Strain Fitness in Pseudomonas putida KT2440 around PP_1512

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1510 and PP_1511 overlap by 1 nucleotidesPP_1511 and PP_1512 are separated by 2 nucleotidesPP_1512 and PP_1513 are separated by 104 nucleotides PP_1510: PP_1510 - putative Carboxyvinyl-carboxyphosphonate phosphorylmutase, at 1,715,055 to 1,715,885 _1510 PP_1511: PP_1511 - conserved protein of unknown function, at 1,715,885 to 1,716,574 _1511 PP_1512: PP_1512 - conserved protein of unknown function, at 1,716,577 to 1,717,197 _1512 PP_1513: PP_1513 - Ribosomal RNA small subunit methyltransferase J, at 1,717,302 to 1,718,078 _1513 Position (kb) 1716 1717 1718Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1715.580 kb on + strand, within PP_1510at 1715.581 kb on - strand, within PP_1510at 1715.581 kb on - strand, within PP_1510at 1715.608 kb on - strand, within PP_1510at 1715.790 kb on + strand, within PP_1510at 1715.791 kb on - strand, within PP_1510at 1715.994 kb on + strand, within PP_1511at 1715.994 kb on + strand, within PP_1511at 1715.995 kb on - strand, within PP_1511at 1716.040 kb on - strand, within PP_1511at 1716.144 kb on + strand, within PP_1511at 1716.145 kb on - strand, within PP_1511at 1716.215 kb on + strand, within PP_1511at 1716.320 kb on + strand, within PP_1511at 1716.512 kb on + strandat 1717.023 kb on - strand, within PP_1512at 1717.023 kb on - strand, within PP_1512at 1717.023 kb on - strand, within PP_1512at 1717.091 kb on - strand, within PP_1512at 1717.145 kb on + strandat 1717.146 kb on - strandat 1717.166 kb on + strandat 1717.166 kb on + strandat 1717.167 kb on - strandat 1717.167 kb on - strandat 1717.167 kb on - strandat 1717.188 kb on + strandat 1717.189 kb on - strandat 1717.189 kb on - strandat 1717.189 kb on - strandat 1717.270 kb on - strandat 1717.351 kb on - strandat 1717.351 kb on - strandat 1717.594 kb on + strand, within PP_1513at 1717.825 kb on - strand, within PP_1513at 1717.825 kb on - strand, within PP_1513at 1718.019 kb on - strandat 1718.031 kb on - strandat 1718.044 kb on - strandat 1718.052 kb on + strandat 1718.052 kb on + strandat 1718.053 kb on - strandat 1718.087 kb on + strandat 1718.088 kb on - strandat 1718.110 kb on + strandat 1718.110 kb on + strandat 1718.110 kb on + strandat 1718.111 kb on - strandat 1718.111 kb on - strandat 1718.111 kb on - strandat 1718.111 kb on - strandat 1718.176 kb on + strandat 1718.188 kb on + strandat 1718.188 kb on + strandat 1718.189 kb on - strandat 1718.189 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
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1,715,580 + PP_1510 0.63 +0.1
1,715,581 - PP_1510 0.63 +1.4
1,715,581 - PP_1510 0.63 -0.3
1,715,608 - PP_1510 0.67 -0.3
1,715,790 + PP_1510 0.88 -0.3
1,715,791 - PP_1510 0.89 -0.2
1,715,994 + PP_1511 0.16 +0.2
1,715,994 + PP_1511 0.16 +0.1
1,715,995 - PP_1511 0.16 -1.1
1,716,040 - PP_1511 0.22 +0.0
1,716,144 + PP_1511 0.38 +0.8
1,716,145 - PP_1511 0.38 +0.4
1,716,215 + PP_1511 0.48 +0.7
1,716,320 + PP_1511 0.63 -0.2
1,716,512 + -0.2
1,717,023 - PP_1512 0.72 -2.1
1,717,023 - PP_1512 0.72 -1.1
1,717,023 - PP_1512 0.72 -1.0
1,717,091 - PP_1512 0.83 +0.0
1,717,145 + -0.2
1,717,146 - -2.4
1,717,166 + -0.1
1,717,166 + +0.3
1,717,167 - -0.8
1,717,167 - -0.3
1,717,167 - +0.5
1,717,188 + -0.5
1,717,189 - +0.5
1,717,189 - -0.1
1,717,189 - +2.7
1,717,270 - +2.7
1,717,351 - +0.1
1,717,351 - -0.5
1,717,594 + PP_1513 0.38 +1.1
1,717,825 - PP_1513 0.67 -1.1
1,717,825 - PP_1513 0.67 -1.3
1,718,019 - +0.1
1,718,031 - +0.4
1,718,044 - -1.1
1,718,052 + -0.7
1,718,052 + +0.6
1,718,053 - +0.6
1,718,087 + -0.9
1,718,088 - +0.7
1,718,110 + -0.6
1,718,110 + +0.2
1,718,110 + +3.7
1,718,111 - +0.3
1,718,111 - +0.1
1,718,111 - -1.6
1,718,111 - -0.7
1,718,176 + -0.7
1,718,188 + -0.1
1,718,188 + +0.6
1,718,189 - +0.2
1,718,189 - -1.4

Or see this region's nucleotide sequence