Strain Fitness in Pseudomonas putida KT2440 around PP_5608

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
4,095,451	+				+1.2
4,095,476	+				-0.5
4,095,505	+				+0.8
4,095,521	-				-0.5
4,095,530	+				+0.0
4,095,530	+				+0.5
4,095,531	-				-2.3
4,095,548	+				-1.0
4,095,548	+				-0.1
4,095,548	+				-1.2
4,095,548	+				+0.5
4,095,549	-				+0.7
4,095,549	-				-0.6
4,095,549	-				-0.5
4,095,549	-				+0.1
4,095,549	-				-0.7
4,095,549	-				+1.1
4,095,623	+				+0.2
4,095,623	+				-0.8
4,095,623	+				-0.5
4,095,624	-				+0.1
4,095,628	+				+0.5
4,095,628	+				+0.3
4,095,628	+				+0.5
4,095,629	-				-1.7
4,095,629	-				+0.9
4,095,629	-				+1.3
4,095,669	-				-0.2
4,095,765	+		PP_5607	0.15	+0.2
4,095,765	+		PP_5607	0.15	-0.5
4,095,765	+		PP_5607	0.15	-0.4
4,095,766	-		PP_5607	0.15	-0.3
4,095,853	+		PP_5607	0.28	-1.8
4,095,853	+		PP_5607	0.28	-0.3
4,096,082	-		PP_5607	0.63	+0.9
4,096,093	+		PP_5607	0.65	-0.3
4,096,094	-		PP_5607	0.65	+0.4
4,096,215	+		PP_5607	0.84	+0.6
4,096,215	+		PP_5607	0.84	+0.2
4,096,226	+		PP_5607	0.86	-0.3
4,096,227	-		PP_5607	0.86	+0.4
4,096,227	-		PP_5607	0.86	+1.6
4,096,227	-		PP_5607	0.86	+1.1
4,096,230	+		PP_5607	0.86	-0.1
4,096,230	+		PP_5607	0.86	-0.8
4,096,230	+		PP_5607	0.86	+0.4
4,096,231	-		PP_5607	0.86	-0.6
4,096,231	-		PP_5607	0.86	+0.2
4,096,231	-		PP_5607	0.86	+0.8
4,096,383	-				-0.6
4,096,407	+				+0.9
4,096,554	-		PP_5608	0.48	-0.8
4,096,554	-		PP_5608	0.48	-0.3
4,096,579	-		PP_5608	0.56	-1.2
4,096,637	+		PP_5608	0.74	-0.5
4,096,637	+		PP_5608	0.74	-1.3
4,096,637	+		PP_5608	0.74	-1.1
4,096,682	+		PP_5608	0.87	-0.6
4,096,682	+		PP_5608	0.87	-0.3
4,096,721	+				-0.1
4,096,729	+				-0.4
4,096,729	+				-0.4
4,096,729	+				-0.1
4,096,729	+				-0.4
4,096,730	-				-1.5
4,096,730	-				-0.5
4,096,730	-				-0.0
4,096,730	-				-0.8
4,096,734	-				-1.1
4,096,855	+		PP_3605	0.11	-1.4
4,096,855	+		PP_3605	0.11	+0.6
4,096,856	-		PP_3605	0.11	-1.2
4,096,856	-		PP_3605	0.11	-0.1
4,097,092	-		PP_3605	0.37	-0.4
4,097,149	+		PP_3605	0.43	-0.4
4,097,149	+		PP_3605	0.43	-1.0
4,097,192	+		PP_3605	0.47	-0.3
4,097,282	-		PP_3605	0.57	-0.2
4,097,309	-		PP_3605	0.60	-0.3
4,097,316	+		PP_3605	0.61	+1.1
4,097,542	-		PP_3605	0.85	-0.1
4,097,570	+		PP_3605	0.88	+0.2
4,097,571	-		PP_3605	0.89	+1.5
4,097,571	-		PP_3605	0.89	-1.8
4,097,571	-		PP_3605	0.89	+0.3
4,097,571	-		PP_3605	0.89	-0.4
4,097,571	-		PP_3605	0.89	+0.1
4,097,571	-		PP_3605	0.89	-0.1
4,097,620	+				+0.8
4,097,620	+				-1.2
4,097,620	+				-0.1
4,097,620	+				+0.2
4,097,620	+				-0.5
4,097,621	-				+2.5
4,097,621	-				-0.0
4,097,621	-				+1.2
4,097,621	-				+0.0
4,097,669	+				-0.3
4,097,669	+				-0.2
4,097,670	-				-0.6