Strain Fitness in Pseudomonas putida KT2440 around PP_3443

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
3,898,642	+		PP_3441	0.57	+2.7
3,898,643	-		PP_3441	0.57	+1.7
3,898,709	-		PP_3441	0.63	-0.5
3,898,807	+		PP_3441	0.71	+1.1
3,898,808	-		PP_3441	0.71	-0.5
3,898,808	-		PP_3441	0.71	-0.3
3,898,969	+		PP_3441	0.85	-0.5
3,898,969	+		PP_3441	0.85	-1.7
3,898,970	-		PP_3441	0.85	+0.3
3,898,970	-		PP_3441	0.85	-0.5
3,899,374	-				+1.1
3,899,375	+				-0.9
3,899,436	-				+0.5
3,899,588	+				-0.9
3,899,680	+				-2.6
3,899,680	+				-2.2
3,899,681	-				-0.6
3,899,777	+		PP_3443	0.11	+1.7
3,899,778	-		PP_3443	0.11	-0.2
3,899,837	+		PP_3443	0.15	-1.2
3,900,019	+		PP_3443	0.26	-1.9
3,900,020	-		PP_3443	0.26	-2.5
3,900,145	+		PP_3443	0.34	-2.6
3,900,146	-		PP_3443	0.34	+0.1
3,900,151	+		PP_3443	0.34	-1.9
3,900,151	+		PP_3443	0.34	+0.4
3,900,236	+		PP_3443	0.40	+0.6
3,900,304	+		PP_3443	0.44	-1.9
3,900,304	+		PP_3443	0.44	-0.9
3,900,304	+		PP_3443	0.44	-0.6
3,900,305	-		PP_3443	0.44	-0.2
3,900,305	-		PP_3443	0.44	-1.4
3,900,359	+		PP_3443	0.47	-0.7
3,900,360	-		PP_3443	0.47	-2.4
3,900,740	+		PP_3443	0.71	-0.7
3,900,741	-		PP_3443	0.71	-2.0
3,900,782	-		PP_3443	0.73	+0.5
3,900,885	-		PP_3443	0.80	-1.6
3,900,892	+		PP_3443	0.80	+0.3
3,900,892	+		PP_3443	0.80	-1.3
3,900,892	+		PP_3443	0.80	-1.2
3,900,893	-		PP_3443	0.80	-2.3
3,900,893	-		PP_3443	0.80	+0.1
3,901,033	+		PP_3443	0.89	-0.7
3,901,033	+		PP_3443	0.89	+0.4
3,901,034	-		PP_3443	0.89	-0.6
3,901,201	+				-2.9
3,901,202	-				-1.6
3,901,202	-				-0.2
3,901,202	-				-0.5
3,901,202	-				-0.1
3,901,223	+				-0.9
3,901,238	+				+1.4
3,901,276	+				-1.3
3,901,276	+				+1.3
3,901,277	-				-0.8
3,901,369	-				+0.3
3,901,407	+		PP_3444	0.17	+0.7
3,901,584	+		PP_3444	0.54	-0.3
3,901,584	+		PP_3444	0.54	-0.6
3,901,584	+		PP_3444	0.54	-1.4
3,901,584	+		PP_3444	0.54	+1.5
3,901,585	-		PP_3444	0.55	+0.4
3,901,642	+		PP_3444	0.66	-0.3
3,901,642	+		PP_3444	0.66	+1.2
3,901,643	-				+0.7
3,901,643	-		PP_3444	0.67	+0.0
3,901,643	-		PP_3444	0.67	-0.7
3,901,837	+				-0.7
3,901,838	-				-1.0
3,901,960	+		PP_3445	0.11	-1.6
3,901,960	+		PP_3445	0.11	+0.7
3,901,961	-				+0.7
3,901,976	-				+2.1
3,901,988	-				+1.1