Strain Fitness in Pseudomonas putida KT2440 around PP_0086

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
94,142	+		PP_0084	0.49	+0.1
94,142	+		PP_0084	0.49	+0.6
94,143	-		PP_0084	0.49	+0.1
94,143	-		PP_0084	0.49	+0.2
94,358	+		PP_0084	0.73	-0.2
94,358	+		PP_0084	0.73	+0.1
94,358	+		PP_0084	0.73	-0.3
94,382	+		PP_0084	0.76	-2.4
94,383	-		PP_0084	0.76	-1.1
94,383	-		PP_0084	0.76	-1.3
94,383	-		PP_0084	0.76	+0.1
94,383	-		PP_0084	0.76	-0.8
94,383	-		PP_0084	0.76	+2.6
94,383	-		PP_0084	0.76	-0.9
94,385	+		PP_0084	0.76	-0.6
94,386	-		PP_0084	0.76	+0.4
94,386	-		PP_0084	0.76	+0.6
94,386	-		PP_0084	0.76	-0.8
94,386	-		PP_0084	0.76	-0.6
94,495	+		PP_0084	0.88	+0.2
94,496	-		PP_0084	0.88	-1.3
94,599	-				-0.2
94,612	+				-0.7
94,613	-				+2.0
94,619	+				-0.3
94,667	+		PP_0085	0.14	-0.5
94,667	+		PP_0085	0.14	-1.0
94,667	+		PP_0085	0.14	+1.1
94,667	+		PP_0085	0.14	-0.0
94,668	-		PP_0085	0.15	+1.6
94,668	-		PP_0085	0.15	+0.1
94,736	+		PP_0085	0.28	+0.1
94,737	-		PP_0085	0.29	+0.0
94,750	+		PP_0085	0.31	+0.8
94,900	-		PP_0085	0.62	-0.8
94,900	-		PP_0085	0.62	-2.6
94,939	+		PP_0085	0.70	+1.8
94,940	-		PP_0085	0.70	+0.1
94,940	-		PP_0085	0.70	+0.4
95,016	+		PP_0085	0.86	+0.1
95,017	-		PP_0085	0.86	-1.9
95,035	+		PP_0085	0.90	-0.2
95,036	-		PP_0085	0.90	+0.3
95,089	+				+0.2
95,090	-				-0.3
95,142	+		PP_0086	0.33	-1.3
95,142	+		PP_0086	0.33	+0.1
95,142	+		PP_0086	0.33	-1.8
95,280	+				-1.4
95,280	+				+0.1
95,280	+				-1.2
95,302	+				-0.9
95,303	-				+0.9
95,303	-				-1.3
95,303	-				-0.2
95,303	-				+0.0
95,303	-				+1.1
95,323	+				-0.1
95,324	-				+0.8
95,514	-		PP_0087	0.64	+2.1
95,563	+		PP_0087	0.83	-0.1
95,563	+		PP_0087	0.83	+0.1
95,564	-		PP_0087	0.83	-0.4
95,606	+				+2.6
95,607	-				+0.1
95,651	+				-0.7
95,651	+				+0.7
95,651	+				-0.9
95,651	+				-0.6
95,651	+				+1.6
95,651	+				+0.3
95,652	-				+0.7
95,652	-				-0.2
95,714	+				+0.6
95,714	+				+0.1
95,714	+				-0.4
95,714	+				-0.2
95,715	-				+0.2
95,741	+				+2.6
95,741	+				-0.1
95,741	+				+0.6
95,742	-				-0.2
95,742	-				-0.3
95,742	-				+1.1
95,742	-				+1.0
95,754	+				-0.8
95,754	+				-0.4
95,755	-				-1.4
95,755	-				+0.6
95,797	+		PP_0088	0.12	+0.3
95,798	-		PP_0088	0.12	+0.1
95,798	-		PP_0088	0.12	-0.3
95,798	-		PP_0088	0.12	+1.1
95,809	+		PP_0088	0.13	+1.4
95,810	-		PP_0088	0.13	+2.6
95,810	-		PP_0088	0.13	+0.1
95,955	-		PP_0088	0.27	-0.1
95,973	-		PP_0088	0.29	+0.5
95,973	-		PP_0088	0.29	-1.7
96,026	+		PP_0088	0.34	+1.1
96,026	+		PP_0088	0.34	-0.1
96,026	+		PP_0088	0.34	-1.4
96,026	+		PP_0088	0.34	+0.1
96,026	+		PP_0088	0.34	-0.0
96,093	-		PP_0088	0.41	-0.4
96,123	-		PP_0088	0.44	+1.4
96,200	+		PP_0088	0.52	+0.3
96,249	-		PP_0088	0.57	+1.6