Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0289 and PP_0290 are separated by 0 nucleotides PP_0290 and PP_0291 are separated by 0 nucleotides PP_0291 and PP_0292 are separated by 27 nucleotides PP_0292 and PP_0293 are separated by 10 nucleotides
PP_0289: PP_0289 - Imidazoleglycerol-phosphate dehydratase, at 349,955 to 350,587
_0289
PP_0290: PP_0290 - Imidazole glycerol phosphate synthase subunit HisH, at 350,588 to 351,226
_0290
PP_0291: PP_0291 - conserved protein of unknown function, at 351,227 to 351,487
_0291
PP_0292: PP_0292 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) , at 351,515 to 352,252
_0292
PP_0293: PP_0293 - Imidazole glycerol phosphate synthase subunit HisF, at 352,263 to 353,033
_0293
Position (kb)
351
352 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 350.260 kb on + strand, within PP_0289 at 350.260 kb on + strand, within PP_0289 at 350.271 kb on - strand, within PP_0289 at 350.282 kb on + strand, within PP_0289 at 350.282 kb on + strand, within PP_0289 at 350.282 kb on + strand, within PP_0289 at 350.283 kb on - strand, within PP_0289 at 350.355 kb on + strand, within PP_0289 at 350.356 kb on - strand, within PP_0289 at 350.356 kb on - strand, within PP_0289 at 350.356 kb on - strand, within PP_0289 at 350.360 kb on + strand, within PP_0289 at 350.360 kb on + strand, within PP_0289 at 350.361 kb on - strand, within PP_0289 at 350.361 kb on - strand, within PP_0289 at 350.361 kb on - strand, within PP_0289 at 350.361 kb on - strand, within PP_0289 at 350.449 kb on + strand, within PP_0289 at 350.449 kb on + strand, within PP_0289 at 350.548 kb on + strand at 350.548 kb on + strand at 350.548 kb on + strand at 350.549 kb on - strand at 350.585 kb on + strand at 350.612 kb on + strand at 350.658 kb on + strand, within PP_0290 at 350.658 kb on + strand, within PP_0290 at 350.659 kb on - strand, within PP_0290 at 350.659 kb on - strand, within PP_0290 at 350.668 kb on + strand, within PP_0290 at 350.680 kb on + strand, within PP_0290 at 351.035 kb on + strand, within PP_0290 at 351.050 kb on + strand, within PP_0290 at 351.050 kb on + strand, within PP_0290 at 351.051 kb on - strand, within PP_0290 at 351.051 kb on - strand, within PP_0290 at 351.051 kb on - strand, within PP_0290 at 351.053 kb on + strand, within PP_0290 at 351.053 kb on + strand, within PP_0290 at 351.053 kb on + strand, within PP_0290 at 351.054 kb on - strand, within PP_0290 at 351.054 kb on - strand, within PP_0290 at 351.098 kb on + strand, within PP_0290 at 351.099 kb on - strand, within PP_0290 at 351.224 kb on + strand at 351.224 kb on + strand at 351.225 kb on - strand at 351.396 kb on - strand, within PP_0291 at 351.396 kb on - strand, within PP_0291 at 351.398 kb on + strand, within PP_0291 at 351.398 kb on + strand, within PP_0291 at 351.399 kb on - strand, within PP_0291 at 351.399 kb on - strand, within PP_0291 at 351.399 kb on - strand, within PP_0291 at 351.399 kb on - strand, within PP_0291 at 351.499 kb on + strand at 351.499 kb on + strand at 351.523 kb on + strand at 351.524 kb on - strand at 351.590 kb on - strand, within PP_0292 at 351.791 kb on + strand, within PP_0292 at 351.792 kb on - strand, within PP_0292 at 351.792 kb on - strand, within PP_0292 at 351.792 kb on - strand, within PP_0292 at 351.812 kb on + strand at 351.813 kb on - strand, within PP_0292 at 351.813 kb on - strand, within PP_0292 at 352.193 kb on + strand at 352.193 kb on + strand at 352.194 kb on - strand at 352.194 kb on - strand at 352.194 kb on - strand at 352.194 kb on - strand at 352.241 kb on + strand at 352.242 kb on - strand at 352.242 kb on - strand at 352.242 kb on - strand at 352.242 kb on - strand at 352.242 kb on - strand at 352.242 kb on - strand at 352.457 kb on + strand, within PP_0293 at 352.457 kb on + strand, within PP_0293 at 352.457 kb on + strand, within PP_0293
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 350,260 + PP_0289 0.48 -0.9 350,260 + PP_0289 0.48 -1.4 350,271 - PP_0289 0.50 +0.6 350,282 + PP_0289 0.52 -2.9 350,282 + PP_0289 0.52 -1.3 350,282 + PP_0289 0.52 -2.1 350,283 - PP_0289 0.52 -1.5 350,355 + PP_0289 0.63 -2.3 350,356 - PP_0289 0.63 -0.3 350,356 - PP_0289 0.63 -0.1 350,356 - PP_0289 0.63 +1.6 350,360 + PP_0289 0.64 -2.4 350,360 + PP_0289 0.64 -0.9 350,361 - PP_0289 0.64 -2.4 350,361 - PP_0289 0.64 -2.5 350,361 - PP_0289 0.64 +2.6 350,361 - PP_0289 0.64 -1.9 350,449 + PP_0289 0.78 -1.7 350,449 + PP_0289 0.78 -0.9 350,548 + +0.6 350,548 + +2.6 350,548 + +0.6 350,549 - -0.5 350,585 + -1.2 350,612 + -1.5 350,658 + PP_0290 0.11 -1.7 350,658 + PP_0290 0.11 -3.4 350,659 - PP_0290 0.11 -1.9 350,659 - PP_0290 0.11 -1.4 350,668 + PP_0290 0.13 -2.4 350,680 + PP_0290 0.14 -1.8 351,035 + PP_0290 0.70 -2.4 351,050 + PP_0290 0.72 -2.4 351,050 + PP_0290 0.72 -3.3 351,051 - PP_0290 0.72 -2.2 351,051 - PP_0290 0.72 -0.8 351,051 - PP_0290 0.72 -1.2 351,053 + PP_0290 0.73 -1.9 351,053 + PP_0290 0.73 -0.7 351,053 + PP_0290 0.73 -2.2 351,054 - PP_0290 0.73 -0.8 351,054 - PP_0290 0.73 -3.2 351,098 + PP_0290 0.80 -1.5 351,099 - PP_0290 0.80 -2.4 351,224 + -0.4 351,224 + -0.4 351,225 - -1.9 351,396 - PP_0291 0.65 -2.1 351,396 - PP_0291 0.65 -0.5 351,398 + PP_0291 0.66 -2.1 351,398 + PP_0291 0.66 +1.2 351,399 - PP_0291 0.66 -1.9 351,399 - PP_0291 0.66 -0.3 351,399 - PP_0291 0.66 +1.1 351,399 - PP_0291 0.66 -0.4 351,499 + -1.3 351,499 + -2.6 351,523 + +1.6 351,524 - -0.7 351,590 - PP_0292 0.10 -1.8 351,791 + PP_0292 0.37 -0.7 351,792 - PP_0292 0.38 -1.8 351,792 - PP_0292 0.38 +0.1 351,792 - PP_0292 0.38 -1.4 351,812 + +0.3 351,813 - PP_0292 0.40 -1.3 351,813 - PP_0292 0.40 -2.6 352,193 + -1.9 352,193 + -2.7 352,194 - -0.8 352,194 - -0.4 352,194 - -1.7 352,194 - -1.3 352,241 + -0.2 352,242 - -1.9 352,242 - -1.6 352,242 - +1.1 352,242 - -2.2 352,242 - -1.8 352,242 - -1.7 352,457 + PP_0293 0.25 -2.4 352,457 + PP_0293 0.25 -0.9 352,457 + PP_0293 0.25 -1.4
Or see this region's nucleotide sequence