Strain Fitness in Pseudomonas putida KT2440 around PP_0978

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0977 and PP_0978 are separated by 83 nucleotidesPP_0978 and PP_0979 are separated by 5 nucleotidesPP_0979 and PP_0980 are separated by 50 nucleotides PP_0977: PP_0977 - valine--tRNA ligase, at 1,114,985 to 1,117,831 _0977 PP_0978: PP_0978 - conserved protein of unknown function, at 1,117,915 to 1,118,280 _0978 PP_0979: PP_0979 - DNA polymerase III subunit chi, at 1,118,286 to 1,118,714 _0979 PP_0980: PP_0980 - Probable cytosol aminopeptidase, at 1,118,765 to 1,120,258 _0980 Position (kb) 1117 1118 1119Strain fitness (log2 ratio) -2 -1 0 1at 1117.917 kb on - strandat 1117.980 kb on + strand, within PP_0978at 1117.981 kb on - strand, within PP_0978at 1118.167 kb on + strand, within PP_0978at 1118.298 kb on + strandat 1118.320 kb on + strandat 1118.321 kb on - strandat 1118.608 kb on + strand, within PP_0979at 1118.609 kb on - strand, within PP_0979at 1118.609 kb on - strand, within PP_0979at 1118.609 kb on - strand, within PP_0979at 1118.695 kb on + strandat 1118.695 kb on + strandat 1118.695 kb on + strandat 1118.696 kb on - strandat 1118.790 kb on + strandat 1118.790 kb on + strandat 1118.790 kb on + strandat 1118.791 kb on - strandat 1118.887 kb on - strandat 1118.887 kb on - strandat 1119.073 kb on + strand, within PP_0980at 1119.175 kb on - strand, within PP_0980

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Per-strain Table

Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
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1,117,917 - +1.3
1,117,980 + PP_0978 0.18 -1.0
1,117,981 - PP_0978 0.18 -1.8
1,118,167 + PP_0978 0.69 -1.5
1,118,298 + +0.9
1,118,320 + -0.4
1,118,321 - +1.2
1,118,608 + PP_0979 0.75 -0.4
1,118,609 - PP_0979 0.75 -0.1
1,118,609 - PP_0979 0.75 +1.1
1,118,609 - PP_0979 0.75 +0.4
1,118,695 + -1.3
1,118,695 + +0.1
1,118,695 + -1.5
1,118,696 - -0.9
1,118,790 + -0.9
1,118,790 + -1.0
1,118,790 + -0.2
1,118,791 - -0.2
1,118,887 - -1.4
1,118,887 - -1.6
1,119,073 + PP_0980 0.21 -1.1
1,119,175 - PP_0980 0.27 -1.7

Or see this region's nucleotide sequence