Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0771 and PP_0772 are separated by 18 nucleotides PP_0772 and PP_0773 are separated by 118 nucleotides PP_0773 and PP_0774 are separated by 79 nucleotides
PP_0771: PP_0771 - mRNA interferase, at 889,708 to 890,055
_0771
PP_0772: PP_0772 - Metallo-beta-lactamase family protein, at 890,074 to 890,718
_0772
PP_0773: PP_0773 - OmpA/MotB domain-containing protein, at 890,837 to 891,547
_0773
PP_0774: PP_0774 - Phosphate acetyltransferase, at 891,627 to 893,714
_0774
Position (kb) 890
891
892 Strain fitness (log2 ratio) -2
-1
0
1
2 at 889.844 kb on + strand, within PP_0771 at 889.845 kb on - strand, within PP_0771 at 889.845 kb on - strand, within PP_0771 at 889.991 kb on + strand, within PP_0771 at 889.992 kb on - strand, within PP_0771 at 889.992 kb on - strand, within PP_0771 at 890.067 kb on - strand at 890.079 kb on + strand at 890.079 kb on + strand at 890.079 kb on + strand at 890.079 kb on + strand at 890.080 kb on - strand at 890.080 kb on - strand at 890.392 kb on + strand, within PP_0772 at 890.392 kb on + strand, within PP_0772 at 890.393 kb on - strand, within PP_0772 at 890.393 kb on - strand, within PP_0772 at 890.393 kb on - strand, within PP_0772 at 890.469 kb on + strand, within PP_0772 at 890.469 kb on + strand, within PP_0772 at 890.470 kb on - strand, within PP_0772 at 890.470 kb on - strand, within PP_0772 at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.778 kb on - strand at 890.778 kb on - strand at 890.778 kb on - strand at 890.778 kb on - strand at 890.778 kb on - strand at 890.816 kb on - strand at 890.903 kb on + strand at 890.904 kb on - strand at 890.957 kb on + strand, within PP_0773 at 891.080 kb on + strand, within PP_0773 at 891.224 kb on + strand, within PP_0773 at 891.365 kb on + strand, within PP_0773 at 891.365 kb on + strand, within PP_0773 at 891.366 kb on - strand, within PP_0773 at 891.366 kb on - strand, within PP_0773 at 891.366 kb on - strand, within PP_0773 at 891.366 kb on - strand, within PP_0773 at 891.405 kb on + strand, within PP_0773 at 891.406 kb on - strand, within PP_0773 at 891.512 kb on + strand at 891.512 kb on + strand at 891.512 kb on + strand at 891.513 kb on - strand at 891.513 kb on - strand at 891.540 kb on - strand at 891.555 kb on + strand at 891.556 kb on - strand at 891.556 kb on - strand at 891.663 kb on + strand at 891.664 kb on - strand at 891.668 kb on + strand at 891.668 kb on + strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.771 kb on + strand at 891.788 kb on + strand at 891.789 kb on - strand at 891.893 kb on - strand, within PP_0774 at 891.997 kb on + strand, within PP_0774 at 891.997 kb on + strand, within PP_0774 at 891.997 kb on + strand, within PP_0774 at 891.998 kb on - strand, within PP_0774 at 892.366 kb on + strand, within PP_0774
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. remove 889,844 + PP_0771 0.39 -2.1 889,845 - PP_0771 0.39 +0.3 889,845 - PP_0771 0.39 -0.6 889,991 + PP_0771 0.81 +0.4 889,992 - PP_0771 0.82 +2.0 889,992 - PP_0771 0.82 -0.8 890,067 - +0.0 890,079 + -1.1 890,079 + -0.9 890,079 + -0.5 890,079 + -0.7 890,080 - -0.4 890,080 - +0.2 890,392 + PP_0772 0.49 -0.2 890,392 + PP_0772 0.49 -1.0 890,393 - PP_0772 0.49 -1.2 890,393 - PP_0772 0.49 +0.6 890,393 - PP_0772 0.49 -0.3 890,469 + PP_0772 0.61 -0.1 890,469 + PP_0772 0.61 -0.8 890,470 - PP_0772 0.61 -0.6 890,470 - PP_0772 0.61 -1.2 890,777 + -0.6 890,777 + -0.1 890,777 + +0.9 890,777 + -1.3 890,777 + +0.2 890,777 + -0.7 890,777 + +0.8 890,777 + +0.7 890,778 - -2.1 890,778 - -1.5 890,778 - -0.5 890,778 - +0.3 890,778 - +0.0 890,816 - -0.6 890,903 + +0.3 890,904 - -0.9 890,957 + PP_0773 0.17 -0.3 891,080 + PP_0773 0.34 +1.1 891,224 + PP_0773 0.54 +0.8 891,365 + PP_0773 0.74 +1.2 891,365 + PP_0773 0.74 +0.5 891,366 - PP_0773 0.74 -0.7 891,366 - PP_0773 0.74 -0.3 891,366 - PP_0773 0.74 -0.8 891,366 - PP_0773 0.74 +0.2 891,405 + PP_0773 0.80 -0.9 891,406 - PP_0773 0.80 +0.1 891,512 + -1.1 891,512 + -0.3 891,512 + +2.4 891,513 - +0.4 891,513 - +2.4 891,540 - -0.8 891,555 + +0.2 891,556 - -0.6 891,556 - -0.4 891,663 + -0.4 891,664 - -0.6 891,668 + -0.1 891,668 + -0.1 891,669 - +1.4 891,669 - -0.6 891,669 - -0.3 891,669 - +1.9 891,669 - +2.4 891,669 - -1.1 891,669 - +0.7 891,669 - +0.7 891,771 + -1.0 891,788 + -1.3 891,789 - -2.0 891,893 - PP_0774 0.13 -1.1 891,997 + PP_0774 0.18 -1.4 891,997 + PP_0774 0.18 -0.4 891,997 + PP_0774 0.18 -0.1 891,998 - PP_0774 0.18 +0.4 892,366 + PP_0774 0.35 +0.3
Or see this region's nucleotide sequence