Strain Fitness in Pseudomonas putida KT2440 around PP_1972

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
2,232,445	+				-0.5
2,232,612	+		PP_1971	0.26	-0.1
2,232,612	+		PP_1971	0.26	-1.1
2,232,613	-		PP_1971	0.26	-0.1
2,232,613	-		PP_1971	0.26	-0.3
2,232,615	+		PP_1971	0.26	+0.1
2,232,615	+		PP_1971	0.26	-0.0
2,232,615	+		PP_1971	0.26	-0.0
2,232,615	+		PP_1971	0.26	+0.1
2,232,616	-		PP_1971	0.27	-0.8
2,232,662	+		PP_1971	0.35	+0.5
2,232,663	-		PP_1971	0.35	-0.6
2,232,663	-		PP_1971	0.35	-1.2
2,232,663	-		PP_1971	0.35	+0.3
2,232,690	+		PP_1971	0.39	-0.6
2,232,690	+		PP_1971	0.39	+0.2
2,232,690	+		PP_1971	0.39	-0.7
2,232,691	-		PP_1971	0.40	-0.6
2,232,702	-		PP_1971	0.41	-1.3
2,232,800	+		PP_1971	0.58	-0.7
2,232,888	+		PP_1971	0.74	-1.5
2,232,888	+		PP_1971	0.74	-1.0
2,232,888	+				+1.5
2,232,888	+		PP_1971	0.74	-0.7
2,232,888	+		PP_1971	0.74	+0.5
2,232,888	+		PP_1971	0.74	-0.6
2,232,888	+		PP_1971	0.74	-0.9
2,232,889	-				+0.5
2,232,889	-		PP_1971	0.74	+0.5
2,232,922	+		PP_1971	0.79	-1.4
2,232,922	+		PP_1971	0.79	-1.2
2,232,922	+		PP_1971	0.79	-0.7
2,232,923	-		PP_1971	0.80	-2.5
2,233,017	+				+0.6
2,233,074	+				-0.3
2,233,137	-				-0.2
2,233,310	+		PP_t63	0.51	+1.1
2,233,356	+				-0.2
2,233,357	-				+1.4
2,233,362	-				+0.3
2,233,410	+				-0.6
2,233,410	+				-0.5
2,233,497	+				-0.7
2,233,497	+				-1.1
2,233,497	+				-1.2
2,233,497	+				-0.9
2,233,497	+				-1.0
2,233,498	-				+1.5
2,233,498	-				-0.3
2,233,498	-				-0.4
2,233,498	-				-1.8
2,233,498	-				-1.1
2,233,752	+		PP_1972	0.29	-0.6
2,233,752	+		PP_1972	0.29	-0.0
2,233,752	+		PP_1972	0.29	+0.3
2,233,753	-		PP_1972	0.29	-0.2
2,233,753	-		PP_1972	0.29	+0.4
2,233,959	+		PP_1972	0.46	-1.8
2,233,960	-		PP_1972	0.46	-1.0
2,234,151	+		PP_1972	0.62	-1.1
2,234,151	+		PP_1972	0.62	-1.8
2,234,152	-		PP_1972	0.62	-1.1
2,234,152	-		PP_1972	0.62	+0.3
2,234,152	-		PP_1972	0.62	-0.6
2,234,152	-		PP_1972	0.62	-1.4
2,234,160	+		PP_1972	0.63	-0.3
2,234,161	-		PP_1972	0.63	+0.5
2,234,215	+		PP_1972	0.67	-1.4
2,234,216	-		PP_1972	0.67	-0.4
2,234,379	+		PP_1972	0.81	-1.1
2,234,380	-		PP_1972	0.81	-0.3
2,234,380	-		PP_1972	0.81	-0.7
2,234,380	-		PP_1972	0.81	-0.3
2,234,406	+		PP_1972	0.83	-0.1
2,234,406	+		PP_1972	0.83	-0.5
2,234,502	-				-1.0
2,234,563	+				-2.8
2,234,564	-				-0.8
2,234,609	+				-1.2
2,234,609	+				-0.7
2,234,634	+				-0.4
2,234,641	+				+0.8
2,234,641	+				-1.5
2,234,642	-				-1.1
2,234,642	-				-1.6
2,234,690	+				-1.3
2,234,690	+				+0.7
2,234,691	-				+0.1
2,234,692	+				+0.0
2,234,693	-				-0.7
2,234,772	-				+0.5
2,234,851	-				+0.6