Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5136 and Atu5137 are separated by 55 nucleotides Atu5137 and Atu5138 are separated by 20 nucleotides
Atu5136: Atu5136 - transcriptional repressor of the blcABC operon, at 142,352 to 143,179
Atu5136
Atu5137: Atu5137 - NAD-dependent succinyl-semialdehyde dehydrogenase, at 143,235 to 144,689
Atu5137
Atu5138: Atu5138 - gamma hydroxybutyrate dehydrogenase, at 144,710 to 145,870
Atu5138
Position (kb)
143
144
145 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 142.255 kb on + strand at 142.255 kb on + strand at 142.255 kb on + strand at 142.255 kb on + strand at 142.261 kb on - strand at 142.263 kb on + strand at 142.263 kb on - strand at 142.263 kb on - strand at 142.263 kb on - strand at 142.263 kb on - strand at 142.329 kb on + strand at 142.332 kb on - strand at 142.344 kb on - strand at 142.419 kb on - strand at 142.465 kb on - strand, within Atu5136 at 142.526 kb on - strand, within Atu5136 at 142.546 kb on - strand, within Atu5136 at 142.634 kb on - strand, within Atu5136 at 142.634 kb on - strand, within Atu5136 at 142.636 kb on + strand, within Atu5136 at 142.645 kb on - strand, within Atu5136 at 142.680 kb on - strand, within Atu5136 at 142.815 kb on - strand, within Atu5136 at 142.821 kb on - strand, within Atu5136 at 143.046 kb on - strand, within Atu5136 at 143.139 kb on + strand at 143.139 kb on + strand at 143.147 kb on - strand at 143.157 kb on - strand at 143.277 kb on - strand at 143.299 kb on - strand at 143.384 kb on + strand, within Atu5137 at 143.473 kb on + strand, within Atu5137 at 143.473 kb on + strand, within Atu5137 at 143.473 kb on + strand, within Atu5137 at 143.513 kb on + strand, within Atu5137 at 143.516 kb on + strand, within Atu5137 at 143.516 kb on + strand, within Atu5137 at 143.521 kb on + strand, within Atu5137 at 143.563 kb on - strand, within Atu5137 at 143.869 kb on - strand, within Atu5137 at 143.911 kb on - strand, within Atu5137 at 143.913 kb on + strand, within Atu5137 at 143.943 kb on + strand, within Atu5137 at 144.028 kb on + strand, within Atu5137 at 144.063 kb on + strand, within Atu5137 at 144.093 kb on + strand, within Atu5137 at 144.138 kb on + strand, within Atu5137 at 144.172 kb on - strand, within Atu5137 at 144.277 kb on + strand, within Atu5137 at 144.277 kb on + strand, within Atu5137 at 144.351 kb on + strand, within Atu5137 at 144.369 kb on - strand, within Atu5137 at 144.393 kb on - strand, within Atu5137 at 144.397 kb on + strand, within Atu5137 at 144.398 kb on + strand, within Atu5137 at 144.405 kb on - strand, within Atu5137 at 144.554 kb on + strand at 144.559 kb on + strand at 144.565 kb on - strand at 144.567 kb on - strand at 144.608 kb on + strand at 144.620 kb on + strand at 144.627 kb on + strand at 144.635 kb on + strand at 144.668 kb on + strand at 144.699 kb on + strand at 144.747 kb on - strand at 144.801 kb on + strand at 144.809 kb on - strand at 144.823 kb on + strand at 144.844 kb on + strand, within Atu5138 at 144.847 kb on - strand, within Atu5138 at 144.852 kb on - strand, within Atu5138 at 144.852 kb on - strand, within Atu5138 at 144.852 kb on - strand, within Atu5138 at 144.872 kb on + strand, within Atu5138 at 144.974 kb on - strand, within Atu5138 at 145.093 kb on + strand, within Atu5138 at 145.216 kb on + strand, within Atu5138 at 145.216 kb on + strand, within Atu5138 at 145.216 kb on + strand, within Atu5138 at 145.237 kb on + strand, within Atu5138 at 145.270 kb on + strand, within Atu5138 at 145.270 kb on + strand, within Atu5138 at 145.273 kb on + strand, within Atu5138 at 145.319 kb on + strand, within Atu5138 at 145.382 kb on - strand, within Atu5138 at 145.389 kb on + strand, within Atu5138 at 145.397 kb on - strand, within Atu5138 at 145.408 kb on + strand, within Atu5138 at 145.462 kb on + strand, within Atu5138 at 145.477 kb on - strand, within Atu5138 at 145.477 kb on - strand, within Atu5138 at 145.477 kb on - strand, within Atu5138 at 145.562 kb on + strand, within Atu5138 at 145.563 kb on + strand, within Atu5138
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 142,255 + -0.0 142,255 + +0.3 142,255 + -0.5 142,255 + +1.9 142,261 - +0.3 142,263 + +1.2 142,263 - -0.3 142,263 - -1.4 142,263 - -0.7 142,263 - -0.2 142,329 + +1.0 142,332 - +0.8 142,344 - -0.5 142,419 - -1.1 142,465 - Atu5136 0.14 -0.5 142,526 - Atu5136 0.21 -0.8 142,546 - Atu5136 0.23 -0.8 142,634 - Atu5136 0.34 -1.2 142,634 - Atu5136 0.34 +0.7 142,636 + Atu5136 0.34 -1.2 142,645 - Atu5136 0.35 -0.8 142,680 - Atu5136 0.40 -1.3 142,815 - Atu5136 0.56 -1.9 142,821 - Atu5136 0.57 -2.6 143,046 - Atu5136 0.84 -3.1 143,139 + +1.7 143,139 + +0.5 143,147 - -0.8 143,157 - -0.1 143,277 - +2.3 143,299 - -0.4 143,384 + Atu5137 0.10 +0.4 143,473 + Atu5137 0.16 +1.2 143,473 + Atu5137 0.16 +0.5 143,473 + Atu5137 0.16 -1.1 143,513 + Atu5137 0.19 -1.2 143,516 + Atu5137 0.19 -0.5 143,516 + Atu5137 0.19 +0.1 143,521 + Atu5137 0.20 -0.5 143,563 - Atu5137 0.23 -0.2 143,869 - Atu5137 0.44 -0.9 143,911 - Atu5137 0.46 -2.2 143,913 + Atu5137 0.47 +0.0 143,943 + Atu5137 0.49 -1.4 144,028 + Atu5137 0.55 +0.4 144,063 + Atu5137 0.57 +0.4 144,093 + Atu5137 0.59 +0.1 144,138 + Atu5137 0.62 -0.9 144,172 - Atu5137 0.64 +0.3 144,277 + Atu5137 0.72 -0.0 144,277 + Atu5137 0.72 -0.9 144,351 + Atu5137 0.77 -0.5 144,369 - Atu5137 0.78 -0.4 144,393 - Atu5137 0.80 -1.2 144,397 + Atu5137 0.80 +0.2 144,398 + Atu5137 0.80 -0.4 144,405 - Atu5137 0.80 +0.3 144,554 + +0.2 144,559 + -0.4 144,565 - -1.1 144,567 - -0.7 144,608 + +0.1 144,620 + +1.2 144,627 + +0.6 144,635 + +0.0 144,668 + -0.9 144,699 + -0.6 144,747 - -1.2 144,801 + -0.4 144,809 - -1.0 144,823 + +0.3 144,844 + Atu5138 0.12 -0.4 144,847 - Atu5138 0.12 +0.3 144,852 - Atu5138 0.12 -0.4 144,852 - Atu5138 0.12 +0.4 144,852 - Atu5138 0.12 -0.2 144,872 + Atu5138 0.14 -0.5 144,974 - Atu5138 0.23 -0.8 145,093 + Atu5138 0.33 +0.2 145,216 + Atu5138 0.44 -0.1 145,216 + Atu5138 0.44 +0.8 145,216 + Atu5138 0.44 -0.5 145,237 + Atu5138 0.45 +1.6 145,270 + Atu5138 0.48 -0.4 145,270 + Atu5138 0.48 +0.0 145,273 + Atu5138 0.48 +0.2 145,319 + Atu5138 0.52 +0.2 145,382 - Atu5138 0.58 -1.2 145,389 + Atu5138 0.58 +1.7 145,397 - Atu5138 0.59 -1.4 145,408 + Atu5138 0.60 +1.9 145,462 + Atu5138 0.65 -0.4 145,477 - Atu5138 0.66 +0.1 145,477 - Atu5138 0.66 +0.1 145,477 - Atu5138 0.66 +0.3 145,562 + Atu5138 0.73 +0.8 145,563 + Atu5138 0.73 -1.6
Or see this region's nucleotide sequence