Strain Fitness in Pseudomonas putida KT2440 around PP_1451

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
1,657,884	-		PP_1450	0.64	+0.7
1,657,934	+		PP_1450	0.67	-0.0
1,657,984	+				+2.7
1,657,985	-		PP_1450	0.70	+0.6
1,658,104	+		PP_1450	0.77	+0.7
1,658,104	+		PP_1450	0.77	-0.8
1,658,104	+		PP_1450	0.77	+0.4
1,658,105	-		PP_1450	0.77	+0.2
1,658,105	-		PP_1450	0.77	-0.4
1,658,105	-		PP_1450	0.77	-0.0
1,658,105	-		PP_1450	0.77	-0.3
1,658,105	-		PP_1450	0.77	-0.8
1,658,105	-		PP_1450	0.77	-0.3
1,658,105	-		PP_1450	0.77	-0.4
1,658,108	+		PP_1450	0.77	+0.5
1,658,108	+		PP_1450	0.77	+0.9
1,658,109	-		PP_1450	0.77	-0.1
1,658,109	-		PP_1450	0.77	+0.7
1,658,109	-		PP_1450	0.77	-0.3
1,658,109	-		PP_1450	0.77	+1.7
1,658,305	+		PP_1450	0.89	-1.2
1,658,307	+				+1.2
1,658,308	-		PP_1450	0.89	+0.2
1,658,308	-		PP_1450	0.89	+0.9
1,658,528	-				-0.5
1,658,568	+				-1.2
1,658,568	+				-2.4
1,658,568	+				-1.0
1,658,569	-				-1.0
1,658,569	-				-0.8
1,658,569	-				+0.5
1,658,569	-				+0.5
1,658,613	+				-1.7
1,658,613	+				+0.3
1,658,613	+				+2.7
1,658,613	+				+0.8
1,658,614	-				-0.7
1,658,635	+				-0.7
1,658,635	+				-0.8
1,658,635	+				+0.7
1,658,636	-				-0.1
1,658,636	-				-0.7
1,658,637	+				+0.3
1,658,748	+		PP_1451	0.20	-0.4
1,658,749	-		PP_1451	0.20	-0.9
1,658,749	-		PP_1451	0.20	-0.5
1,658,853	+		PP_1451	0.39	-2.2
1,658,910	+		PP_1451	0.49	-0.5
1,658,986	-		PP_1451	0.63	-1.4
1,659,195	+				-1.0
1,659,195	+				-0.8
1,659,195	+				+0.3
1,659,195	+				+0.4
1,659,195	+				-0.1
1,659,195	+				+0.3
1,659,196	-				+0.7
1,659,196	-				+0.4
1,659,196	-				+1.4
1,659,196	-				-1.3
1,659,196	-				-0.2
1,659,202	-				-0.7
1,659,217	+				-0.2
1,659,217	+				-0.4
1,659,217	+				+0.6
1,659,218	-				+0.5
1,659,218	-				+0.2
1,659,250	+				-1.5
1,659,250	+				-1.8
1,659,251	-				-0.7
1,659,251	-				+0.4
1,659,251	-				-1.7
1,659,251	-				-0.0
1,659,251	-				-0.1
1,659,261	+				-1.1
1,659,292	+		PP_1452	0.14	-1.2
1,659,292	+		PP_1452	0.14	+0.5
1,659,293	-		PP_1452	0.14	+0.2
1,659,295	+		PP_1452	0.14	-0.6
1,659,295	+		PP_1452	0.14	-2.1
1,659,295	+		PP_1452	0.14	+0.2
1,659,296	-		PP_1452	0.14	+1.0
1,659,296	-		PP_1452	0.14	-0.7
1,659,303	+		PP_1452	0.16	+0.3
1,659,303	+		PP_1452	0.16	-0.3
1,659,303	+		PP_1452	0.16	-2.0
1,659,303	+		PP_1452	0.16	+0.9
1,659,303	+		PP_1452	0.16	+0.6
1,659,304	-		PP_1452	0.16	+0.3
1,659,304	-		PP_1452	0.16	+0.3
1,659,345	+		PP_1452	0.23	+0.7
1,659,346	-		PP_1452	0.24	-1.2
1,659,470	-		PP_1452	0.46	+1.0
1,659,470	-		PP_1452	0.46	+0.3
1,659,639	+		PP_1452	0.77	-0.4
1,659,640	-		PP_1452	0.77	+0.4
1,659,697	+				+2.7
1,659,698	-		PP_1452	0.87	-0.5
1,659,791	+				-0.9
1,659,791	+				+0.4
1,659,877	+				-0.6
1,659,878	-				-0.4
1,659,904	+				+0.2
1,660,041	-		PP_1453	0.25	-0.4
1,660,058	-		PP_1453	0.28	+0.3
1,660,193	-		PP_1453	0.50	+0.0