Strain Fitness in Pseudomonas putida KT2440 around PP_1301

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
1,486,152	+				-0.9
1,486,153	-				+0.8
1,486,153	-				-0.3
1,486,163	-				+0.4
1,486,163	-				+0.3
1,486,289	+				-0.6
1,486,617	-		PP_1300	0.52	-0.3
1,486,617	-		PP_1300	0.52	+0.1
1,486,655	+		PP_1300	0.57	-0.3
1,486,656	-		PP_1300	0.57	-0.7
1,486,656	-		PP_1300	0.57	-0.2
1,486,790	-		PP_1300	0.75	+0.4
1,487,039	-				-0.2
1,487,062	-				-0.2
1,487,115	+				-1.2
1,487,116	-				+1.7
1,487,548	+		PP_1301	0.36	-0.4
1,487,548	+		PP_1301	0.36	-1.4
1,487,549	-				+2.7
1,487,640	+		PP_1301	0.44	+0.3
1,487,640	+		PP_1301	0.44	+0.0
1,487,640	+		PP_1301	0.44	-0.4
1,487,640	+		PP_1301	0.44	-0.9
1,487,640	+		PP_1301	0.44	-0.8
1,487,641	-		PP_1301	0.44	-1.2
1,487,876	+		PP_1301	0.63	-1.1
1,487,925	+		PP_1301	0.67	-1.0
1,487,925	+		PP_1301	0.67	-1.6
1,487,926	-		PP_1301	0.67	+0.2
1,488,066	+		PP_1301	0.79	-1.7
1,488,066	+		PP_1301	0.79	-0.5
1,488,066	+		PP_1301	0.79	-0.2
1,488,067	-		PP_1301	0.79	-0.3
1,488,111	+		PP_1301	0.82	-0.4
1,488,227	+				-1.8
1,488,227	+				+0.5
1,488,228	-				-0.1
1,488,235	+				-0.9
1,488,255	+				-1.8
1,488,255	+				-0.7
1,488,339	-				-0.1
1,488,372	-				+0.6
1,488,372	-				-0.0
1,488,384	+				-0.7
1,488,384	+				+0.9
1,488,512	+				-1.3
1,488,560	-		PP_1302	0.15	+2.3
1,488,645	-		PP_1302	0.27	+0.5
1,488,713	-		PP_1302	0.36	+1.1
1,488,718	+		PP_1302	0.36	-0.4
1,488,718	+		PP_1302	0.36	-0.6
1,488,718	+		PP_1302	0.36	+0.5
1,488,719	-		PP_1302	0.36	+1.1
1,488,719	-		PP_1302	0.36	-0.6
1,488,719	-		PP_1302	0.36	+0.1
1,488,719	-		PP_1302	0.36	+2.7
1,488,719	-		PP_1302	0.36	+1.1
1,488,720	+		PP_1302	0.36	+2.1
1,488,720	+		PP_1302	0.36	+0.5
1,488,720	+		PP_1302	0.36	-2.9
1,488,720	+		PP_1302	0.36	-1.0
1,488,720	+		PP_1302	0.36	-0.4
1,488,721	-		PP_1302	0.37	-0.3
1,488,721	-		PP_1302	0.37	+0.0
1,488,721	-		PP_1302	0.37	+0.2
1,488,721	-		PP_1302	0.37	+0.8
1,488,721	-		PP_1302	0.37	+0.6
1,488,721	-		PP_1302	0.37	+0.3
1,488,721	-		PP_1302	0.37	-1.2
1,488,820	-		PP_1302	0.50	+1.7
1,488,989	+		PP_1302	0.72	-0.0
1,488,989	+		PP_1302	0.72	-0.3
1,488,989	+		PP_1302	0.72	-0.1
1,488,989	+		PP_1302	0.72	-1.5
1,488,990	-		PP_1302	0.72	-0.5
1,488,990	-		PP_1302	0.72	+0.5
1,488,990	-		PP_1302	0.72	+0.3
1,488,991	+		PP_1302	0.72	+0.6
1,489,025	+		PP_1302	0.77	+0.5
1,489,055	+		PP_1302	0.81	+1.0
1,489,056	-		PP_1302	0.81	-0.4
1,489,142	+				-1.2
1,489,232	+				-0.3
1,489,232	+				+1.0
1,489,233	-				+1.0
1,489,233	-				+0.9
1,489,233	-				+1.2
1,489,288	-				-0.6
1,489,320	+				-0.2
1,489,320	+				-1.5
1,489,321	-				+1.7
1,489,321	-				-0.9