Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1827 and PP_1828 are separated by 49 nucleotides PP_1828 and PP_1829 are separated by 65 nucleotides PP_1829 and PP_1830 are separated by 234 nucleotides
PP_1827: PP_1827 - N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase, at 2,051,860 to 2,052,768
_1827
PP_1828: PP_1828 - conserved protein of unknown function, at 2,052,818 to 2,053,600
_1828
PP_1829: PP_1829 - Hydrolase, alpha/beta fold family, at 2,053,666 to 2,054,625
_1829
PP_1830: PP_1830 - Chorismate synthase, at 2,054,860 to 2,055,951
_1830
Position (kb)
2053
2054
2055 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2052.772 kb on - strand at 2052.881 kb on - strand at 2052.915 kb on + strand, within PP_1828 at 2052.915 kb on + strand, within PP_1828 at 2053.075 kb on + strand, within PP_1828 at 2053.434 kb on + strand, within PP_1828 at 2053.435 kb on - strand, within PP_1828 at 2053.502 kb on + strand, within PP_1828 at 2053.618 kb on + strand at 2053.618 kb on + strand at 2053.619 kb on - strand at 2053.619 kb on - strand at 2053.619 kb on - strand at 2053.619 kb on - strand at 2053.619 kb on - strand at 2053.745 kb on + strand at 2053.746 kb on - strand at 2053.746 kb on - strand at 2053.746 kb on - strand at 2053.746 kb on - strand at 2054.276 kb on + strand, within PP_1829 at 2054.276 kb on + strand, within PP_1829 at 2054.277 kb on - strand, within PP_1829 at 2054.557 kb on + strand at 2054.558 kb on - strand at 2054.558 kb on - strand at 2054.558 kb on - strand at 2054.634 kb on - strand at 2054.636 kb on + strand at 2054.636 kb on + strand at 2054.636 kb on + strand at 2054.636 kb on + strand at 2054.636 kb on + strand at 2054.636 kb on + strand at 2054.637 kb on - strand at 2054.637 kb on - strand at 2054.681 kb on + strand at 2054.681 kb on + strand at 2054.681 kb on + strand at 2054.681 kb on + strand at 2054.681 kb on + strand at 2054.681 kb on + strand at 2054.681 kb on + strand at 2054.682 kb on - strand at 2054.682 kb on - strand at 2054.682 kb on - strand at 2054.682 kb on - strand at 2054.682 kb on - strand at 2054.682 kb on - strand at 2054.814 kb on + strand at 2054.814 kb on + strand at 2054.814 kb on + strand at 2054.814 kb on + strand at 2054.814 kb on + strand at 2054.814 kb on + strand at 2054.814 kb on + strand at 2054.814 kb on + strand at 2054.814 kb on + strand at 2054.814 kb on + strand at 2054.814 kb on + strand at 2054.815 kb on - strand at 2054.815 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 2,052,772 - +0.1 2,052,881 - +0.4 2,052,915 + PP_1828 0.12 +0.8 2,052,915 + PP_1828 0.12 -1.0 2,053,075 + PP_1828 0.33 -0.8 2,053,434 + PP_1828 0.79 +0.2 2,053,435 - PP_1828 0.79 +0.3 2,053,502 + PP_1828 0.87 -0.2 2,053,618 + -0.4 2,053,618 + +0.3 2,053,619 - -1.7 2,053,619 - +1.7 2,053,619 - -0.1 2,053,619 - -0.8 2,053,619 - +0.1 2,053,745 + +1.1 2,053,746 - +0.7 2,053,746 - +0.5 2,053,746 - -0.1 2,053,746 - +0.4 2,054,276 + PP_1829 0.64 -1.4 2,054,276 + PP_1829 0.64 -2.1 2,054,277 - PP_1829 0.64 +0.1 2,054,557 + +0.7 2,054,558 - +1.4 2,054,558 - +0.8 2,054,558 - -0.3 2,054,634 - +1.3 2,054,636 + +0.2 2,054,636 + +0.4 2,054,636 + -0.0 2,054,636 + -0.5 2,054,636 + +0.4 2,054,636 + -0.9 2,054,637 - +0.6 2,054,637 - -1.7 2,054,681 + -0.6 2,054,681 + +0.4 2,054,681 + +0.8 2,054,681 + +0.5 2,054,681 + -0.8 2,054,681 + -0.7 2,054,681 + +0.1 2,054,682 - -0.7 2,054,682 - +0.7 2,054,682 - +0.1 2,054,682 - +0.5 2,054,682 - -1.7 2,054,682 - -1.4 2,054,814 + -0.1 2,054,814 + -0.1 2,054,814 + -0.7 2,054,814 + -0.9 2,054,814 + +0.9 2,054,814 + -0.3 2,054,814 + -0.6 2,054,814 + -0.5 2,054,814 + +4.2 2,054,814 + -0.9 2,054,814 + +1.2 2,054,815 - -1.1 2,054,815 - +1.1
Or see this region's nucleotide sequence