Strain Fitness in Pseudomonas putida KT2440 around PP_2790

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
3,177,758	+				+0.1
3,177,758	+				+0.5
3,178,026	-		PP_2789	0.17	+0.6
3,178,026	-		PP_2789	0.17	+0.1
3,178,080	+		PP_2789	0.25	-0.6
3,178,081	-		PP_2789	0.26	+0.7
3,178,081	-		PP_2789	0.26	-1.2
3,178,092	+		PP_2789	0.27	-0.8
3,178,124	+		PP_2789	0.33	+0.3
3,178,125	-		PP_2789	0.33	+0.2
3,178,125	-		PP_2789	0.33	-0.3
3,178,153	+		PP_2789	0.37	-0.0
3,178,179	+		PP_2789	0.42	-0.3
3,178,289	-		PP_2789	0.60	-0.5
3,178,341	+		PP_2789	0.68	+1.5
3,178,342	-		PP_2789	0.69	-0.7
3,178,342	-		PP_2789	0.69	+0.9
3,178,428	+		PP_2789	0.83	+0.0
3,178,428	+		PP_2789	0.83	-0.7
3,178,429	-		PP_2789	0.83	-0.8
3,178,429	-		PP_2789	0.83	+0.2
3,178,429	-		PP_2789	0.83	-0.2
3,178,429	-		PP_2789	0.83	-0.8
3,178,429	-		PP_2789	0.83	-0.1
3,178,535	+				-0.1
3,178,536	-				-0.8
3,178,555	-				+0.3
3,178,623	+				+0.7
3,178,624	-				-0.3
3,178,661	+				-0.3
3,178,662	-				+2.5
3,178,823	+				+0.3
3,178,823	+				-1.8
3,178,926	-		PP_2790	0.10	-0.1
3,178,941	+		PP_2790	0.11	-0.2
3,178,942	-		PP_2790	0.11	+0.2
3,178,942	-		PP_2790	0.11	-0.2
3,178,942	-		PP_2790	0.11	+0.4
3,179,202	+		PP_2790	0.25	-0.2
3,179,202	+		PP_2790	0.25	-1.0
3,179,203	-		PP_2790	0.25	+0.7
3,179,820	-		PP_2790	0.56	-0.6
3,179,820	-		PP_2790	0.56	-0.7
3,179,820	-		PP_2790	0.56	-0.8
3,180,035	-		PP_2790	0.67	+0.1
3,180,336	+		PP_2790	0.83	+1.9
3,180,337	-		PP_2790	0.83	-0.1
3,180,390	-		PP_2790	0.85	+0.7
3,180,522	-				-0.3
3,180,556	+				-0.7
3,180,562	+				+0.4
3,180,717	+				-2.2
3,180,717	+				-0.4
3,180,718	-				+2.5
3,180,783	+				+0.3
3,180,911	+				-0.8
3,180,912	-				+0.6
3,181,088	-		PP_2791	0.25	-0.4
3,181,165	-		PP_2791	0.32	+0.3
3,181,165	-		PP_2791	0.32	-0.1
3,181,248	+				+2.5
3,181,248	+		PP_2791	0.39	-0.3
3,181,249	-		PP_2791	0.39	-0.4
3,181,249	-		PP_2791	0.39	+0.3
3,181,560	+		PP_2791	0.67	+1.3