Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0767 and PP_0768 are separated by 159 nucleotides PP_0768 and PP_0769 overlap by 4 nucleotides PP_0769 and PP_0770 are separated by 193 nucleotides
PP_0767: PP_0767 - Transcriptional regulator, LuxR family, at 884,090 to 886,807
_0767
PP_0768: PP_0768 - Response regulator/TPR domain protein, at 886,967 to 888,574
_0768
PP_0769: PP_0769 - Sensor histidine kinase, at 888,571 to 889,263
_0769
PP_0770: PP_0770 - PemI-like protein, at 889,457 to 889,711
_0770
Position (kb)
886
887
888
889 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 886.100 kb on + strand, within PP_0767 at 886.100 kb on + strand, within PP_0767 at 886.101 kb on - strand, within PP_0767 at 886.200 kb on - strand, within PP_0767 at 886.200 kb on - strand, within PP_0767 at 886.200 kb on - strand, within PP_0767 at 886.200 kb on - strand, within PP_0767 at 886.200 kb on - strand, within PP_0767 at 886.297 kb on + strand, within PP_0767 at 886.333 kb on - strand, within PP_0767 at 886.367 kb on - strand, within PP_0767 at 886.367 kb on - strand, within PP_0767 at 886.367 kb on - strand, within PP_0767 at 886.367 kb on - strand, within PP_0767 at 886.600 kb on + strand at 886.809 kb on - strand at 886.820 kb on - strand at 886.820 kb on - strand at 886.820 kb on - strand at 886.820 kb on - strand at 886.820 kb on - strand at 886.834 kb on + strand at 886.835 kb on - strand at 886.835 kb on - strand at 886.856 kb on - strand at 886.856 kb on - strand at 886.856 kb on - strand at 886.858 kb on + strand at 886.858 kb on + strand at 886.859 kb on - strand at 886.878 kb on - strand at 886.878 kb on - strand at 886.878 kb on - strand at 886.878 kb on - strand at 886.906 kb on + strand at 886.907 kb on - strand at 886.912 kb on + strand at 886.977 kb on - strand at 887.024 kb on - strand at 887.024 kb on - strand at 887.120 kb on + strand at 887.120 kb on + strand at 887.121 kb on - strand at 887.294 kb on + strand, within PP_0768 at 887.294 kb on + strand, within PP_0768 at 887.412 kb on + strand, within PP_0768 at 887.565 kb on + strand, within PP_0768 at 887.566 kb on - strand, within PP_0768 at 887.566 kb on - strand, within PP_0768 at 887.567 kb on + strand, within PP_0768 at 887.567 kb on + strand, within PP_0768 at 887.568 kb on - strand, within PP_0768 at 887.669 kb on + strand, within PP_0768 at 887.867 kb on + strand, within PP_0768 at 888.221 kb on + strand, within PP_0768 at 888.333 kb on - strand, within PP_0768 at 888.530 kb on + strand at 888.779 kb on - strand, within PP_0769 at 888.779 kb on - strand, within PP_0769 at 888.806 kb on + strand, within PP_0769 at 888.806 kb on + strand, within PP_0769 at 888.817 kb on + strand, within PP_0769 at 888.869 kb on + strand, within PP_0769 at 888.869 kb on + strand, within PP_0769 at 888.870 kb on - strand, within PP_0769 at 888.870 kb on - strand, within PP_0769 at 889.103 kb on - strand, within PP_0769 at 889.103 kb on - strand, within PP_0769 at 889.103 kb on - strand, within PP_0769 at 889.151 kb on - strand, within PP_0769 at 889.211 kb on + strand at 889.211 kb on + strand at 889.211 kb on + strand at 889.257 kb on - strand at 889.278 kb on + strand at 889.278 kb on + strand at 889.324 kb on + strand at 889.324 kb on + strand at 889.382 kb on + strand at 889.383 kb on - strand at 889.383 kb on - strand at 889.383 kb on - strand at 889.383 kb on - strand at 889.387 kb on - strand at 889.430 kb on - strand at 889.430 kb on - strand at 889.432 kb on + strand at 889.432 kb on + strand at 889.432 kb on + strand at 889.432 kb on + strand at 889.432 kb on + strand at 889.432 kb on + strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.440 kb on + strand at 889.441 kb on - strand at 889.452 kb on + strand at 889.453 kb on - strand at 889.453 kb on - strand at 889.453 kb on - strand at 889.515 kb on + strand, within PP_0770
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 886,100 + PP_0767 0.74 +3.7 886,100 + PP_0767 0.74 +0.9 886,101 - PP_0767 0.74 -2.5 886,200 - PP_0767 0.78 -0.0 886,200 - PP_0767 0.78 -0.8 886,200 - PP_0767 0.78 +0.0 886,200 - PP_0767 0.78 +0.7 886,200 - PP_0767 0.78 -0.2 886,297 + PP_0767 0.81 +0.5 886,333 - PP_0767 0.83 +0.8 886,367 - PP_0767 0.84 -0.6 886,367 - PP_0767 0.84 -2.5 886,367 - PP_0767 0.84 -2.1 886,367 - PP_0767 0.84 -0.8 886,600 + +0.4 886,809 - +0.9 886,820 - -0.4 886,820 - +1.3 886,820 - +0.7 886,820 - +0.8 886,820 - -0.7 886,834 + +0.4 886,835 - +1.7 886,835 - -0.2 886,856 - -0.1 886,856 - +0.5 886,856 - -0.8 886,858 + +0.8 886,858 + +1.7 886,859 - -0.1 886,878 - -1.6 886,878 - -0.1 886,878 - -0.5 886,878 - +0.5 886,906 + -3.1 886,907 - -0.2 886,912 + -0.8 886,977 - +0.4 887,024 - -0.6 887,024 - -0.4 887,120 + +0.4 887,120 + +0.8 887,121 - -0.8 887,294 + PP_0768 0.20 +0.7 887,294 + PP_0768 0.20 -0.1 887,412 + PP_0768 0.28 -0.6 887,565 + PP_0768 0.37 +1.1 887,566 - PP_0768 0.37 +2.1 887,566 - PP_0768 0.37 +0.0 887,567 + PP_0768 0.37 -1.6 887,567 + PP_0768 0.37 -0.1 887,568 - PP_0768 0.37 -1.4 887,669 + PP_0768 0.44 -0.5 887,867 + PP_0768 0.56 -1.4 888,221 + PP_0768 0.78 -1.6 888,333 - PP_0768 0.85 -1.3 888,530 + +0.2 888,779 - PP_0769 0.30 +0.8 888,779 - PP_0769 0.30 +0.6 888,806 + PP_0769 0.34 +0.6 888,806 + PP_0769 0.34 -1.6 888,817 + PP_0769 0.35 -0.2 888,869 + PP_0769 0.43 -0.6 888,869 + PP_0769 0.43 -0.6 888,870 - PP_0769 0.43 +1.1 888,870 - PP_0769 0.43 -0.2 889,103 - PP_0769 0.77 -1.6 889,103 - PP_0769 0.77 -2.2 889,103 - PP_0769 0.77 -0.6 889,151 - PP_0769 0.84 -0.1 889,211 + +0.0 889,211 + +0.1 889,211 + +0.5 889,257 - -0.6 889,278 + -0.3 889,278 + +2.1 889,324 + -0.7 889,324 + -1.0 889,382 + +0.3 889,383 - +0.1 889,383 - +0.6 889,383 - -0.6 889,383 - -0.1 889,387 - -0.2 889,430 - +0.8 889,430 - -0.0 889,432 + +1.3 889,432 + +0.7 889,432 + -1.3 889,432 + -0.2 889,432 + -0.6 889,432 + +0.1 889,433 - -0.4 889,433 - +0.1 889,433 - -0.0 889,433 - -0.0 889,433 - -0.3 889,433 - +0.6 889,433 - +0.8 889,433 - +0.2 889,433 - +0.5 889,436 + +1.3 889,436 + +0.4 889,436 + +0.9 889,436 + -0.8 889,436 + -0.6 889,436 + -1.4 889,436 + -0.0 889,437 - +0.4 889,437 - -0.1 889,437 - -0.3 889,437 - +0.1 889,437 - -0.6 889,437 - -0.7 889,437 - +0.9 889,437 - +0.1 889,437 - +0.7 889,437 - -1.3 889,440 + +0.3 889,441 - -0.6 889,452 + +0.4 889,453 - -0.6 889,453 - +0.5 889,453 - +0.3 889,515 + PP_0770 0.23 +1.2
Or see this region's nucleotide sequence