + strand at 4375.567 kb on + strand at 4375.567 kb on + strand at 4375.568 kb on - strand at 4375.568 kb on - strand at 4375.568 kb on - strand at 4375.568 kb on - strand at 4375.568 kb on - strand at 4375.606 kb on - strand at 4375.607 kb on + strand at 4375.608 kb on - strand at 4375.790 kb on + strand, within PP_3851 at 4375.901 kb on + strand, within PP_3851 at 4375.901 kb on + strand, within PP_3851 at 4375.902 kb on - strand, within PP_3851 at 4375.902 kb on - strand, within PP_3851 at 4375.903 kb on + strand, within PP_3851 at 4375.903 kb on + strand at 4375.903 kb on + strand, within PP_3851 at 4375.903 kb on + strand, within PP_3851 at 4375.904 kb on - strand, within PP_3851 at 4375.904 kb on - strand, within PP_3851 at 4375.904 kb on - strand, within PP_3851 at 4375.904 kb on - strand, within PP_3851 at 4375.921 kb on + strand, within PP_3851 at 4375.922 kb on - strand, within PP_3851 at 4375.947 kb on + strand at 4375.948 kb on - strand at 4375.964 kb on - strand at 4375.979 kb on + strand at 4375.979 kb on + strand at 4375.980 kb on - strand at 4375.980 kb on - strand at 4375.993 kb on + strand at 4375.993 kb on + strand at 4376.005 kb on + strand at 4376.005 kb on + strand at 4376.005 kb on + strand at 4376.006 kb on - strand at 4376.006 kb on - strand at 4376.006 kb on - strand at 4376.006 kb on - strand at 4376.009 kb on + strand at 4376.009 kb on + strand at 4376.009 kb on + strand at 4376.009 kb on + strand at 4376.055 kb on + strand at 4376.056 kb on - strand at 4376.066 kb on + strand at 4376.066 kb on + strand at 4376.066 kb on + strand at 4376.067 kb on - strand at 4376.067 kb on - strand at 4376.067 kb on - strand at 4376.067 kb on - strand at 4376.067 kb on - strand at 4376.067 kb on - strand at 4376.079 kb on - strand at 4376.079 kb on - strand at 4376.214 kb on - strand at 4376.214 kb on - strand at 4376.260 kb on + strand, within PP_3852 at 4376.296 kb on - strand, within PP_3852 at 4376.296 kb on - strand, within PP_3852 at 4376.367 kb on - strand, within PP_3852 at 4376.393 kb on + strand, within PP_3852 at 4376.393 kb on + strand, within PP_3852 at 4376.433 kb on + strand, within PP_3852 at 4376.462 kb on + strand, within PP_3852 at 4376.463 kb on - strand, within PP_3852 at 4376.463 kb on - strand, within PP_3852 at 4376.481 kb on + strand, within PP_3852 at 4376.482 kb on - strand, within PP_3852 at 4376.514 kb on - strand, within PP_3852 at 4376.591 kb on - strand, within PP_3852 at 4376.644 kb on - strand, within PP_3852 at 4376.658 kb on - strand, within PP_3852 at 4376.708 kb on + strand, within PP_3852 at 4376.714 kb on + strand, within PP_3852 at 4376.714 kb on + strand at 4376.716 kb on + strand, within PP_3852 at 4376.718 kb on + strand, within PP_3852 at 4376.719 kb on - strand, within PP_3852 at 4376.719 kb on - strand, within PP_3852 at 4376.725 kb on + strand, within PP_3852 at 4376.821 kb on + strand, within PP_3852 at 4376.821 kb on + strand, within PP_3852 at 4376.822 kb on - strand, within PP_3852 at 4376.851 kb on - strand, within PP_3852 at 4376.879 kb on + strand, within PP_3852 at 4376.882 kb on + strand, within PP_3852 at 4376.882 kb on + strand, within PP_3852 at 4376.955 kb on + strand, within PP_3852 at 4376.960 kb on + strand, within PP_3852 at 4376.960 kb on + strand, within PP_3852 at 4376.960 kb on + strand, within PP_3852 at 4376.960 kb on + strand, within PP_3852 at 4376.960 kb on + strand, within PP_3852 at 4376.960 kb on + strand, within PP_3852 at 4376.961 kb on - strand, within PP_3852 at 4376.971 kb on - strand, within PP_3852 at 4376.978 kb on + strand, within PP_3852 at 4376.978 kb on + strand, within PP_3852 at 4377.004 kb on - strand, within PP_3852 at 4377.047 kb on - strand, within PP_3852 at 4377.115 kb on - strand, within PP_3852 at 4377.115 kb on - strand, within PP_3852 at 4377.129 kb on - strand, within PP_3852 at 4377.135 kb on + strand, within PP_3852 at 4377.135 kb on + strand, within PP_3852 at 4377.220 kb on + strand, within PP_3852 at 4377.232 kb on + strand, within PP_3852 at 4377.232 kb on + strand, within PP_3852 at 4377.253 kb on + strand, within PP_3852 at 4377.291 kb on + strand, within PP_3852 at 4377.291 kb on + strand, within PP_3852 at 4377.291 kb on + strand, within PP_3852 at 4377.291 kb on + strand, within PP_3852 at 4377.292 kb on - strand, within PP_3852 at 4377.292 kb on - strand, within PP_3852 at 4377.297 kb on - strand, within PP_3852 at 4377.297 kb on - strand, within PP_3852 at 4377.297 kb on - strand, within PP_3852 at 4377.339 kb on + strand, within PP_3852 at 4377.339 kb on + strand, within PP_3852 at 4377.339 kb on + strand, within PP_3852 at 4377.339 kb on + strand, within PP_3852 at 4377.345 kb on + strand, within PP_3852 at 4377.346 kb on - strand, within PP_3852 at 4377.376 kb on + strand, within PP_3852 at 4377.430 kb on - strand at 4377.502 kb on + strand at 4377.503 kb on - strand at 4377.503 kb on - strand at 4377.566 kb on + strand at 4377.566 kb on + strand at 4377.566 kb on + strand at 4377.566 kb on + strand at 4377.566 kb on + strand at 4377.567 kb on - strand at 4377.567 kb on - strand at 4377.585 kb on - strand at 4377.626 kb on + strand at 4377.626 kb on + strand at 4377.627 kb on - strand at 4377.627 kb on - strand at 4377.627 kb on - strand at 4377.641 kb on + strand at 4377.641 kb on + strand at 4377.641 kb on + strand at 4377.641 kb on + strand at 4377.642 kb on - strand at 4377.642 kb on - strand at 4377.642 kb on - strand at 4377.642 kb on - strand at 4377.642 kb on - strand at 4377.647 kb on + strand at 4377.647 kb on + strand at 4377.647 kb on + strand at 4377.647 kb on + strand at 4377.693 kb on + strand at 4377.693 kb on + strand at 4377.693 kb on + strand at 4377.693 kb on + strand at 4377.693 kb on + strand at 4377.694 kb on - strand at 4377.694 kb on - strand at 4377.694 kb on - strand at 4377.694 kb on - strand at 4377.695 kb on + strand at 4377.695 kb on + strand at 4377.695 kb on + strand at 4377.695 kb on + strand at 4377.695 kb on + strand at 4377.696 kb on - strand at 4377.696 kb on - strand at 4377.701 kb on + strand, within PP_5638 at 4377.701 kb on + strand, within PP_5638 at 4377.701 kb on + strand, within PP_5638 at 4377.701 kb on + strand, within PP_5638 at 4377.701 kb on + strand, within PP_5638 at 4377.702 kb on - strand, within PP_5638 at 4378.033 kb on + strand, within PP_5638 at 4378.034 kb on - strand, within PP_5638 at 4378.115 kb on + strand, within PP_5638 at 4378.258 kb on + strand, within PP_3854 at 4378.258 kb on + strand, within PP_3854 at 4378.258 kb on + strand, within PP_3854 at 4378.259 kb on - strand, within PP_3854 at 4378.456 kb on + strand, within PP_3854 at 4378.456 kb on + strand, within PP_3854 at 4378.456 kb on + strand, within PP_3854 at 4378.456 kb on + strand, within PP_3854 at 4378.457 kb on - strand, within PP_3854 at 4378.457 kb on - strand, within PP_3854 at 4378.519 kb on + strand, within PP_3854 at 4378.519 kb on - strand, within PP_3854 at 4378.520 kb on - strand, within PP_3854 at 4378.528 kb on + strand, within PP_3854 at 4378.567 kb on + strand, within PP_3854 at 4378.568 kb on - strand, within PP_3854
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 4,375,178 + PP_3850 0.54 +1.7 4,375,179 - PP_3850 0.54 -0.7 4,375,222 + PP_3850 0.70 +0.6 4,375,223 - PP_3850 0.71 +1.0 4,375,225 + PP_3850 0.72 +0.2 4,375,225 + PP_3850 0.72 +0.6 4,375,225 + PP_3850 0.72 -1.3 4,375,226 - PP_3850 0.72 -0.3 4,375,309 + PP_5637 0.12 -2.7 4,375,309 + PP_5637 0.12 +1.7 4,375,309 + PP_5637 0.12 -0.8 4,375,311 + PP_5637 0.13 +0.4 4,375,311 + PP_5637 0.13 -0.5 4,375,311 + PP_5637 0.13 -0.0 4,375,311 + PP_5637 0.13 -0.3 4,375,311 + PP_5637 0.13 +1.0 4,375,311 + PP_5637 0.13 +1.1 4,375,311 + PP_5637 0.13 +0.9 4,375,311 + PP_5637 0.13 +0.3 4,375,311 + PP_5637 0.13 +0.4 4,375,312 - PP_5637 0.13 +0.4 4,375,312 - PP_5637 0.13 +0.1 4,375,312 - PP_5637 0.13 -0.4 4,375,312 - PP_5637 0.13 -1.4 4,375,312 - PP_5637 0.13 +0.3 4,375,312 - PP_5637 0.13 +0.4 4,375,312 - PP_5637 0.13 -0.8 4,375,312 - PP_5637 0.13 -1.4 4,375,312 - PP_5637 0.13 +0.4 4,375,332 + PP_5637 0.21 +1.1 4,375,332 + PP_5637 0.21 +1.2 4,375,397 + PP_5637 0.46 +0.5 4,375,398 - PP_5637 0.47 +0.4 4,375,421 + PP_5637 0.55 -0.2 4,375,421 + PP_5637 0.55 +1.2 4,375,567 + +0.0 4,375,567 + -0.3 4,375,567 + +0.0 4,375,567 + +0.9 4,375,567 + -0.2 4,375,568 - +0.4 4,375,568 - +0.2 4,375,568 - +0.9 4,375,568 - +1.4 4,375,568 - -0.0 4,375,606 - +0.2 4,375,607 + +0.4 4,375,608 - -0.8 4,375,790 + PP_3851 0.45 -1.2 4,375,901 + PP_3851 0.79 -0.8 4,375,901 + PP_3851 0.79 +0.4 4,375,902 - PP_3851 0.79 -0.7 4,375,902 - PP_3851 0.79 -0.3 4,375,903 + PP_3851 0.80 +0.0 4,375,903 + +2.7 4,375,903 + PP_3851 0.80 +0.2 4,375,903 + PP_3851 0.80 +0.6 4,375,904 - PP_3851 0.80 -0.2 4,375,904 - PP_3851 0.80 -0.3 4,375,904 - PP_3851 0.80 -1.2 4,375,904 - PP_3851 0.80 -0.9 4,375,921 + PP_3851 0.85 -1.6 4,375,922 - PP_3851 0.85 +1.6 4,375,947 + -0.5 4,375,948 - +0.2 4,375,964 - -0.1 4,375,979 + -0.4 4,375,979 + +0.2 4,375,980 - -0.6 4,375,980 - -0.2 4,375,993 + -0.3 4,375,993 + +0.8 4,376,005 + +3.7 4,376,005 + +1.7 4,376,005 + +1.3 4,376,006 - -0.6 4,376,006 - +1.7 4,376,006 - -0.8 4,376,006 - +0.7 4,376,009 + +0.5 4,376,009 + -0.1 4,376,009 + +0.3 4,376,009 + +0.3 4,376,055 + +2.7 4,376,056 - -2.4 4,376,066 + -0.4 4,376,066 + -0.5 4,376,066 + -0.8 4,376,067 - +0.4 4,376,067 - -0.4 4,376,067 - +0.1 4,376,067 - -0.3 4,376,067 - +0.2 4,376,067 - +2.7 4,376,079 - +0.3 4,376,079 - +0.0 4,376,214 - +0.0 4,376,214 - +1.3 4,376,260 + PP_3852 0.12 +0.7 4,376,296 - PP_3852 0.14 -0.7 4,376,296 - PP_3852 0.14 -0.3 4,376,367 - PP_3852 0.19 -0.8 4,376,393 + PP_3852 0.21 +0.1 4,376,393 + PP_3852 0.21 -0.4 4,376,433 + PP_3852 0.23 +0.3 4,376,462 + PP_3852 0.25 +0.1 4,376,463 - PP_3852 0.25 +0.5 4,376,463 - PP_3852 0.25 +0.9 4,376,481 + PP_3852 0.27 -0.6 4,376,482 - PP_3852 0.27 -0.6 4,376,514 - PP_3852 0.29 +0.2 4,376,591 - PP_3852 0.34 +1.0 4,376,644 - PP_3852 0.38 +1.1 4,376,658 - PP_3852 0.39 +0.7 4,376,708 + PP_3852 0.42 +0.3 4,376,714 + PP_3852 0.42 +0.5 4,376,714 + +0.7 4,376,716 + PP_3852 0.42 -0.3 4,376,718 + PP_3852 0.43 +0.4 4,376,719 - PP_3852 0.43 +0.4 4,376,719 - PP_3852 0.43 +0.7 4,376,725 + PP_3852 0.43 +0.5 4,376,821 + PP_3852 0.50 -0.3 4,376,821 + PP_3852 0.50 -0.4 4,376,822 - PP_3852 0.50 +0.1 4,376,851 - PP_3852 0.52 -0.7 4,376,879 + PP_3852 0.53 -0.9 4,376,882 + PP_3852 0.54 +1.2 4,376,882 + PP_3852 0.54 +0.4 4,376,955 + PP_3852 0.59 +0.7 4,376,960 + PP_3852 0.59 -0.3 4,376,960 + PP_3852 0.59 -0.1 4,376,960 + PP_3852 0.59 +0.5 4,376,960 + PP_3852 0.59 -0.5 4,376,960 + PP_3852 0.59 +0.7 4,376,960 + PP_3852 0.59 -0.3 4,376,961 - PP_3852 0.59 -0.5 4,376,971 - PP_3852 0.60 -0.4 4,376,978 + PP_3852 0.60 +2.7 4,376,978 + PP_3852 0.60 -2.3 4,377,004 - PP_3852 0.62 +1.0 4,377,047 - PP_3852 0.65 -0.1 4,377,115 - PP_3852 0.69 +0.3 4,377,115 - PP_3852 0.69 -1.8 4,377,129 - PP_3852 0.70 +1.2 4,377,135 + PP_3852 0.71 -1.9 4,377,135 + PP_3852 0.71 -0.3 4,377,220 + PP_3852 0.76 +0.1 4,377,232 + PP_3852 0.77 +0.1 4,377,232 + PP_3852 0.77 +0.9 4,377,253 + PP_3852 0.79 -0.5 4,377,291 + PP_3852 0.81 +0.9 4,377,291 + PP_3852 0.81 +0.6 4,377,291 + PP_3852 0.81 +0.4 4,377,291 + PP_3852 0.81 -0.6 4,377,292 - PP_3852 0.81 +0.7 4,377,292 - PP_3852 0.81 +1.1 4,377,297 - PP_3852 0.82 +0.4 4,377,297 - PP_3852 0.82 -1.2 4,377,297 - PP_3852 0.82 +1.3 4,377,339 + PP_3852 0.84 +1.5 4,377,339 + PP_3852 0.84 +0.2 4,377,339 + PP_3852 0.84 +0.7 4,377,339 + PP_3852 0.84 +0.3 4,377,345 + PP_3852 0.85 +2.1 4,377,346 - PP_3852 0.85 -0.2 4,377,376 + PP_3852 0.87 +0.1 4,377,430 - -0.7 4,377,502 + +0.8 4,377,503 - -0.1 4,377,503 - -0.8 4,377,566 + +0.3 4,377,566 + -1.5 4,377,566 + -0.1 4,377,566 + +0.3 4,377,566 + -0.6 4,377,567 - +0.9 4,377,567 - +0.7 4,377,585 - -1.4 4,377,626 + -0.8 4,377,626 + -0.5 4,377,627 - +0.6 4,377,627 - +0.7 4,377,627 - +0.5 4,377,641 + -1.0 4,377,641 + +0.2 4,377,641 + +0.0 4,377,641 + +0.7 4,377,642 - -0.3 4,377,642 - -0.5 4,377,642 - +2.2 4,377,642 - -1.3 4,377,642 - +0.9 4,377,647 + +0.5 4,377,647 + -0.2 4,377,647 + -0.2 4,377,647 + +0.9 4,377,693 + -1.1 4,377,693 + -0.0 4,377,693 + -1.8 4,377,693 + +0.0 4,377,693 + +0.2 4,377,694 - +0.2 4,377,694 - -0.4 4,377,694 - -0.3 4,377,694 - -3.9 4,377,695 + -0.1 4,377,695 + +0.3 4,377,695 + -0.3 4,377,695 + -0.7 4,377,695 + -0.8 4,377,696 - -0.1 4,377,696 - +0.0 4,377,701 + PP_5638 0.10 -0.8 4,377,701 + PP_5638 0.10 -0.3 4,377,701 + PP_5638 0.10 -0.4 4,377,701 + PP_5638 0.10 -0.8 4,377,701 + PP_5638 0.10 +0.2 4,377,702 - PP_5638 0.11 -1.0 4,378,033 + PP_5638 0.73 +0.9 4,378,034 - PP_5638 0.73 +0.3 4,378,115 + PP_5638 0.88 -0.8 4,378,258 + PP_3854 0.16 -0.3 4,378,258 + PP_3854 0.16 -0.9 4,378,258 + PP_3854 0.16 +0.9 4,378,259 - PP_3854 0.16 +1.0 4,378,456 + PP_3854 0.52 +2.7 4,378,456 + PP_3854 0.52 +0.7 4,378,456 + PP_3854 0.52 -1.3 4,378,456 + PP_3854 0.52 +0.6 4,378,457 - PP_3854 0.53 -3.0 4,378,457 - PP_3854 0.53 +0.3 4,378,519 + PP_3854 0.64 +0.9 4,378,519 - PP_3854 0.64 -0.6 4,378,520 - PP_3854 0.64 -0.7 4,378,528 + PP_3854 0.66 -0.1 4,378,567 + PP_3854 0.73 +0.7 4,378,568 - PP_3854 0.73 -0.3
Or see this region's nucleotide sequence