Strain Fitness in Pseudomonas putida KT2440 around PP_2203
Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
Position | Strand | Gene | LocusTag | Fraction | 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. |
---|---|---|---|---|---|
remove | |||||
2,507,713 | + | PP_2201 | 0.54 | +0.1 | |
2,507,714 | - | PP_2201 | 0.54 | -0.7 | |
2,507,833 | + | PP_2201 | 0.66 | +0.8 | |
2,508,013 | + | PP_2201 | 0.84 | +0.7 | |
2,508,100 | + | -0.6 | |||
2,508,100 | + | -0.3 | |||
2,508,100 | + | -0.4 | |||
2,508,276 | - | -0.7 | |||
2,508,276 | - | -0.3 | |||
2,508,559 | - | PP_2202 | 0.71 | -1.5 | |
2,508,676 | - | -1.1 | |||
2,508,690 | + | +0.7 | |||
2,508,690 | + | -1.2 | |||
2,508,691 | - | +0.6 | |||
2,508,691 | - | -1.7 | |||
2,508,691 | - | +0.6 | |||
2,508,741 | + | PP_2203 | 0.13 | +1.7 | |
2,508,741 | + | PP_2203 | 0.13 | +0.7 | |
2,508,741 | + | PP_2203 | 0.13 | -1.8 | |
2,509,131 | - | -0.9 | |||
2,509,241 | + | PP_2204 | 0.13 | -0.8 | |
2,509,242 | - | PP_2204 | 0.13 | -1.1 | |
2,509,269 | - | PP_2204 | 0.16 | -1.8 | |
2,509,340 | + | PP_2204 | 0.24 | -0.3 | |
2,509,369 | + | PP_2204 | 0.27 | +0.4 | |
2,509,392 | + | PP_2204 | 0.30 | +1.4 | |
2,509,393 | - | PP_2204 | 0.30 | +0.8 | |
2,509,397 | + | PP_2204 | 0.30 | -0.2 | |
2,509,442 | + | PP_2204 | 0.35 | -0.7 | |
2,509,657 | - | PP_2204 | 0.60 | +0.4 |
Or see this region's nucleotide sequence