Strain Fitness in Pseudomonas putida KT2440 around PP_0131

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0130 and PP_0131 are separated by 195 nucleotidesPP_0131 and PP_0132 are separated by 14 nucleotides PP_0130: PP_0130 - 1,6-anhydro-N-acetylmuramyl-L-alanine amidase, at 136,116 to 136,904 _0130 PP_0131: PP_0131 - putative membrane-anchored phosphodiesterase, at 137,100 to 138,707 _0131 PP_0132: PP_0132 - Alginate biosynthesis sensor protein KinB, at 138,722 to 140,503 _0132 Position (kb) 137 138 139Strain fitness (log2 ratio) -2 -1 0 1 2at 136.156 kb on + strandat 136.156 kb on + strandat 136.157 kb on - strandat 136.157 kb on - strandat 136.251 kb on + strand, within PP_0130at 136.251 kb on + strand, within PP_0130at 136.251 kb on + strand, within PP_0130at 136.251 kb on + strand, within PP_0130at 136.252 kb on - strand, within PP_0130at 136.252 kb on - strand, within PP_0130at 136.252 kb on - strand, within PP_0130at 136.252 kb on - strand, within PP_0130at 136.252 kb on - strand, within PP_0130at 136.290 kb on - strand, within PP_0130at 136.314 kb on + strand, within PP_0130at 136.314 kb on + strand, within PP_0130at 136.315 kb on - strand, within PP_0130at 136.315 kb on - strand, within PP_0130at 136.337 kb on + strand, within PP_0130at 136.344 kb on + strand, within PP_0130at 136.344 kb on + strand, within PP_0130at 136.344 kb on + strand, within PP_0130at 136.344 kb on + strand, within PP_0130at 136.345 kb on - strand, within PP_0130at 136.345 kb on - strand, within PP_0130at 136.345 kb on - strand, within PP_0130at 136.354 kb on + strand, within PP_0130at 136.354 kb on + strand, within PP_0130at 136.354 kb on + strand, within PP_0130at 136.355 kb on - strand, within PP_0130at 136.355 kb on - strand, within PP_0130at 136.355 kb on - strandat 136.491 kb on + strand, within PP_0130at 136.491 kb on + strand, within PP_0130at 136.491 kb on + strand, within PP_0130at 136.568 kb on + strand, within PP_0130at 136.659 kb on - strand, within PP_0130at 136.698 kb on + strand, within PP_0130at 136.698 kb on + strand, within PP_0130at 136.699 kb on - strand, within PP_0130at 136.699 kb on - strand, within PP_0130at 136.732 kb on - strand, within PP_0130at 136.850 kb on + strandat 136.851 kb on - strandat 136.885 kb on + strandat 136.885 kb on + strandat 136.886 kb on - strandat 136.974 kb on + strandat 136.983 kb on - strandat 136.983 kb on - strandat 137.014 kb on + strandat 137.014 kb on + strandat 137.064 kb on + strandat 137.079 kb on + strandat 137.079 kb on + strandat 137.081 kb on + strandat 137.082 kb on - strandat 137.082 kb on - strandat 137.090 kb on + strandat 137.412 kb on + strand, within PP_0131at 137.412 kb on + strand, within PP_0131at 137.413 kb on - strand, within PP_0131at 137.424 kb on + strand, within PP_0131at 137.424 kb on + strand, within PP_0131at 137.424 kb on + strand, within PP_0131at 137.425 kb on - strand, within PP_0131at 137.425 kb on - strand, within PP_0131at 137.425 kb on - strand, within PP_0131at 137.425 kb on - strand, within PP_0131at 137.425 kb on - strand, within PP_0131at 137.428 kb on + strand, within PP_0131at 137.428 kb on + strand, within PP_0131at 137.428 kb on + strand, within PP_0131at 137.428 kb on + strand, within PP_0131at 137.428 kb on + strand, within PP_0131at 137.428 kb on + strand, within PP_0131at 137.429 kb on - strand, within PP_0131at 137.429 kb on - strand, within PP_0131at 137.429 kb on - strand, within PP_0131at 137.429 kb on - strand, within PP_0131at 137.429 kb on - strand, within PP_0131at 137.429 kb on - strand, within PP_0131at 137.429 kb on - strand, within PP_0131at 137.495 kb on + strand, within PP_0131at 137.511 kb on + strand, within PP_0131at 137.511 kb on + strand, within PP_0131at 137.512 kb on - strand, within PP_0131at 137.512 kb on - strand, within PP_0131at 137.512 kb on - strand, within PP_0131at 137.512 kb on - strand, within PP_0131at 137.512 kb on - strand, within PP_0131at 137.517 kb on + strand, within PP_0131at 137.517 kb on + strand, within PP_0131at 137.517 kb on + strand, within PP_0131at 137.517 kb on + strand, within PP_0131at 137.526 kb on - strand, within PP_0131at 137.526 kb on - strand, within PP_0131at 137.526 kb on - strand, within PP_0131at 137.704 kb on + strand, within PP_0131at 137.704 kb on + strand, within PP_0131at 137.704 kb on + strand, within PP_0131at 137.704 kb on + strand, within PP_0131at 137.704 kb on + strand, within PP_0131at 137.704 kb on + strand, within PP_0131at 137.705 kb on - strand, within PP_0131at 137.705 kb on - strand, within PP_0131at 137.799 kb on + strand, within PP_0131at 137.912 kb on - strand, within PP_0131at 137.992 kb on - strand, within PP_0131at 138.175 kb on + strand, within PP_0131at 138.175 kb on + strand, within PP_0131at 138.176 kb on - strand, within PP_0131at 138.176 kb on - strand, within PP_0131at 138.176 kb on - strand, within PP_0131at 138.196 kb on + strand, within PP_0131at 138.196 kb on + strand, within PP_0131at 138.196 kb on + strand, within PP_0131at 138.197 kb on - strand, within PP_0131at 138.197 kb on - strand, within PP_0131at 138.197 kb on - strand, within PP_0131at 138.198 kb on + strand, within PP_0131at 138.198 kb on + strand, within PP_0131at 138.198 kb on + strand, within PP_0131at 138.198 kb on + strand, within PP_0131at 138.198 kb on + strand, within PP_0131at 138.198 kb on + strand, within PP_0131at 138.198 kb on + strand, within PP_0131at 138.198 kb on + strand, within PP_0131at 138.198 kb on + strand, within PP_0131at 138.198 kb on + strand, within PP_0131at 138.198 kb on + strand, within PP_0131at 138.198 kb on + strand, within PP_0131at 138.199 kb on - strand, within PP_0131at 138.199 kb on - strand, within PP_0131at 138.199 kb on - strand, within PP_0131at 138.199 kb on - strand, within PP_0131at 138.199 kb on - strand, within PP_0131at 138.199 kb on - strand, within PP_0131at 138.199 kb on - strand, within PP_0131at 138.199 kb on - strand, within PP_0131at 138.199 kb on - strand, within PP_0131at 138.199 kb on - strand, within PP_0131at 138.199 kb on - strand, within PP_0131at 138.202 kb on + strand, within PP_0131at 138.202 kb on + strand, within PP_0131at 138.203 kb on - strand, within PP_0131at 138.243 kb on - strand, within PP_0131at 138.243 kb on - strand, within PP_0131at 138.243 kb on - strand, within PP_0131at 138.243 kb on - strand, within PP_0131at 138.243 kb on - strand, within PP_0131at 138.243 kb on - strand, within PP_0131at 138.365 kb on - strand, within PP_0131at 138.365 kb on - strand, within PP_0131at 138.365 kb on - strandat 138.411 kb on + strand, within PP_0131at 138.412 kb on - strand, within PP_0131at 138.426 kb on + strand, within PP_0131at 138.521 kb on + strand, within PP_0131at 138.522 kb on - strand, within PP_0131at 138.522 kb on - strand, within PP_0131at 138.618 kb on + strandat 138.618 kb on + strandat 138.618 kb on + strandat 138.619 kb on - strandat 138.619 kb on - strandat 138.706 kb on - strandat 138.741 kb on + strandat 138.742 kb on - strandat 138.855 kb on - strandat 138.855 kb on - strandat 138.894 kb on + strandat 138.985 kb on + strand, within PP_0132at 139.228 kb on + strand, within PP_0132at 139.229 kb on - strand, within PP_0132at 139.479 kb on + strand, within PP_0132at 139.480 kb on - strand, within PP_0132at 139.480 kb on - strand, within PP_0132at 139.480 kb on - strand, within PP_0132at 139.480 kb on - strand, within PP_0132

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Per-strain Table

Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
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136,156 + -0.5
136,156 + +0.1
136,157 - -0.3
136,157 - +0.7
136,251 + PP_0130 0.17 +0.0
136,251 + PP_0130 0.17 +0.1
136,251 + PP_0130 0.17 +0.6
136,251 + PP_0130 0.17 -0.6
136,252 - PP_0130 0.17 +2.3
136,252 - PP_0130 0.17 -0.8
136,252 - PP_0130 0.17 +0.7
136,252 - PP_0130 0.17 +0.2
136,252 - PP_0130 0.17 -0.3
136,290 - PP_0130 0.22 -1.1
136,314 + PP_0130 0.25 +0.4
136,314 + PP_0130 0.25 +0.4
136,315 - PP_0130 0.25 -1.5
136,315 - PP_0130 0.25 +0.0
136,337 + PP_0130 0.28 -0.8
136,344 + PP_0130 0.29 +0.5
136,344 + PP_0130 0.29 +0.5
136,344 + PP_0130 0.29 +1.7
136,344 + PP_0130 0.29 -0.2
136,345 - PP_0130 0.29 +0.0
136,345 - PP_0130 0.29 +0.2
136,345 - PP_0130 0.29 +0.2
136,354 + PP_0130 0.30 +0.3
136,354 + PP_0130 0.30 +0.3
136,354 + PP_0130 0.30 +1.3
136,355 - PP_0130 0.30 -0.6
136,355 - PP_0130 0.30 +2.3
136,355 - +1.3
136,491 + PP_0130 0.48 +0.8
136,491 + PP_0130 0.48 +0.1
136,491 + PP_0130 0.48 +0.2
136,568 + PP_0130 0.57 -0.1
136,659 - PP_0130 0.69 +0.4
136,698 + PP_0130 0.74 -1.2
136,698 + PP_0130 0.74 -1.2
136,699 - PP_0130 0.74 +0.1
136,699 - PP_0130 0.74 -0.9
136,732 - PP_0130 0.78 +0.9
136,850 + -1.3
136,851 - +0.2
136,885 + +2.3
136,885 + +0.0
136,886 - -0.3
136,974 + -0.1
136,983 - -0.6
136,983 - +2.3
137,014 + +0.6
137,014 + +0.1
137,064 + +0.2
137,079 + -0.4
137,079 + -0.1
137,081 + -0.9
137,082 - +2.3
137,082 - -1.0
137,090 + +0.2
137,412 + PP_0131 0.19 -0.6
137,412 + PP_0131 0.19 -0.5
137,413 - PP_0131 0.19 -0.1
137,424 + PP_0131 0.20 +1.3
137,424 + PP_0131 0.20 -0.2
137,424 + PP_0131 0.20 -1.3
137,425 - PP_0131 0.20 -0.1
137,425 - PP_0131 0.20 +2.3
137,425 - PP_0131 0.20 -1.0
137,425 - PP_0131 0.20 +1.0
137,425 - PP_0131 0.20 +0.0
137,428 + PP_0131 0.20 -2.3
137,428 + PP_0131 0.20 -0.7
137,428 + PP_0131 0.20 -0.1
137,428 + PP_0131 0.20 -0.8
137,428 + PP_0131 0.20 +0.5
137,428 + PP_0131 0.20 -1.1
137,429 - PP_0131 0.20 +0.4
137,429 - PP_0131 0.20 +0.7
137,429 - PP_0131 0.20 +1.0
137,429 - PP_0131 0.20 +0.7
137,429 - PP_0131 0.20 +1.0
137,429 - PP_0131 0.20 -0.9
137,429 - PP_0131 0.20 -0.6
137,495 + PP_0131 0.25 -1.4
137,511 + PP_0131 0.26 -1.8
137,511 + PP_0131 0.26 +0.3
137,512 - PP_0131 0.26 -0.9
137,512 - PP_0131 0.26 -0.2
137,512 - PP_0131 0.26 -0.5
137,512 - PP_0131 0.26 +0.1
137,512 - PP_0131 0.26 -1.1
137,517 + PP_0131 0.26 -0.0
137,517 + PP_0131 0.26 +0.3
137,517 + PP_0131 0.26 +0.2
137,517 + PP_0131 0.26 +1.3
137,526 - PP_0131 0.26 -0.2
137,526 - PP_0131 0.26 -0.5
137,526 - PP_0131 0.26 -1.2
137,704 + PP_0131 0.38 +0.6
137,704 + PP_0131 0.38 +0.2
137,704 + PP_0131 0.38 +0.5
137,704 + PP_0131 0.38 +2.3
137,704 + PP_0131 0.38 -0.1
137,704 + PP_0131 0.38 -0.7
137,705 - PP_0131 0.38 -0.5
137,705 - PP_0131 0.38 -0.5
137,799 + PP_0131 0.43 -0.5
137,912 - PP_0131 0.50 -0.6
137,992 - PP_0131 0.55 -0.8
138,175 + PP_0131 0.67 +0.2
138,175 + PP_0131 0.67 -1.1
138,176 - PP_0131 0.67 +0.7
138,176 - PP_0131 0.67 -0.5
138,176 - PP_0131 0.67 +0.1
138,196 + PP_0131 0.68 -0.8
138,196 + PP_0131 0.68 -0.1
138,196 + PP_0131 0.68 +0.8
138,197 - PP_0131 0.68 +0.3
138,197 - PP_0131 0.68 -1.0
138,197 - PP_0131 0.68 +0.9
138,198 + PP_0131 0.68 -0.2
138,198 + PP_0131 0.68 +0.2
138,198 + PP_0131 0.68 +0.7
138,198 + PP_0131 0.68 -1.8
138,198 + PP_0131 0.68 -0.8
138,198 + PP_0131 0.68 -0.4
138,198 + PP_0131 0.68 -0.3
138,198 + PP_0131 0.68 -0.4
138,198 + PP_0131 0.68 -0.1
138,198 + PP_0131 0.68 +0.2
138,198 + PP_0131 0.68 +0.2
138,198 + PP_0131 0.68 +0.6
138,199 - PP_0131 0.68 -0.6
138,199 - PP_0131 0.68 -1.9
138,199 - PP_0131 0.68 +1.0
138,199 - PP_0131 0.68 +2.3
138,199 - PP_0131 0.68 -2.1
138,199 - PP_0131 0.68 -1.5
138,199 - PP_0131 0.68 +0.3
138,199 - PP_0131 0.68 -0.3
138,199 - PP_0131 0.68 +0.4
138,199 - PP_0131 0.68 -0.9
138,199 - PP_0131 0.68 -1.3
138,202 + PP_0131 0.69 -0.2
138,202 + PP_0131 0.69 -0.6
138,203 - PP_0131 0.69 -0.2
138,243 - PP_0131 0.71 +0.3
138,243 - PP_0131 0.71 +0.1
138,243 - PP_0131 0.71 -0.3
138,243 - PP_0131 0.71 +0.2
138,243 - PP_0131 0.71 -0.7
138,243 - PP_0131 0.71 +0.3
138,365 - PP_0131 0.79 -1.1
138,365 - PP_0131 0.79 +0.8
138,365 - +0.0
138,411 + PP_0131 0.82 +0.2
138,412 - PP_0131 0.82 +0.4
138,426 + PP_0131 0.82 -0.1
138,521 + PP_0131 0.88 -0.9
138,522 - PP_0131 0.88 -0.5
138,522 - PP_0131 0.88 -0.5
138,618 + -0.7
138,618 + +0.6
138,618 + -0.2
138,619 - -1.1
138,619 - -0.1
138,706 - -0.9
138,741 + +1.1
138,742 - +1.0
138,855 - -0.3
138,855 - -1.3
138,894 + -0.7
138,985 + PP_0132 0.15 -1.8
139,228 + PP_0132 0.28 -0.5
139,229 - PP_0132 0.28 -0.1
139,479 + PP_0132 0.42 +0.6
139,480 - PP_0132 0.43 -0.2
139,480 - PP_0132 0.43 +0.9
139,480 - PP_0132 0.43 +2.3
139,480 - PP_0132 0.43 +0.1

Or see this region's nucleotide sequence