Strain Fitness in Pseudomonas putida KT2440 around PP_5359

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
6,107,821	+		PP_5357	0.62	-0.6
6,107,821	+		PP_5357	0.62	+1.4
6,107,821	+		PP_5357	0.62	+0.2
6,107,822	-		PP_5357	0.62	-0.2
6,107,822	-		PP_5357	0.62	-1.6
6,107,822	-		PP_5357	0.62	-0.1
6,107,822	-		PP_5357	0.62	-1.2
6,107,822	-		PP_5357	0.62	-0.1
6,107,822	-		PP_5357	0.62	-1.0
6,107,823	+		PP_5357	0.62	-2.0
6,107,823	+		PP_5357	0.62	+0.3
6,107,823	+		PP_5357	0.62	-1.8
6,107,824	-		PP_5357	0.62	+0.6
6,108,007	+		PP_5357	0.83	-0.2
6,108,008	-		PP_5357	0.83	-1.0
6,108,036	-		PP_5357	0.87	+0.9
6,108,143	+				+0.5
6,108,177	+				-0.4
6,108,178	-				-0.0
6,108,198	-				-0.7
6,108,198	-				+1.2
6,108,212	+				-0.5
6,108,213	-				-0.6
6,108,213	-				-1.0
6,108,213	-				+0.8
6,108,213	-				-1.1
6,108,213	-				+0.9
6,108,218	+				-1.1
6,108,219	-				+0.2
6,108,252	-				-0.4
6,108,252	-				+0.3
6,108,266	+				-0.1
6,108,266	+				-0.2
6,108,266	+				+0.2
6,108,266	+				+0.0
6,108,266	+				-0.0
6,108,267	-				-0.6
6,108,267	-				+3.4
6,108,267	-				+1.2
6,108,267	-				+0.4
6,108,267	-				+1.0
6,108,311	-		PP_5358	0.11	-0.2
6,108,311	-		PP_5358	0.11	+0.7
6,108,422	-		PP_5358	0.39	-1.8
6,108,434	+		PP_5358	0.42	+0.1
6,108,483	+		PP_5358	0.54	-0.2
6,108,483	+		PP_5358	0.54	-0.6
6,108,484	-		PP_5358	0.54	+1.4
6,108,484	-		PP_5358	0.54	+0.0
6,108,484	-		PP_5358	0.54	+0.8
6,108,568	+		PP_5358	0.75	+0.5
6,108,569	-		PP_5358	0.76	-0.2
6,108,576	+		PP_5358	0.77	+0.2
6,108,577	-		PP_5358	0.78	+0.8
6,108,619	+		PP_5358	0.88	-0.4
6,108,620	-		PP_5358	0.88	+0.2
6,108,620	-		PP_5358	0.88	-0.9
6,108,620	-		PP_5358	0.88	-0.2
6,108,620	-		PP_5358	0.88	+0.1
6,108,620	-		PP_5358	0.88	+0.3
6,108,782	+		PP_5359	0.12	-1.7
6,109,485	+		PP_5359	0.84	-0.5
6,109,528	-		PP_5359	0.89	-1.3
6,109,611	+				+0.4
6,109,611	+				-0.6
6,109,611	+				-0.2
6,109,612	-				-1.2
6,109,612	-				-0.1
6,109,612	-				+0.8
6,109,669	+				+1.6
6,109,669	+				+0.4
6,109,670	-				+1.6
6,109,779	+		PP_5360	0.16	-0.9
6,109,779	+		PP_5360	0.16	+0.6
6,109,780	-		PP_5360	0.16	+0.3
6,109,780	-		PP_5360	0.16	-0.2
6,109,780	-		PP_5360	0.16	-1.4
6,109,780	-		PP_5360	0.16	-0.0
6,109,783	+		PP_5360	0.17	+0.2
6,109,783	+		PP_5360	0.17	-0.2
6,109,784	-		PP_5360	0.17	-0.4
6,109,843	+		PP_5360	0.26	-0.7
6,109,843	+		PP_5360	0.26	+0.2
6,109,861	-		PP_5360	0.29	-0.5
6,109,884	+		PP_5360	0.33	-0.8
6,109,885	-		PP_5360	0.33	+0.6
6,109,885	-		PP_5360	0.33	-0.4
6,109,898	+		PP_5360	0.35	-1.2
6,109,898	+		PP_5360	0.35	+0.1
6,109,898	+		PP_5360	0.35	+0.6
6,109,899	-		PP_5360	0.35	-1.2
6,109,899	-		PP_5360	0.35	-1.0
6,109,921	+		PP_5360	0.39	-0.9
6,109,922	-		PP_5360	0.39	+0.3
6,109,922	-		PP_5360	0.39	+0.4
6,110,007	+		PP_5360	0.52	+0.2
6,110,007	+		PP_5360	0.52	-0.1
6,110,007	+		PP_5360	0.52	-0.3
6,110,007	+		PP_5360	0.52	-1.9
6,110,007	+		PP_5360	0.52	-1.5
6,110,007	+		PP_5360	0.52	+3.1
6,110,007	+		PP_5360	0.52	+0.1
6,110,007	+		PP_5360	0.52	+0.5
6,110,007	+		PP_5360	0.52	-0.5
6,110,007	+		PP_5360	0.52	-0.2
6,110,007	+		PP_5360	0.52	+0.5
6,110,007	+		PP_5360	0.52	-0.4
6,110,008	-		PP_5360	0.53	+0.0
6,110,008	-		PP_5360	0.53	-1.2
6,110,009	+		PP_5360	0.53	+0.8
6,110,009	+		PP_5360	0.53	+0.4
6,110,009	+		PP_5360	0.53	-0.6
6,110,009	+		PP_5360	0.53	-0.5
6,110,009	+		PP_5360	0.53	-0.6
6,110,009	+		PP_5360	0.53	+0.8
6,110,009	+		PP_5360	0.53	+0.5
6,110,010	-		PP_5360	0.53	-0.8
6,110,010	-		PP_5360	0.53	-0.2
6,110,010	-		PP_5360	0.53	-1.2
6,110,010	-		PP_5360	0.53	-0.4
6,110,010	-		PP_5360	0.53	+0.2
6,110,038	+		PP_5360	0.57	+0.8
6,110,039	-		PP_5360	0.58	+1.0
6,110,039	-		PP_5360	0.58	+0.6
6,110,039	-		PP_5360	0.58	+0.2
6,110,051	+		PP_5360	0.59	-0.0
6,110,052	-		PP_5360	0.60	-1.0
6,110,052	-		PP_5360	0.60	+0.5
6,110,343	+				-0.4
6,110,343	+				-0.6
6,110,444	+		PP_5361	0.12	+0.3
6,110,445	-		PP_5361	0.12	-0.4
6,110,617	+		PP_5361	0.26	-1.1
6,110,617	+		PP_5361	0.26	-0.7
6,110,618	-		PP_5361	0.26	-0.7