Strain Fitness in Pseudomonas putida KT2440 around PP_3956

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
4,462,643	-		PP_3955	0.56	-1.4
4,462,897	+		PP_3955	0.68	-0.1
4,462,942	-		PP_3955	0.70	+0.4
4,462,942	-		PP_3955	0.70	-0.6
4,463,008	+		PP_3955	0.74	+0.8
4,463,008	+		PP_3955	0.74	+0.6
4,463,008	+		PP_3955	0.74	-0.0
4,463,009	-		PP_3955	0.74	-0.5
4,463,040	+		PP_3955	0.75	-0.3
4,463,040	+		PP_3955	0.75	-0.3
4,463,040	+		PP_3955	0.75	-0.1
4,463,040	+		PP_3955	0.75	-0.4
4,463,041	-		PP_3955	0.75	-1.4
4,463,041	-		PP_3955	0.75	-0.3
4,463,041	-		PP_3955	0.75	+2.4
4,463,041	-		PP_3955	0.75	-0.2
4,463,041	-		PP_3955	0.75	-0.0
4,463,122	-		PP_3955	0.79	+0.1
4,463,269	-		PP_3955	0.87	-1.3
4,463,269	-		PP_3955	0.87	-0.4
4,463,269	-		PP_3955	0.87	+1.1
4,463,316	+		PP_3955	0.89	+0.3
4,463,316	+		PP_3955	0.89	+0.3
4,463,316	+		PP_3955	0.89	+0.8
4,463,316	+		PP_3955	0.89	-0.5
4,463,317	-		PP_3955	0.89	+0.2
4,463,317	-		PP_3955	0.89	+0.1
4,463,317	-		PP_3955	0.89	-0.3
4,463,317	-		PP_3955	0.89	-0.7
4,463,317	-		PP_3955	0.89	-0.3
4,463,317	-		PP_3955	0.89	-0.2
4,463,437	+				+0.5
4,463,437	+				-0.9
4,463,502	+				+0.1
4,463,600	-		PP_3956	0.15	+0.1
4,463,649	-		PP_3956	0.27	-1.4
4,463,649	-		PP_3956	0.27	-0.4
4,463,649	-		PP_3956	0.27	-1.1
4,463,673	+		PP_3956	0.33	-0.8
4,463,769	+		PP_3956	0.56	+0.1
4,463,769	+		PP_3956	0.56	-0.9
4,463,770	-		PP_3956	0.56	-1.1
4,463,858	+		PP_3956	0.77	-0.8
4,463,859	-		PP_3956	0.77	+0.8
4,463,866	-		PP_3956	0.79	-0.2
4,463,866	-		PP_3956	0.79	+0.4
4,463,895	+		PP_3956	0.86	-0.6
4,464,000	+				-0.1
4,464,000	+				+0.5
4,464,000	+				+1.3
4,464,000	+				+0.8
4,464,001	-				+0.5
4,464,001	-				+1.1
4,464,001	-				-1.2
4,464,018	+				-0.1
4,464,019	-				-0.7
4,464,019	-				+1.3
4,464,019	-				+0.2
4,464,115	-				+0.7
4,464,166	+				+0.4
4,464,167	-				+1.4
4,464,193	-		PP_3957	0.11	-0.7
4,464,193	-		PP_3957	0.11	-1.9
4,464,233	+		PP_3957	0.13	+0.2
4,464,233	+		PP_3957	0.13	+0.2
4,464,234	-		PP_3957	0.13	+0.1
4,464,332	-		PP_3957	0.18	-0.4
4,464,371	-		PP_3957	0.20	-0.4
4,464,596	+		PP_3957	0.31	-0.7
4,464,596	+		PP_3957	0.31	+0.8
4,464,839	-		PP_3957	0.43	+0.4
4,464,839	-		PP_3957	0.43	+0.7
4,464,839	-		PP_3957	0.43	+0.4
4,464,943	+		PP_3957	0.49	+0.6
4,464,944	-		PP_3957	0.49	+2.4
4,464,944	-		PP_3957	0.49	-1.0
4,464,944	-		PP_3957	0.49	+0.1