Strain Fitness in Pseudomonas putida KT2440 around PP_3185

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
3,613,312	+		PP_3184	0.89	-0.2
3,613,368	+				-2.4
3,613,368	+				+0.7
3,613,369	-				+0.8
3,613,369	-				+0.8
3,613,369	-				-0.4
3,613,374	-				-0.8
3,613,374	-				+0.4
3,613,374	-				+0.1
3,613,374	-				-1.9
3,613,535	+				+1.7
3,613,689	+				-0.4
3,613,765	-				+0.5
3,613,765	-				+1.1
3,613,765	-				-0.9
3,613,765	-				-0.5
3,613,776	+				+0.4
3,613,776	+				+0.8
3,613,776	+				-0.4
3,613,777	-				-0.0
3,613,798	-				+0.4
3,613,902	+				-1.9
3,613,927	+				-0.0
3,613,929	+				+0.1
3,613,930	-				+1.2
3,613,930	-				+0.0
3,613,984	-				-0.3
3,614,386	+		PP_3185	0.40	-0.4
3,614,386	+		PP_3185	0.40	-0.9
3,614,506	+		PP_3185	0.58	-0.8
3,614,507	-		PP_3185	0.58	-0.6
3,614,548	+		PP_3185	0.65	-1.5
3,614,614	-		PP_3185	0.75	-0.7
3,614,829	+				-0.2
3,614,829	+				+0.2
3,614,829	+				+0.4
3,614,833	+				-1.3
3,614,833	+				+0.4
3,614,833	+				+1.1
3,614,834	-				-1.3
3,614,834	-				-1.1
3,614,895	+		PP_3186	0.18	-0.5
3,614,895	+				+2.1
3,614,895	+		PP_3186	0.18	-0.1
3,614,896	-		PP_3186	0.18	-0.8
3,614,896	-		PP_3186	0.18	+0.2
3,614,911	-		PP_3186	0.20	-0.6
3,614,911	-		PP_3186	0.20	+0.5
3,614,911	-		PP_3186	0.20	-0.2
3,614,911	-		PP_3186	0.20	+0.1
3,615,109	-		PP_3186	0.50	-0.3
3,615,109	-		PP_3186	0.50	-1.4
3,615,109	-		PP_3186	0.50	+0.0
3,615,109	-		PP_3186	0.50	+2.7
3,615,109	-		PP_3186	0.50	+0.8
3,615,267	+		PP_3186	0.74	-0.5
3,615,268	-		PP_3186	0.74	-0.7
3,615,436	-				-0.1
3,615,458	+				+0.3
3,615,459	-				-1.1
3,615,459	-				-1.2
3,615,459	-				+1.1
3,615,459	-				+0.8
3,615,502	+				-0.6
3,615,542	+				-0.1
3,615,542	+				-0.2
3,615,543	-				-1.4
3,615,543	-				+0.2