Strain Fitness in Pseudomonas putida KT2440 around PP_3314

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
3,748,932	+				+0.2
3,748,933	-				-0.0
3,748,933	-				+0.2
3,748,933	-				-0.5
3,748,933	-				+1.1
3,748,933	-				+0.1
3,749,177	-		PP_3312	0.57	+0.2
3,749,223	-		PP_3312	0.69	-1.2
3,749,447	+				+0.0
3,749,447	+				-0.8
3,749,564	-		PP_3313	0.21	+0.1
3,749,645	+		PP_3313	0.43	+1.7
3,749,896	+				+1.4
3,749,897	-				+1.4
3,749,898	+				+0.5
3,749,898	+				-0.4
3,749,899	-				+0.1
3,749,899	-				+0.6
3,749,899	-				-0.5
3,749,899	-				-1.1
3,749,899	-				+0.8
3,750,026	+		PP_3314	0.31	-1.6
3,750,100	+		PP_3314	0.47	-1.8
3,750,162	-		PP_3314	0.61	-0.7
3,750,162	-		PP_3314	0.61	-0.2
3,750,162	-		PP_3314	0.61	-2.2
3,750,321	+				+2.0
3,750,321	+				-0.3
3,750,322	-				+0.9
3,750,322	-				+0.4
3,750,322	-				+0.6
3,750,335	+				-0.1
3,750,335	+				-1.5
3,750,336	-				+1.1
3,750,421	-				+1.1
3,750,421	-				+1.6
3,750,471	+				-0.7
3,750,472	-				+0.7
3,750,472	-				-1.2
3,750,530	+				+1.1
3,750,531	-				+0.1
3,750,841	+		PP_3315	0.66	+0.4
3,750,841	+		PP_3315	0.66	+0.5
3,750,856	+		PP_3315	0.69	-0.6
3,750,856	+		PP_3315	0.69	-0.2
3,750,929	+		PP_3315	0.85	-0.3
3,751,259	+				+1.0