Strain Fitness in Pseudomonas putida KT2440 around PP_3359

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
3,798,190	+		PP_3358	0.15	+0.8
3,798,391	+		PP_3358	0.39	+0.4
3,798,391	+		PP_3358	0.39	+0.3
3,798,391	+		PP_3358	0.39	-0.7
3,798,391	+		PP_3358	0.39	-1.1
3,798,391	+		PP_3358	0.39	+0.3
3,798,391	+		PP_3358	0.39	+1.4
3,798,391	+		PP_3358	0.39	-1.7
3,798,391	+		PP_3358	0.39	-0.3
3,798,391	+		PP_3358	0.39	+0.0
3,798,392	-		PP_3358	0.39	+1.4
3,798,392	-		PP_3358	0.39	-1.6
3,798,394	+		PP_3358	0.39	-1.7
3,798,395	-		PP_3358	0.39	-0.3
3,798,586	+		PP_3358	0.62	+0.7
3,798,587	-		PP_3358	0.62	+0.2
3,798,726	+		PP_3358	0.79	+0.1
3,798,889	+				+0.7
3,798,890	-				-0.1
3,798,890	-				+2.0
3,798,890	-				+0.2
3,799,056	+				+0.2
3,799,056	+				-0.2
3,799,070	-				+0.8
3,799,102	+				-0.5
3,799,102	+				+2.7
3,799,102	+				-1.4
3,799,115	-				+0.0
3,799,219	+		PP_3359	0.26	-0.4
3,799,219	+		PP_3359	0.26	-1.6
3,799,220	-		PP_3359	0.26	-0.9
3,799,662	-				+0.8
3,799,662	-				-1.6
3,799,844	+		PP_3360	0.16	-0.3
3,800,124	-		PP_3360	0.41	+0.0
3,800,221	-		PP_3360	0.49	+0.3
3,800,413	+		PP_3360	0.66	+0.2
3,800,413	+		PP_3360	0.66	-0.5
3,800,504	+		PP_3360	0.74	+0.1
3,800,504	+		PP_3360	0.74	-0.7
3,800,505	-		PP_3360	0.75	+0.4