Strain Fitness in Pseudomonas putida KT2440 around PP_0746

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
864,248	+		PP_0745	0.26	+0.2
864,248	+		PP_0745	0.26	-0.3
864,249	-		PP_0745	0.26	+1.1
864,289	+		PP_0745	0.29	+0.7
864,290	-		PP_0745	0.29	+0.7
864,290	-		PP_0745	0.29	+1.7
864,290	-		PP_0745	0.29	-0.4
864,328	+		PP_0745	0.32	+1.3
864,328	+				+0.3
864,328	+		PP_0745	0.32	+0.9
864,329	-		PP_0745	0.32	+0.1
864,368	+		PP_0745	0.35	-0.0
864,422	-		PP_0745	0.40	-0.8
864,422	-		PP_0745	0.40	-0.0
864,728	-		PP_0745	0.64	-0.6
864,895	+		PP_0745	0.77	-1.1
864,895	+		PP_0745	0.77	+0.0
864,895	+		PP_0745	0.77	+0.9
864,895	+		PP_0745	0.77	+0.6
864,896	-		PP_0745	0.77	-0.3
864,896	-		PP_0745	0.77	-1.7
864,896	-		PP_0745	0.77	+0.2
865,005	+		PP_0745	0.86	+1.4
865,006	-		PP_0745	0.86	+0.7
865,032	+		PP_0745	0.88	+0.4
865,033	-		PP_0745	0.88	+1.1
865,033	-		PP_0745	0.88	+0.2
865,252	-		PP_0746	0.10	+0.9
865,252	-		PP_0746	0.10	-0.9
865,488	+		PP_0746	0.47	-1.0
865,488	+		PP_0746	0.47	+1.1
865,488	+		PP_0746	0.47	+0.3
865,488	+		PP_0746	0.47	+0.2
865,489	-		PP_0746	0.47	+2.1
865,489	-		PP_0746	0.47	-0.3
865,489	-		PP_0746	0.47	-1.1
865,489	-		PP_0746	0.47	-0.2
865,605	-		PP_0746	0.65	-0.7
865,605	-		PP_0746	0.65	+0.1
865,607	+		PP_0746	0.66	+0.2
865,607	+		PP_0746	0.66	-0.5
865,608	-		PP_0746	0.66	+0.2
865,672	-		PP_0746	0.76	+0.7
865,672	-		PP_0746	0.76	+0.0
865,704	+		PP_0746	0.81	-2.0
865,705	-		PP_0746	0.81	+1.1
865,705	-		PP_0746	0.81	+0.8
865,705	-		PP_0746	0.81	-0.1
865,864	-				+0.2
865,868	+				-0.4
865,869	-				-0.2
865,869	-				+0.3
865,869	-				+0.6
865,871	+				+1.8
865,873	+				-1.3
865,874	-				+0.6
865,874	-				+0.4
865,874	-				-0.0
865,910	+				+0.7
865,910	+				+2.1
865,911	-				-0.1
865,917	+				+0.7
865,918	-				-1.4
865,918	-				+1.5
865,948	-				+0.6
865,948	-				+0.0
866,008	+				-0.3
866,008	+				+0.5
866,008	+				+0.2
866,009	-				+0.6
866,042	+				+0.4
866,042	+				+0.5
866,201	+		PP_0747	0.38	-0.7
866,308	+		PP_0747	0.57	-0.1
866,378	+		PP_0747	0.70	-0.5
866,378	+		PP_0747	0.70	+2.2
866,441	+		PP_0747	0.81	-0.5
866,441	+		PP_0747	0.81	+1.1
866,441	+		PP_0747	0.81	-1.8
866,441	+		PP_0747	0.81	-0.7
866,441	+		PP_0747	0.81	-2.0
866,441	+		PP_0747	0.81	+2.7
866,441	+		PP_0747	0.81	+0.8
866,441	+		PP_0747	0.81	-0.5
866,441	+		PP_0747	0.81	+1.7
866,442	-		PP_0747	0.81	+0.4
866,442	-		PP_0747	0.81	-0.1
866,442	-		PP_0747	0.81	-0.8
866,442	-		PP_0747	0.81	+0.5
866,442	-		PP_0747	0.81	+0.7
866,442	-		PP_0747	0.81	-0.6
866,442	-		PP_0747	0.81	-0.9
866,442	-		PP_0747	0.81	+0.3
866,442	-		PP_0747	0.81	-0.1
866,468	+		PP_0747	0.86	+1.2
866,468	+		PP_0747	0.86	-0.0
866,469	-		PP_0747	0.86	-1.4
866,469	-		PP_0747	0.86	-1.1
866,469	-		PP_0747	0.86	+0.2
866,469	-		PP_0747	0.86	-1.1
866,469	-		PP_0747	0.86	+1.0
866,470	+		PP_0747	0.86	-2.0
866,470	+		PP_0747	0.86	-0.6
866,471	-		PP_0747	0.87	+0.7
866,471	-		PP_0747	0.87	-0.0
866,481	-		PP_0747	0.88	+0.5
866,492	+				-0.7
866,492	+				+0.4
866,492	+				-0.4
866,492	+				+0.5
866,493	-				+1.7
866,502	-				+0.0
866,647	-		PP_0748	0.21	-0.3
866,695	+		PP_0748	0.31	+1.1
866,696	-		PP_0748	0.31	+0.3
866,696	-		PP_0748	0.31	+1.3