Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3950 and PP_3951 are separated by 378 nucleotides PP_3951 and PP_3952 are separated by 8 nucleotides PP_3952 and PP_3953 are separated by 267 nucleotides
PP_3950: PP_3950 - Methyl-accepting chemotaxis transducer, at 4,454,807 to 4,456,984
_3950
PP_3951: PP_3951 - 3-oxoadipate CoA-transferase subunit A, at 4,457,363 to 4,458,058
_3951
PP_3952: PP_3952 - 3-oxoadipate CoA-transferase subunit A, at 4,458,067 to 4,458,708
_3952
PP_3953: PP_3953 - potassium transporter, at 4,458,976 to 4,460,430
_3953
Position (kb) 4457
4458
4459 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4456.476 kb on + strand, within PP_3950 at 4456.476 kb on + strand, within PP_3950 at 4456.477 kb on - strand, within PP_3950 at 4456.477 kb on - strand, within PP_3950 at 4456.477 kb on - strand, within PP_3950 at 4456.477 kb on - strand, within PP_3950 at 4456.548 kb on + strand, within PP_3950 at 4456.548 kb on + strand, within PP_3950 at 4456.549 kb on - strand, within PP_3950 at 4456.606 kb on + strand, within PP_3950 at 4456.607 kb on - strand, within PP_3950 at 4456.607 kb on - strand, within PP_3950 at 4456.607 kb on - strand, within PP_3950 at 4456.943 kb on + strand at 4456.944 kb on - strand at 4456.944 kb on - strand at 4456.944 kb on - strand at 4456.973 kb on + strand at 4456.973 kb on + strand at 4456.974 kb on - strand at 4456.974 kb on - strand at 4456.974 kb on - strand at 4456.984 kb on + strand at 4456.985 kb on - strand at 4456.985 kb on - strand at 4457.165 kb on - strand at 4457.244 kb on - strand at 4457.258 kb on + strand at 4457.259 kb on - strand at 4457.260 kb on + strand at 4457.378 kb on + strand at 4457.379 kb on - strand at 4457.616 kb on - strand, within PP_3951 at 4457.616 kb on - strand, within PP_3951 at 4457.616 kb on - strand, within PP_3951 at 4457.616 kb on - strand, within PP_3951 at 4457.792 kb on + strand, within PP_3951 at 4457.792 kb on + strand, within PP_3951 at 4457.792 kb on + strand, within PP_3951 at 4457.793 kb on - strand, within PP_3951 at 4457.793 kb on - strand, within PP_3951 at 4457.993 kb on - strand at 4458.139 kb on + strand, within PP_3952 at 4458.139 kb on + strand, within PP_3952 at 4458.140 kb on - strand, within PP_3952 at 4458.140 kb on - strand, within PP_3952 at 4458.140 kb on - strand, within PP_3952 at 4458.143 kb on + strand, within PP_3952 at 4458.144 kb on - strand, within PP_3952 at 4458.144 kb on - strand, within PP_3952 at 4458.144 kb on - strand, within PP_3952 at 4458.182 kb on - strand, within PP_3952 at 4458.182 kb on - strand, within PP_3952 at 4458.378 kb on - strand, within PP_3952 at 4458.455 kb on + strand, within PP_3952 at 4458.456 kb on - strand, within PP_3952 at 4458.557 kb on - strand, within PP_3952 at 4458.592 kb on + strand, within PP_3952 at 4458.593 kb on - strand, within PP_3952 at 4458.593 kb on - strand, within PP_3952 at 4458.593 kb on - strand, within PP_3952 at 4458.609 kb on - strand, within PP_3952 at 4458.721 kb on + strand at 4458.721 kb on + strand at 4458.753 kb on + strand at 4458.753 kb on + strand at 4458.753 kb on + strand at 4458.754 kb on - strand at 4458.754 kb on - strand at 4458.754 kb on - strand at 4458.801 kb on - strand at 4458.801 kb on - strand at 4458.806 kb on + strand at 4458.907 kb on - strand at 4458.944 kb on + strand at 4458.945 kb on - strand at 4459.011 kb on + strand at 4459.012 kb on - strand at 4459.012 kb on - strand at 4459.019 kb on + strand at 4459.019 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. remove 4,456,476 + PP_3950 0.77 -0.5 4,456,476 + PP_3950 0.77 -0.7 4,456,477 - PP_3950 0.77 -1.5 4,456,477 - PP_3950 0.77 +0.1 4,456,477 - PP_3950 0.77 +0.2 4,456,477 - PP_3950 0.77 -0.2 4,456,548 + PP_3950 0.80 +1.4 4,456,548 + PP_3950 0.80 +2.1 4,456,549 - PP_3950 0.80 +0.1 4,456,606 + PP_3950 0.83 -0.2 4,456,607 - PP_3950 0.83 +0.9 4,456,607 - PP_3950 0.83 -0.1 4,456,607 - PP_3950 0.83 -1.4 4,456,943 + +0.2 4,456,944 - +0.4 4,456,944 - -0.6 4,456,944 - -0.3 4,456,973 + -0.6 4,456,973 + -0.4 4,456,974 - +0.7 4,456,974 - -1.9 4,456,974 - -0.1 4,456,984 + +0.9 4,456,985 - +0.1 4,456,985 - +0.9 4,457,165 - -0.4 4,457,244 - +0.4 4,457,258 + -0.9 4,457,259 - -0.1 4,457,260 + -0.4 4,457,378 + +0.7 4,457,379 - +0.3 4,457,616 - PP_3951 0.36 +0.6 4,457,616 - PP_3951 0.36 -0.6 4,457,616 - PP_3951 0.36 -1.8 4,457,616 - PP_3951 0.36 -0.4 4,457,792 + PP_3951 0.62 +1.0 4,457,792 + PP_3951 0.62 +0.1 4,457,792 + PP_3951 0.62 -0.3 4,457,793 - PP_3951 0.62 +0.0 4,457,793 - PP_3951 0.62 -0.5 4,457,993 - +0.4 4,458,139 + PP_3952 0.11 -0.4 4,458,139 + PP_3952 0.11 -1.2 4,458,140 - PP_3952 0.11 -1.1 4,458,140 - PP_3952 0.11 +0.7 4,458,140 - PP_3952 0.11 -0.8 4,458,143 + PP_3952 0.12 +0.4 4,458,144 - PP_3952 0.12 +0.4 4,458,144 - PP_3952 0.12 -0.6 4,458,144 - PP_3952 0.12 +0.4 4,458,182 - PP_3952 0.18 +0.2 4,458,182 - PP_3952 0.18 -1.4 4,458,378 - PP_3952 0.48 -0.1 4,458,455 + PP_3952 0.60 -1.7 4,458,456 - PP_3952 0.61 -0.8 4,458,557 - PP_3952 0.76 -0.3 4,458,592 + PP_3952 0.82 -0.4 4,458,593 - PP_3952 0.82 -1.1 4,458,593 - PP_3952 0.82 -0.2 4,458,593 - PP_3952 0.82 -0.6 4,458,609 - PP_3952 0.84 -1.0 4,458,721 + -1.7 4,458,721 + -0.3 4,458,753 + -0.7 4,458,753 + +0.1 4,458,753 + +0.5 4,458,754 - +0.6 4,458,754 - +0.0 4,458,754 - +0.1 4,458,801 - +0.3 4,458,801 - -0.3 4,458,806 + -0.1 4,458,907 - +0.1 4,458,944 + +0.7 4,458,945 - +1.5 4,459,011 + -0.6 4,459,012 - +1.4 4,459,012 - -0.2 4,459,019 + +0.3 4,459,019 + -1.3
Or see this region's nucleotide sequence