Strain Fitness in Pseudomonas putida KT2440 around PP_1025

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1023 and PP_1024 overlap by 10 nucleotidesPP_1024 and PP_1025 are separated by 252 nucleotidesPP_1025 and PP_1026 are separated by 432 nucleotides PP_1023: PP_1023 - 6-phosphogluconolactonase, at 1,167,057 to 1,167,785 _1023 PP_1024: PP_1024 - KHG/KDPG aldolase, at 1,167,776 to 1,168,483 _1024 PP_1025: PP_1025 - 2-isopropylmalate synthase, at 1,168,736 to 1,170,409 _1025 PP_1026: PP_1026 - Peptidase, M23/M37 family, at 1,170,842 to 1,171,669 _1026 Position (kb) 1168 1169 1170 1171Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1168.501 kb on - strandat 1168.547 kb on + strandat 1168.547 kb on + strandat 1168.548 kb on - strandat 1168.581 kb on - strandat 1168.592 kb on + strandat 1168.641 kb on - strandat 1168.643 kb on + strandat 1168.643 kb on + strandat 1168.644 kb on - strandat 1168.644 kb on - strandat 1168.644 kb on - strandat 1168.838 kb on + strandat 1168.839 kb on - strandat 1168.872 kb on + strandat 1168.872 kb on + strandat 1168.872 kb on + strandat 1168.873 kb on - strandat 1168.920 kb on + strand, within PP_1025at 1168.920 kb on + strand, within PP_1025at 1169.082 kb on + strand, within PP_1025at 1169.082 kb on + strand, within PP_1025at 1169.082 kb on + strand, within PP_1025at 1169.082 kb on + strand, within PP_1025at 1169.083 kb on - strand, within PP_1025at 1169.103 kb on + strand, within PP_1025at 1169.103 kb on + strand, within PP_1025at 1169.103 kb on + strand, within PP_1025at 1169.103 kb on + strand, within PP_1025at 1169.103 kb on + strand, within PP_1025at 1169.104 kb on - strand, within PP_1025at 1169.104 kb on - strand, within PP_1025at 1169.104 kb on - strand, within PP_1025at 1169.104 kb on - strand, within PP_1025at 1169.124 kb on + strand, within PP_1025at 1169.124 kb on + strand, within PP_1025at 1169.125 kb on - strand, within PP_1025at 1169.224 kb on - strand, within PP_1025at 1169.224 kb on - strand, within PP_1025at 1169.241 kb on + strand, within PP_1025at 1169.241 kb on + strand, within PP_1025at 1169.242 kb on - strand, within PP_1025at 1169.242 kb on - strand, within PP_1025at 1169.292 kb on + strand, within PP_1025at 1169.292 kb on + strand, within PP_1025at 1169.329 kb on - strand, within PP_1025at 1169.430 kb on + strand, within PP_1025at 1169.430 kb on + strand, within PP_1025at 1169.430 kb on + strand, within PP_1025at 1169.430 kb on + strand, within PP_1025at 1169.430 kb on + strand, within PP_1025at 1169.430 kb on + strand, within PP_1025at 1169.431 kb on - strand, within PP_1025at 1169.431 kb on - strand, within PP_1025at 1169.447 kb on + strand, within PP_1025at 1169.532 kb on + strand, within PP_1025at 1169.532 kb on + strand, within PP_1025at 1169.532 kb on + strand, within PP_1025at 1169.532 kb on + strand, within PP_1025at 1169.740 kb on - strand, within PP_1025at 1169.743 kb on + strand, within PP_1025at 1169.743 kb on + strand, within PP_1025at 1169.744 kb on - strand, within PP_1025at 1169.883 kb on + strand, within PP_1025at 1169.922 kb on + strand, within PP_1025at 1169.923 kb on - strand, within PP_1025at 1169.923 kb on - strand, within PP_1025at 1170.021 kb on + strand, within PP_1025at 1170.021 kb on + strand, within PP_1025at 1170.022 kb on - strand, within PP_1025at 1170.022 kb on - strand, within PP_1025at 1170.022 kb on - strand, within PP_1025at 1170.022 kb on - strand, within PP_1025at 1170.266 kb on + strandat 1170.310 kb on + strandat 1170.310 kb on + strandat 1170.311 kb on - strandat 1170.463 kb on - strandat 1170.463 kb on - strandat 1170.463 kb on - strandat 1170.495 kb on + strandat 1170.771 kb on + strandat 1170.772 kb on - strandat 1170.772 kb on - strandat 1170.772 kb on - strandat 1170.772 kb on - strandat 1170.799 kb on + strandat 1171.025 kb on - strand, within PP_1026at 1171.072 kb on - strand, within PP_1026at 1171.371 kb on + strand, within PP_1026at 1171.385 kb on + strand, within PP_1026

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Per-strain Table

Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
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1,168,501 - +0.5
1,168,547 + +0.8
1,168,547 + -0.2
1,168,548 - -2.3
1,168,581 - -0.2
1,168,592 + -0.5
1,168,641 - -0.2
1,168,643 + +1.4
1,168,643 + -0.3
1,168,644 - +0.8
1,168,644 - -0.2
1,168,644 - +1.0
1,168,838 + -1.8
1,168,839 - -0.8
1,168,872 + -1.2
1,168,872 + -1.9
1,168,872 + -2.3
1,168,873 - -1.8
1,168,920 + PP_1025 0.11 +0.5
1,168,920 + PP_1025 0.11 -1.3
1,169,082 + PP_1025 0.21 -1.3
1,169,082 + PP_1025 0.21 -0.1
1,169,082 + PP_1025 0.21 -1.6
1,169,082 + PP_1025 0.21 -1.1
1,169,083 - PP_1025 0.21 +1.2
1,169,103 + PP_1025 0.22 -0.6
1,169,103 + PP_1025 0.22 -2.6
1,169,103 + PP_1025 0.22 -2.5
1,169,103 + PP_1025 0.22 -1.0
1,169,103 + PP_1025 0.22 -0.8
1,169,104 - PP_1025 0.22 -2.4
1,169,104 - PP_1025 0.22 -1.8
1,169,104 - PP_1025 0.22 -1.8
1,169,104 - PP_1025 0.22 -0.4
1,169,124 + PP_1025 0.23 -2.6
1,169,124 + PP_1025 0.23 -1.7
1,169,125 - PP_1025 0.23 -3.5
1,169,224 - PP_1025 0.29 -2.8
1,169,224 - PP_1025 0.29 +2.7
1,169,241 + PP_1025 0.30 -3.3
1,169,241 + PP_1025 0.30 -1.2
1,169,242 - PP_1025 0.30 +1.7
1,169,242 - PP_1025 0.30 +0.4
1,169,292 + PP_1025 0.33 -2.7
1,169,292 + PP_1025 0.33 -1.0
1,169,329 - PP_1025 0.35 -1.5
1,169,430 + PP_1025 0.41 -2.9
1,169,430 + PP_1025 0.41 -2.7
1,169,430 + PP_1025 0.41 -3.4
1,169,430 + PP_1025 0.41 -2.3
1,169,430 + PP_1025 0.41 -2.7
1,169,430 + PP_1025 0.41 -2.7
1,169,431 - PP_1025 0.42 -2.7
1,169,431 - PP_1025 0.42 -2.4
1,169,447 + PP_1025 0.42 -2.4
1,169,532 + PP_1025 0.48 +0.4
1,169,532 + PP_1025 0.48 -2.7
1,169,532 + PP_1025 0.48 -1.8
1,169,532 + PP_1025 0.48 -1.6
1,169,740 - PP_1025 0.60 -2.7
1,169,743 + PP_1025 0.60 -3.0
1,169,743 + PP_1025 0.60 -2.4
1,169,744 - PP_1025 0.60 -2.5
1,169,883 + PP_1025 0.69 -1.8
1,169,922 + PP_1025 0.71 -1.6
1,169,923 - PP_1025 0.71 +0.4
1,169,923 - PP_1025 0.71 -0.1
1,170,021 + PP_1025 0.77 -3.5
1,170,021 + PP_1025 0.77 -2.7
1,170,022 - PP_1025 0.77 -1.6
1,170,022 - PP_1025 0.77 -4.0
1,170,022 - PP_1025 0.77 -2.6
1,170,022 - PP_1025 0.77 -3.1
1,170,266 + -3.5
1,170,310 + -1.3
1,170,310 + -1.6
1,170,311 - -1.8
1,170,463 - -0.2
1,170,463 - -0.0
1,170,463 - +0.7
1,170,495 + -3.0
1,170,771 + +0.0
1,170,772 - -0.2
1,170,772 - +0.3
1,170,772 - +2.3
1,170,772 - +2.7
1,170,799 + +0.3
1,171,025 - PP_1026 0.22 -0.6
1,171,072 - PP_1026 0.28 +1.1
1,171,371 + PP_1026 0.64 -2.3
1,171,385 + PP_1026 0.66 +0.1

Or see this region's nucleotide sequence