Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0682 and PP_0683 are separated by 76 nucleotides PP_0683 and PP_0684 are separated by 85 nucleotides PP_0684 and PP_0685 are separated by 26 nucleotides PP_0685 and PP_0686 are separated by 84 nucleotides PP_0686 and PP_0687 are separated by 160 nucleotides
PP_0682: PP_0682 - conserved inner membrane protein of unknown function, UPF0114 family, at 796,988 to 797,476
_0682
PP_0683: PP_0683 - conserved protein of unknown function, at 797,553 to 797,942
_0683
PP_0684: PP_0684 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 798,028 to 798,645
_0684
PP_0685: PP_0685 - conserved protein of unknown function, at 798,672 to 798,917
_0685
PP_0686: PP_0686 - conserved protein of unknown function, at 799,002 to 799,343
_0686
PP_0687: PP_0687 - octaprenyl diphosphate synthase, at 799,504 to 800,472
_0687
Position (kb)
798
799 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 797.045 kb on + strand, within PP_0682 at 797.045 kb on + strand, within PP_0682 at 797.046 kb on - strand, within PP_0682 at 797.390 kb on + strand, within PP_0682 at 797.402 kb on + strand, within PP_0682 at 797.402 kb on + strand, within PP_0682 at 797.402 kb on + strand, within PP_0682 at 797.403 kb on - strand, within PP_0682 at 797.403 kb on - strand, within PP_0682 at 797.533 kb on + strand at 797.591 kb on - strand at 797.718 kb on + strand, within PP_0683 at 797.719 kb on - strand, within PP_0683 at 797.719 kb on - strand, within PP_0683 at 797.719 kb on - strand, within PP_0683 at 797.940 kb on + strand at 797.940 kb on + strand at 797.967 kb on - strand at 798.070 kb on + strand at 798.070 kb on + strand at 798.070 kb on + strand at 798.399 kb on + strand, within PP_0684 at 798.400 kb on - strand, within PP_0684 at 798.407 kb on - strand at 798.448 kb on + strand at 798.448 kb on + strand, within PP_0684 at 798.547 kb on + strand, within PP_0684 at 798.547 kb on + strand, within PP_0684 at 798.547 kb on + strand, within PP_0684 at 798.547 kb on + strand, within PP_0684 at 798.547 kb on + strand, within PP_0684 at 798.548 kb on - strand, within PP_0684 at 798.548 kb on - strand, within PP_0684 at 798.548 kb on - strand, within PP_0684 at 798.548 kb on - strand, within PP_0684 at 798.548 kb on - strand, within PP_0684 at 798.548 kb on - strand, within PP_0684 at 798.569 kb on - strand, within PP_0684 at 798.643 kb on + strand at 798.644 kb on - strand at 798.644 kb on - strand at 798.713 kb on - strand, within PP_0685 at 798.713 kb on - strand, within PP_0685 at 798.874 kb on + strand, within PP_0685 at 798.910 kb on + strand at 798.910 kb on + strand at 798.911 kb on - strand at 798.911 kb on - strand at 798.921 kb on + strand at 798.921 kb on + strand at 798.922 kb on - strand at 798.922 kb on - strand at 798.922 kb on - strand at 799.297 kb on + strand, within PP_0686 at 799.297 kb on + strand, within PP_0686 at 799.297 kb on + strand, within PP_0686 at 799.297 kb on + strand, within PP_0686 at 799.297 kb on + strand, within PP_0686 at 799.297 kb on + strand, within PP_0686 at 799.297 kb on + strand, within PP_0686 at 799.297 kb on + strand, within PP_0686 at 799.297 kb on + strand, within PP_0686 at 799.297 kb on + strand, within PP_0686 at 799.298 kb on - strand, within PP_0686 at 799.298 kb on - strand, within PP_0686 at 799.298 kb on - strand, within PP_0686 at 799.298 kb on - strand, within PP_0686 at 799.298 kb on - strand, within PP_0686 at 799.298 kb on - strand, within PP_0686 at 799.298 kb on - strand, within PP_0686 at 799.298 kb on - strand, within PP_0686 at 799.298 kb on - strand, within PP_0686 at 799.298 kb on - strand, within PP_0686 at 799.303 kb on + strand, within PP_0686 at 799.303 kb on + strand, within PP_0686 at 799.303 kb on + strand, within PP_0686 at 799.304 kb on - strand, within PP_0686 at 799.304 kb on - strand, within PP_0686 at 799.304 kb on - strand, within PP_0686 at 799.304 kb on - strand, within PP_0686 at 799.374 kb on + strand at 799.374 kb on + strand at 799.389 kb on + strand at 799.390 kb on - strand at 799.390 kb on - strand at 799.396 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 797,045 + PP_0682 0.12 +1.7 797,045 + PP_0682 0.12 +0.1 797,046 - PP_0682 0.12 +1.5 797,390 + PP_0682 0.82 +0.8 797,402 + PP_0682 0.85 +0.7 797,402 + PP_0682 0.85 -0.5 797,402 + PP_0682 0.85 +0.2 797,403 - PP_0682 0.85 +0.8 797,403 - PP_0682 0.85 -0.2 797,533 + -0.2 797,591 - -1.2 797,718 + PP_0683 0.42 -0.1 797,719 - PP_0683 0.43 +0.8 797,719 - PP_0683 0.43 -0.2 797,719 - PP_0683 0.43 +0.1 797,940 + -0.6 797,940 + +0.5 797,967 - +0.8 798,070 + +1.1 798,070 + +0.4 798,070 + -0.2 798,399 + PP_0684 0.60 -0.4 798,400 - PP_0684 0.60 -2.6 798,407 - +0.1 798,448 + +0.7 798,448 + PP_0684 0.68 -1.1 798,547 + PP_0684 0.84 -0.2 798,547 + PP_0684 0.84 -1.2 798,547 + PP_0684 0.84 -1.9 798,547 + PP_0684 0.84 -0.1 798,547 + PP_0684 0.84 -1.8 798,548 - PP_0684 0.84 -0.5 798,548 - PP_0684 0.84 -0.6 798,548 - PP_0684 0.84 -1.8 798,548 - PP_0684 0.84 -0.1 798,548 - PP_0684 0.84 +0.7 798,548 - PP_0684 0.84 -0.7 798,569 - PP_0684 0.88 -1.9 798,643 + -1.4 798,644 - -0.2 798,644 - +0.2 798,713 - PP_0685 0.17 +0.2 798,713 - PP_0685 0.17 +0.7 798,874 + PP_0685 0.82 +0.6 798,910 + -0.1 798,910 + -0.3 798,911 - -0.1 798,911 - +0.3 798,921 + +0.6 798,921 + -1.0 798,922 - -0.8 798,922 - +1.4 798,922 - -0.0 799,297 + PP_0686 0.86 +2.7 799,297 + PP_0686 0.86 -0.6 799,297 + PP_0686 0.86 -0.7 799,297 + PP_0686 0.86 +0.0 799,297 + PP_0686 0.86 +0.7 799,297 + PP_0686 0.86 +0.7 799,297 + PP_0686 0.86 +1.1 799,297 + PP_0686 0.86 -0.1 799,297 + PP_0686 0.86 +0.5 799,297 + PP_0686 0.86 -0.3 799,298 - PP_0686 0.87 -0.7 799,298 - PP_0686 0.87 -0.7 799,298 - PP_0686 0.87 +1.7 799,298 - PP_0686 0.87 +0.0 799,298 - PP_0686 0.87 -0.2 799,298 - PP_0686 0.87 -0.2 799,298 - PP_0686 0.87 -0.4 799,298 - PP_0686 0.87 -1.0 799,298 - PP_0686 0.87 +0.2 799,298 - PP_0686 0.87 +2.3 799,303 + PP_0686 0.88 +0.2 799,303 + PP_0686 0.88 -0.3 799,303 + PP_0686 0.88 +1.1 799,304 - PP_0686 0.88 -0.5 799,304 - PP_0686 0.88 -2.2 799,304 - PP_0686 0.88 +0.1 799,304 - PP_0686 0.88 +0.9 799,374 + -2.2 799,374 + +2.7 799,389 + +2.7 799,390 - +1.9 799,390 - +0.0 799,396 + -0.3
Or see this region's nucleotide sequence