Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5046 and PP_5047 are separated by 365 nucleotides PP_5047 and PP_5048 overlap by 4 nucleotides
PP_5046: PP_5046 - glutamine synthetase, at 5,752,033 to 5,753,439
_5046
PP_5047: PP_5047 - two-component system sensory histidine kinase/phosphatase GlnL/GlnG, at 5,753,805 to 5,754,890
_5047
PP_5048: PP_5048 - two-component system DNA-binding transcriptional dual regulator GlnL/GlnG, at 5,754,887 to 5,756,323
_5048
Position (kb) 5753
5754
5755 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5753.546 kb on + strand at 5753.662 kb on - strand at 5753.762 kb on - strand at 5753.804 kb on + strand at 5753.804 kb on + strand at 5753.804 kb on + strand at 5753.892 kb on + strand at 5753.892 kb on + strand at 5753.892 kb on + strand at 5753.893 kb on - strand at 5754.050 kb on + strand, within PP_5047 at 5754.051 kb on - strand, within PP_5047 at 5754.092 kb on - strand, within PP_5047 at 5754.274 kb on + strand, within PP_5047 at 5754.312 kb on + strand, within PP_5047 at 5754.313 kb on - strand, within PP_5047 at 5754.313 kb on - strand, within PP_5047 at 5754.407 kb on + strand, within PP_5047 at 5754.729 kb on + strand, within PP_5047 at 5754.729 kb on + strand, within PP_5047 at 5754.729 kb on + strand, within PP_5047 at 5754.729 kb on + strand, within PP_5047 at 5754.730 kb on - strand, within PP_5047 at 5754.779 kb on + strand, within PP_5047 at 5754.855 kb on + strand at 5754.855 kb on + strand at 5754.855 kb on + strand at 5754.856 kb on - strand at 5754.856 kb on - strand at 5754.856 kb on - strand at 5754.907 kb on - strand at 5755.169 kb on + strand, within PP_5048 at 5755.170 kb on - strand, within PP_5048 at 5755.170 kb on - strand, within PP_5048 at 5755.170 kb on - strand, within PP_5048 at 5755.170 kb on - strand, within PP_5048 at 5755.190 kb on + strand, within PP_5048 at 5755.674 kb on - strand, within PP_5048 at 5755.805 kb on - strand, within PP_5048 at 5755.810 kb on + strand, within PP_5048 at 5755.811 kb on - strand, within PP_5048 at 5755.811 kb on - strand, within PP_5048 at 5755.811 kb on - strand, within PP_5048
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. remove 5,753,546 + -1.2 5,753,662 - +1.1 5,753,762 - -0.5 5,753,804 + +0.2 5,753,804 + -0.3 5,753,804 + -1.6 5,753,892 + -0.8 5,753,892 + -0.9 5,753,892 + -1.3 5,753,893 - +0.2 5,754,050 + PP_5047 0.23 -0.2 5,754,051 - PP_5047 0.23 -1.1 5,754,092 - PP_5047 0.26 -2.0 5,754,274 + PP_5047 0.43 -0.6 5,754,312 + PP_5047 0.47 -0.6 5,754,313 - PP_5047 0.47 -1.3 5,754,313 - PP_5047 0.47 -1.6 5,754,407 + PP_5047 0.55 -1.7 5,754,729 + PP_5047 0.85 -1.1 5,754,729 + PP_5047 0.85 -0.9 5,754,729 + PP_5047 0.85 -1.8 5,754,729 + PP_5047 0.85 -1.7 5,754,730 - PP_5047 0.85 -2.0 5,754,779 + PP_5047 0.90 -1.5 5,754,855 + -0.2 5,754,855 + -1.1 5,754,855 + +2.4 5,754,856 - +2.4 5,754,856 - -0.7 5,754,856 - -0.4 5,754,907 - +0.0 5,755,169 + PP_5048 0.20 -1.3 5,755,170 - PP_5048 0.20 -2.1 5,755,170 - PP_5048 0.20 -1.0 5,755,170 - PP_5048 0.20 -1.1 5,755,170 - PP_5048 0.20 -0.7 5,755,190 + PP_5048 0.21 +0.2 5,755,674 - PP_5048 0.55 -1.5 5,755,805 - PP_5048 0.64 +0.0 5,755,810 + PP_5048 0.64 -0.5 5,755,811 - PP_5048 0.64 -1.7 5,755,811 - PP_5048 0.64 +2.4 5,755,811 - PP_5048 0.64 +0.1
Or see this region's nucleotide sequence