Strain Fitness in Pseudomonas putida KT2440 around PP_2001

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
2,268,364	-		PP_2000	0.46	+1.7
2,269,170	+				-0.4
2,269,333	+		PP_2001	0.11	-0.9
2,269,333	+		PP_2001	0.11	-1.9
2,269,334	-		PP_2001	0.11	+0.1
2,269,406	-		PP_2001	0.17	-1.8
2,269,477	-		PP_2001	0.23	-0.4
2,269,477	-		PP_2001	0.23	-2.2
2,269,591	+		PP_2001	0.32	-2.9
2,269,591	+		PP_2001	0.32	-3.4
2,269,591	+		PP_2001	0.32	-0.9
2,269,592	-		PP_2001	0.33	-1.3
2,269,592	-		PP_2001	0.33	-2.1
2,269,592	-		PP_2001	0.33	-3.5
2,269,592	-		PP_2001	0.33	-3.4
2,269,610	+		PP_2001	0.34	-2.4
2,269,611	-		PP_2001	0.34	-2.4
2,269,611	-		PP_2001	0.34	+0.1
2,269,621	+		PP_2001	0.35	-2.1
2,269,725	+		PP_2001	0.43	-3.6
2,269,726	-		PP_2001	0.44	-2.6
2,269,903	-		PP_2001	0.58	-0.1
2,270,010	+		PP_2001	0.67	-1.2
2,270,011	-		PP_2001	0.67	-1.7
2,270,011	-		PP_2001	0.67	-1.1
2,270,086	+		PP_2001	0.73	-0.6
2,270,087	-		PP_2001	0.73	-0.3
2,270,319	+				-2.9
2,270,805	+		PP_2002	0.52	-0.6
2,270,806	-		PP_2002	0.52	+1.5
2,270,847	-		PP_2002	0.57	-1.4
2,270,884	-		PP_2002	0.62	+0.2
2,270,884	-		PP_2002	0.62	-0.7
2,271,171	+				-1.3
2,271,171	+				-0.6
2,271,171	+				-0.2
2,271,171	+				-0.9
2,271,171	+				+0.1
2,271,171	+				-1.2
2,271,171	+				+0.8
2,271,171	+				+0.5
2,271,172	-				+0.9
2,271,172	-				-0.3
2,271,172	-				+2.7
2,271,172	-				-0.1
2,271,172	-				+0.1
2,271,172	-				-0.1
2,271,172	-				+0.1
2,271,172	-				+2.7
2,271,173	+				+0.3
2,271,173	+				+2.1
2,271,174	-				+1.0
2,271,227	+				+0.2
2,271,228	-				+1.7
2,271,236	+				-0.2
2,271,249	+				+0.7
2,271,249	+				+0.3
2,271,250	-				-1.1