Strain Fitness in Pseudomonas putida KT2440 around PP_2846
Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
Position | Strand | Gene | LocusTag | Fraction | 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. |
---|---|---|---|---|---|
remove | |||||
3,249,196 | + | PP_2845 | 0.48 | -0.5 | |
3,249,291 | - | PP_2845 | 0.53 | +0.4 | |
3,249,291 | - | PP_2845 | 0.53 | -0.6 | |
3,249,560 | + | PP_2845 | 0.69 | +0.8 | |
3,249,588 | - | PP_2845 | 0.71 | -1.6 | |
3,249,599 | + | PP_2845 | 0.71 | -0.1 | |
3,249,600 | - | PP_2845 | 0.72 | +0.4 | |
3,249,798 | - | PP_2845 | 0.83 | +0.4 | |
3,249,940 | - | +0.4 | |||
3,250,001 | + | -0.2 | |||
3,250,002 | - | +0.6 | |||
3,250,114 | + | +0.5 | |||
3,250,114 | + | +0.4 | |||
3,250,114 | + | -4.7 | |||
3,250,115 | - | -0.4 | |||
3,250,117 | - | +0.4 | |||
3,250,473 | + | PP_2846 | 0.75 | -0.3 | |
3,250,473 | + | PP_2846 | 0.75 | -0.2 | |
3,250,473 | + | PP_2846 | 0.75 | -0.8 | |
3,250,515 | - | PP_2846 | 0.84 | -3.4 | |
3,250,575 | + | -0.3 | |||
3,250,990 | - | PP_2847 | 0.68 | +0.4 | |
3,250,990 | - | PP_2847 | 0.68 | -1.5 | |
3,251,229 | + | PP_2848 | 0.10 | -0.7 | |
3,251,230 | - | PP_2848 | 0.10 | -0.1 | |
3,251,362 | + | PP_2848 | 0.30 | -1.3 | |
3,251,379 | + | +1.2 |
Or see this region's nucleotide sequence