Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0376 and PP_0377 are separated by 1 nucleotides PP_0377 and PP_0378 overlap by 4 nucleotides PP_0378 and PP_0379 are separated by 163 nucleotides
PP_0376: PP_0376 - Coenzyme PQQ synthesis protein E, at 456,620 to 457,750
_0376
PP_0377: PP_0377 - coenzyme PQQ synthesis protein D, at 457,752 to 458,027
_0377
PP_0378: PP_0378 - Pyrroloquinoline-quinone synthase, at 458,024 to 458,779
_0378
PP_0379: PP_0379 - Coenzyme PQQ synthesis protein B, at 458,943 to 459,854
_0379
Position (kb) 457
458
459 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 456.859 kb on - strand at 456.898 kb on + strand, within PP_0376 at 456.898 kb on + strand, within PP_0376 at 456.898 kb on + strand, within PP_0376 at 456.898 kb on + strand, within PP_0376 at 456.898 kb on + strand, within PP_0376 at 456.898 kb on + strand, within PP_0376 at 456.899 kb on - strand, within PP_0376 at 456.899 kb on - strand, within PP_0376 at 456.899 kb on - strand, within PP_0376 at 456.899 kb on - strand, within PP_0376 at 456.920 kb on + strand, within PP_0376 at 456.920 kb on + strand, within PP_0376 at 456.921 kb on - strand, within PP_0376 at 456.974 kb on - strand, within PP_0376 at 457.041 kb on + strand, within PP_0376 at 457.041 kb on + strand, within PP_0376 at 457.042 kb on - strand, within PP_0376 at 457.045 kb on - strand, within PP_0376 at 457.045 kb on - strand, within PP_0376 at 457.117 kb on + strand, within PP_0376 at 457.118 kb on - strand, within PP_0376 at 457.118 kb on - strand, within PP_0376 at 457.161 kb on - strand, within PP_0376 at 457.185 kb on + strand, within PP_0376 at 457.186 kb on - strand, within PP_0376 at 457.186 kb on - strand, within PP_0376 at 457.492 kb on - strand, within PP_0376 at 457.492 kb on - strand, within PP_0376 at 457.494 kb on + strand, within PP_0376 at 457.495 kb on - strand, within PP_0376 at 457.671 kb on + strand at 457.696 kb on - strand at 457.742 kb on - strand at 457.742 kb on - strand at 457.939 kb on + strand, within PP_0377 at 457.973 kb on - strand, within PP_0377 at 458.005 kb on - strand at 458.012 kb on + strand at 458.027 kb on + strand at 458.027 kb on + strand at 458.028 kb on - strand at 458.119 kb on + strand, within PP_0378 at 458.120 kb on - strand, within PP_0378 at 458.161 kb on + strand, within PP_0378 at 458.161 kb on + strand, within PP_0378 at 458.161 kb on + strand, within PP_0378 at 458.162 kb on - strand, within PP_0378 at 458.187 kb on + strand, within PP_0378 at 458.188 kb on - strand, within PP_0378 at 458.188 kb on - strand, within PP_0378 at 458.256 kb on + strand, within PP_0378 at 458.256 kb on + strand, within PP_0378 at 458.257 kb on - strand, within PP_0378 at 458.430 kb on - strand, within PP_0378 at 458.430 kb on - strand, within PP_0378 at 458.430 kb on - strand, within PP_0378 at 458.622 kb on + strand, within PP_0378 at 458.622 kb on + strand, within PP_0378 at 458.623 kb on - strand, within PP_0378 at 458.625 kb on + strand, within PP_0378 at 458.625 kb on + strand, within PP_0378 at 458.626 kb on - strand, within PP_0378 at 458.694 kb on + strand, within PP_0378 at 458.694 kb on + strand, within PP_0378 at 458.695 kb on - strand, within PP_0378 at 458.712 kb on + strand at 458.712 kb on + strand at 458.712 kb on + strand at 458.713 kb on - strand at 458.713 kb on - strand at 458.713 kb on - strand at 458.715 kb on + strand at 458.716 kb on - strand at 458.795 kb on - strand at 458.804 kb on - strand at 458.804 kb on - strand at 458.940 kb on + strand at 458.944 kb on + strand at 458.944 kb on + strand at 458.945 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 456,859 - +2.7 456,898 + PP_0376 0.25 -0.9 456,898 + PP_0376 0.25 +0.1 456,898 + PP_0376 0.25 -0.6 456,898 + PP_0376 0.25 +0.0 456,898 + PP_0376 0.25 -0.8 456,898 + PP_0376 0.25 -0.6 456,899 - PP_0376 0.25 -1.8 456,899 - PP_0376 0.25 -0.2 456,899 - PP_0376 0.25 -0.3 456,899 - PP_0376 0.25 -0.5 456,920 + PP_0376 0.27 +0.0 456,920 + PP_0376 0.27 -0.8 456,921 - PP_0376 0.27 +0.0 456,974 - PP_0376 0.31 -2.7 457,041 + PP_0376 0.37 -1.1 457,041 + PP_0376 0.37 +0.1 457,042 - PP_0376 0.37 +0.1 457,045 - PP_0376 0.38 +0.7 457,045 - PP_0376 0.38 -0.2 457,117 + PP_0376 0.44 +0.4 457,118 - PP_0376 0.44 -0.0 457,118 - PP_0376 0.44 -1.8 457,161 - PP_0376 0.48 -2.9 457,185 + PP_0376 0.50 +0.1 457,186 - PP_0376 0.50 +2.7 457,186 - PP_0376 0.50 +1.7 457,492 - PP_0376 0.77 -0.5 457,492 - PP_0376 0.77 -0.3 457,494 + PP_0376 0.77 -0.8 457,495 - PP_0376 0.77 +0.3 457,671 + +2.7 457,696 - -1.5 457,742 - -0.9 457,742 - -0.4 457,939 + PP_0377 0.68 -0.6 457,973 - PP_0377 0.80 -2.5 458,005 - +2.7 458,012 + -1.0 458,027 + +0.3 458,027 + -1.2 458,028 - +0.8 458,119 + PP_0378 0.13 -0.3 458,120 - PP_0378 0.13 +0.5 458,161 + PP_0378 0.18 -1.4 458,161 + PP_0378 0.18 +0.4 458,161 + PP_0378 0.18 -0.1 458,162 - PP_0378 0.18 -0.1 458,187 + PP_0378 0.22 -0.3 458,188 - PP_0378 0.22 +0.1 458,188 - PP_0378 0.22 +0.2 458,256 + PP_0378 0.31 +0.8 458,256 + PP_0378 0.31 -0.8 458,257 - PP_0378 0.31 +0.7 458,430 - PP_0378 0.54 +2.0 458,430 - PP_0378 0.54 -0.8 458,430 - PP_0378 0.54 -0.3 458,622 + PP_0378 0.79 -0.1 458,622 + PP_0378 0.79 -1.5 458,623 - PP_0378 0.79 -1.2 458,625 + PP_0378 0.79 -0.2 458,625 + PP_0378 0.79 +1.4 458,626 - PP_0378 0.80 -1.3 458,694 + PP_0378 0.89 -0.9 458,694 + PP_0378 0.89 -2.0 458,695 - PP_0378 0.89 -1.5 458,712 + +0.1 458,712 + -1.8 458,712 + +0.0 458,713 - -1.4 458,713 - +0.8 458,713 - +1.7 458,715 + -0.7 458,716 - +0.5 458,795 - -0.6 458,804 - +0.5 458,804 - -1.1 458,940 + +0.6 458,944 + -0.2 458,944 + -0.3 458,945 - -1.6
Or see this region's nucleotide sequence