Strain Fitness in Pseudomonas putida KT2440 around PP_1303

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
1,488,512	+				-1.3
1,488,560	-		PP_1302	0.15	+2.3
1,488,645	-		PP_1302	0.27	+0.5
1,488,713	-		PP_1302	0.36	+1.1
1,488,718	+		PP_1302	0.36	-0.4
1,488,718	+		PP_1302	0.36	-0.6
1,488,718	+		PP_1302	0.36	+0.5
1,488,719	-		PP_1302	0.36	+1.1
1,488,719	-		PP_1302	0.36	-0.6
1,488,719	-		PP_1302	0.36	+0.1
1,488,719	-		PP_1302	0.36	+2.7
1,488,719	-		PP_1302	0.36	+1.1
1,488,720	+		PP_1302	0.36	+2.1
1,488,720	+		PP_1302	0.36	+0.5
1,488,720	+		PP_1302	0.36	-2.9
1,488,720	+		PP_1302	0.36	-1.0
1,488,720	+		PP_1302	0.36	-0.4
1,488,721	-		PP_1302	0.37	-0.3
1,488,721	-		PP_1302	0.37	+0.0
1,488,721	-		PP_1302	0.37	+0.2
1,488,721	-		PP_1302	0.37	+0.8
1,488,721	-		PP_1302	0.37	+0.6
1,488,721	-		PP_1302	0.37	+0.3
1,488,721	-		PP_1302	0.37	-1.2
1,488,820	-		PP_1302	0.50	+1.7
1,488,989	+		PP_1302	0.72	-0.0
1,488,989	+		PP_1302	0.72	-0.3
1,488,989	+		PP_1302	0.72	-0.1
1,488,989	+		PP_1302	0.72	-1.5
1,488,990	-		PP_1302	0.72	-0.5
1,488,990	-		PP_1302	0.72	+0.5
1,488,990	-		PP_1302	0.72	+0.3
1,488,991	+		PP_1302	0.72	+0.6
1,489,025	+		PP_1302	0.77	+0.5
1,489,055	+		PP_1302	0.81	+1.0
1,489,056	-		PP_1302	0.81	-0.4
1,489,142	+				-1.2
1,489,232	+				-0.3
1,489,232	+				+1.0
1,489,233	-				+1.0
1,489,233	-				+0.9
1,489,233	-				+1.2
1,489,288	-				-0.6
1,489,320	+				-0.2
1,489,320	+				-1.5
1,489,321	-				+1.7
1,489,321	-				-0.9
1,489,324	+				+1.3
1,489,325	-				+1.5
1,489,325	-				-0.9
1,489,342	+				-0.8
1,489,380	+				-1.1
1,489,380	+				-1.0
1,489,380	+				-1.7
1,489,381	-				-0.2
1,489,381	-				-2.2
1,489,381	-				-1.4
1,489,381	-				-2.2
1,489,399	+				-1.4
1,489,399	+				-1.1
1,489,617	+		PP_1303	0.23	-1.3
1,489,618	-		PP_1303	0.23	-1.0
1,489,618	-		PP_1303	0.23	-0.4
1,489,698	-		PP_1303	0.32	-0.5
1,489,698	-		PP_1303	0.32	-0.7
1,489,846	-		PP_1303	0.48	+0.5
1,489,912	-		PP_1303	0.55	-0.5
1,489,989	+		PP_1303	0.64	-1.2
1,489,989	+		PP_1303	0.64	+0.7
1,489,989	+		PP_1303	0.64	+1.7
1,489,990	-		PP_1303	0.64	-0.7
1,489,995	+		PP_1303	0.64	-1.3
1,489,995	+		PP_1303	0.64	+0.3
1,489,995	+		PP_1303	0.64	-1.2
1,489,995	+		PP_1303	0.64	+1.1
1,489,996	-		PP_1303	0.64	-1.5
1,489,996	-		PP_1303	0.64	-2.3
1,489,996	-		PP_1303	0.64	-2.4
1,490,026	-		PP_1303	0.68	-0.6
1,490,026	-		PP_1303	0.68	-1.3
1,490,313	+				-0.8
1,490,313	+				-1.1
1,490,314	-				-0.4
1,490,319	+				-0.1
1,490,333	-				-0.5
1,490,370	+				-1.0
1,490,371	-				-0.4
1,490,371	-				+3.7
1,490,381	-				-0.1
1,490,400	+				-0.1
1,490,400	+				-0.7
1,490,400	+				-1.8
1,490,400	+				+1.7
1,490,401	-				-0.4
1,490,401	-				+2.7
1,490,491	+				+0.1
1,490,491	+				+0.4
1,490,573	+		PP_1304	0.12	-2.7
1,490,573	+		PP_1304	0.12	-0.2
1,490,574	-		PP_1304	0.12	-0.9
1,490,574	-		PP_1304	0.12	+0.0
1,490,574	-		PP_1304	0.12	-1.8
1,490,574	-		PP_1304	0.12	-2.3
1,490,629	+		PP_1304	0.15	-0.3
1,490,687	-		PP_1304	0.18	-1.2
1,490,687	-		PP_1304	0.18	-1.4
1,490,687	-		PP_1304	0.18	-1.4
1,490,753	-		PP_1304	0.22	-1.6
1,490,785	+		PP_1304	0.24	-1.1
1,490,998	+		PP_1304	0.35	-1.1
1,491,085	+		PP_1304	0.39	-0.2
1,491,086	-		PP_1304	0.39	+1.7
1,491,214	+		PP_1304	0.46	-0.2
1,491,214	+		PP_1304	0.46	-0.3
1,491,214	+		PP_1304	0.46	-1.8
1,491,215	-		PP_1304	0.46	+0.9
1,491,215	-		PP_1304	0.46	-0.1
1,491,305	+		PP_1304	0.51	-1.9
1,491,305	+		PP_1304	0.51	-1.5
1,491,306	-		PP_1304	0.51	-0.4
1,491,306	-		PP_1304	0.51	+1.7