Strain Fitness in Pseudomonas putida KT2440 around PP_2192

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
2,495,843	+				-0.8
2,495,843	+				+1.7
2,495,844	-				-1.5
2,495,844	-				-0.8
2,495,844	-				+1.1
2,495,848	+				-0.9
2,495,873	+				+0.4
2,495,874	-				-1.4
2,495,874	-				-0.3
2,496,081	+		PP_2190	0.76	-0.9
2,496,168	+				+0.6
2,496,168	+				-0.4
2,496,168	+				-0.2
2,496,169	-				+1.0
2,496,169	-				-0.2
2,496,255	+		PP_2191	0.19	-0.5
2,496,255	+		PP_2191	0.19	+0.8
2,496,255	+		PP_2191	0.19	-0.1
2,496,256	-		PP_2191	0.19	-0.2
2,496,256	-		PP_2191	0.19	+0.2
2,496,367	+		PP_2191	0.42	-0.8
2,496,367	+		PP_2191	0.42	+0.6
2,496,367	+		PP_2191	0.42	+0.5
2,496,368	-		PP_2191	0.42	+0.6
2,496,368	-		PP_2191	0.42	-0.1
2,496,441	+		PP_2191	0.58	+1.5
2,496,627	+				-0.2
2,496,629	+				-0.7
2,496,630	-				-0.4
2,496,813	-				-2.2
2,496,823	+				-0.2
2,496,824	-				+0.2
2,496,824	-				-0.2
2,496,899	-		PP_2192	0.11	-0.7
2,497,237	+		PP_2192	0.41	-1.3
2,497,238	-		PP_2192	0.41	-1.0
2,497,238	-		PP_2192	0.41	-1.2
2,497,238	-		PP_2192	0.41	-0.6
2,497,240	-		PP_2192	0.41	+1.1
2,497,360	+		PP_2192	0.52	+0.9
2,497,393	-		PP_2192	0.55	-1.0
2,497,475	+		PP_2192	0.62	-0.3
2,497,476	-		PP_2192	0.62	-1.4
2,497,852	+				+0.5
2,497,870	+				+0.3
2,497,871	-				-0.4
2,497,871	-				-1.4
2,497,976	+				-0.2
2,498,094	+				-2.6
2,498,095	-				-2.4
2,498,343	+				-0.3
2,498,394	+				+2.7
2,498,394	+		PP_2193	0.12	+0.9
2,498,395	-		PP_2193	0.12	-1.5
2,498,395	-		PP_2193	0.12	-0.8
2,498,395	-		PP_2193	0.12	+0.2
2,498,395	-		PP_2193	0.12	+0.3
2,498,395	-		PP_2193	0.12	-1.0
2,498,534	-		PP_2193	0.18	+0.3
2,498,695	-		PP_2193	0.24	-2.2