Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0235 and PP_0236 are separated by 219 nucleotides PP_0236 and PP_0237 are separated by 85 nucleotides
PP_0235: PP_0235 - Peroxidase, at 290,082 to 290,720
_0235
PP_0236: PP_0236 - NAD(P)H-dependent FMN reductase subunit, at 290,940 to 291,533
_0236
PP_0237: PP_0237 - aliphatic sulfonate ABC transporter - periplasmic binding protein / transport of isethionate, at 291,619 to 292,584
_0237
Position (kb) 290
291
292 Strain fitness (log2 ratio) -2
-1
0
1
2 at 289.974 kb on - strand at 290.048 kb on + strand at 290.048 kb on + strand at 290.049 kb on - strand at 290.049 kb on - strand at 290.348 kb on + strand, within PP_0235 at 290.349 kb on - strand, within PP_0235 at 290.404 kb on - strand, within PP_0235 at 290.404 kb on - strand, within PP_0235 at 290.471 kb on + strand, within PP_0235 at 290.472 kb on - strand, within PP_0235 at 290.493 kb on - strand, within PP_0235 at 290.493 kb on - strand, within PP_0235 at 290.688 kb on + strand at 290.689 kb on - strand at 290.745 kb on - strand at 290.903 kb on + strand at 290.904 kb on - strand at 291.294 kb on + strand, within PP_0236 at 291.406 kb on + strand, within PP_0236 at 291.407 kb on - strand, within PP_0236 at 291.407 kb on - strand, within PP_0236 at 291.558 kb on + strand at 291.794 kb on + strand, within PP_0237 at 291.795 kb on - strand, within PP_0237 at 291.795 kb on - strand, within PP_0237 at 291.925 kb on + strand, within PP_0237 at 291.925 kb on + strand, within PP_0237 at 291.926 kb on - strand, within PP_0237 at 291.926 kb on - strand, within PP_0237 at 291.926 kb on - strand, within PP_0237 at 291.926 kb on - strand, within PP_0237 at 291.926 kb on - strand, within PP_0237 at 291.963 kb on + strand, within PP_0237 at 291.963 kb on + strand, within PP_0237 at 292.051 kb on + strand, within PP_0237 at 292.051 kb on + strand, within PP_0237 at 292.051 kb on + strand, within PP_0237 at 292.052 kb on - strand, within PP_0237 at 292.052 kb on - strand, within PP_0237 at 292.090 kb on + strand, within PP_0237 at 292.111 kb on + strand, within PP_0237 at 292.112 kb on - strand, within PP_0237 at 292.112 kb on - strand, within PP_0237 at 292.261 kb on + strand, within PP_0237 at 292.279 kb on + strand, within PP_0237 at 292.280 kb on - strand, within PP_0237 at 292.280 kb on - strand, within PP_0237 at 292.280 kb on - strand, within PP_0237 at 292.439 kb on - strand, within PP_0237 at 292.518 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 289,974 - -0.9 290,048 + +0.4 290,048 + +0.8 290,049 - +1.3 290,049 - +0.6 290,348 + PP_0235 0.42 +2.6 290,349 - PP_0235 0.42 +2.6 290,404 - PP_0235 0.50 +2.6 290,404 - PP_0235 0.50 -0.2 290,471 + PP_0235 0.61 -0.6 290,472 - PP_0235 0.61 -0.1 290,493 - PP_0235 0.64 -1.3 290,493 - PP_0235 0.64 -0.3 290,688 + +0.8 290,689 - +0.7 290,745 - -1.7 290,903 + +0.0 290,904 - +0.9 291,294 + PP_0236 0.60 -0.2 291,406 + PP_0236 0.78 -0.4 291,407 - PP_0236 0.79 -1.0 291,407 - PP_0236 0.79 +0.3 291,558 + -0.3 291,794 + PP_0237 0.18 -0.9 291,795 - PP_0237 0.18 +0.1 291,795 - PP_0237 0.18 -0.9 291,925 + PP_0237 0.32 +1.6 291,925 + PP_0237 0.32 -2.0 291,926 - PP_0237 0.32 +0.3 291,926 - PP_0237 0.32 -1.1 291,926 - PP_0237 0.32 -0.1 291,926 - PP_0237 0.32 -0.3 291,926 - PP_0237 0.32 +0.5 291,963 + PP_0237 0.36 -0.3 291,963 + PP_0237 0.36 +0.1 292,051 + PP_0237 0.45 +0.1 292,051 + PP_0237 0.45 -0.1 292,051 + PP_0237 0.45 -0.1 292,052 - PP_0237 0.45 +2.3 292,052 - PP_0237 0.45 +0.3 292,090 + PP_0237 0.49 +0.3 292,111 + PP_0237 0.51 +0.1 292,112 - PP_0237 0.51 +0.3 292,112 - PP_0237 0.51 +0.4 292,261 + PP_0237 0.66 +0.3 292,279 + PP_0237 0.68 +0.0 292,280 - PP_0237 0.68 -0.9 292,280 - PP_0237 0.68 +0.3 292,280 - PP_0237 0.68 +1.6 292,439 - PP_0237 0.85 -1.4 292,518 - -0.3
Or see this region's nucleotide sequence