Strain Fitness in Escherichia coli BW25113 around b2740

Experiment: LB Aerobic with Tetracycline 0.0000003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygbL and ygbM are separated by 4 nucleotidesygbM and ygbN are separated by 88 nucleotidesygbN and rpoS are separated by 93 nucleotides b2738: ygbL - hypothetical protein (NCBI), at 2,861,615 to 2,862,253 ygbL b2739: ygbM - hypothetical protein (NCBI), at 2,862,258 to 2,863,034 ygbM b2740: ygbN - predicted transporter (NCBI), at 2,863,123 to 2,864,487 ygbN b2741: rpoS - RNA polymerase sigma factor (NCBI), at 2,864,581 to 2,865,573 rpoS Position (kb) 2863 2864 2865Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2862.384 kb on - strand, within ygbMat 2862.439 kb on - strand, within ygbMat 2862.439 kb on - strand, within ygbMat 2862.479 kb on - strand, within ygbMat 2862.511 kb on - strand, within ygbMat 2862.531 kb on + strand, within ygbMat 2862.569 kb on - strand, within ygbMat 2862.569 kb on - strand, within ygbMat 2862.598 kb on + strand, within ygbMat 2862.608 kb on + strand, within ygbMat 2862.660 kb on - strand, within ygbMat 2862.695 kb on - strand, within ygbMat 2862.708 kb on - strand, within ygbMat 2862.741 kb on - strand, within ygbMat 2862.743 kb on + strand, within ygbMat 2862.743 kb on + strand, within ygbMat 2862.753 kb on - strand, within ygbMat 2862.756 kb on - strand, within ygbMat 2862.767 kb on + strand, within ygbMat 2862.775 kb on - strand, within ygbMat 2862.775 kb on - strand, within ygbMat 2862.898 kb on - strand, within ygbMat 2862.903 kb on - strand, within ygbMat 2862.903 kb on - strand, within ygbMat 2862.936 kb on - strand, within ygbMat 2862.947 kb on + strand, within ygbMat 2862.947 kb on + strand, within ygbMat 2862.967 kb on - strandat 2862.997 kb on + strandat 2863.069 kb on - strandat 2863.084 kb on - strandat 2863.114 kb on + strandat 2863.140 kb on + strandat 2863.261 kb on - strand, within ygbNat 2863.261 kb on - strand, within ygbNat 2863.338 kb on - strand, within ygbNat 2863.342 kb on + strand, within ygbNat 2863.342 kb on + strand, within ygbNat 2863.342 kb on + strand, within ygbNat 2863.370 kb on + strand, within ygbNat 2863.370 kb on + strand, within ygbNat 2863.435 kb on - strand, within ygbNat 2863.571 kb on - strand, within ygbNat 2863.582 kb on + strand, within ygbNat 2863.627 kb on + strand, within ygbNat 2863.627 kb on + strand, within ygbNat 2863.627 kb on + strand, within ygbNat 2863.627 kb on + strand, within ygbNat 2863.693 kb on - strand, within ygbNat 2863.751 kb on + strand, within ygbNat 2863.782 kb on - strand, within ygbNat 2863.832 kb on + strand, within ygbNat 2863.861 kb on - strand, within ygbNat 2863.861 kb on - strand, within ygbNat 2863.933 kb on + strand, within ygbNat 2863.961 kb on - strand, within ygbNat 2863.961 kb on - strand, within ygbNat 2864.020 kb on + strand, within ygbNat 2864.020 kb on + strand, within ygbNat 2864.026 kb on - strand, within ygbNat 2864.036 kb on + strand, within ygbNat 2864.038 kb on + strand, within ygbNat 2864.038 kb on + strand, within ygbNat 2864.101 kb on - strand, within ygbNat 2864.148 kb on - strand, within ygbNat 2864.150 kb on + strand, within ygbNat 2864.231 kb on - strand, within ygbNat 2864.231 kb on - strand, within ygbNat 2864.395 kb on + strandat 2864.422 kb on + strandat 2864.463 kb on - strandat 2864.590 kb on + strandat 2864.608 kb on - strandat 2864.608 kb on - strandat 2864.641 kb on + strandat 2864.656 kb on - strandat 2864.698 kb on + strand, within rpoSat 2864.840 kb on - strandat 2864.866 kb on + strand, within rpoSat 2864.925 kb on + strand, within rpoSat 2864.925 kb on + strand, within rpoSat 2865.021 kb on + strand, within rpoSat 2865.037 kb on + strand, within rpoSat 2865.044 kb on + strand, within rpoSat 2865.047 kb on - strand, within rpoSat 2865.085 kb on - strand, within rpoSat 2865.116 kb on - strand, within rpoSat 2865.175 kb on + strand, within rpoSat 2865.190 kb on + strand, within rpoSat 2865.195 kb on + strand, within rpoSat 2865.198 kb on - strand, within rpoSat 2865.376 kb on - strand, within rpoSat 2865.389 kb on + strand, within rpoSat 2865.415 kb on + strand, within rpoSat 2865.447 kb on - strand, within rpoS

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Tetracycline 0.0000003125 mM
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2,862,384 - ygbM b2739 0.16 +0.1
2,862,439 - ygbM b2739 0.23 -0.2
2,862,439 - ygbM b2739 0.23 +0.8
2,862,479 - ygbM b2739 0.28 +0.6
2,862,511 - ygbM b2739 0.33 -0.0
2,862,531 + ygbM b2739 0.35 +1.2
2,862,569 - ygbM b2739 0.40 -1.8
2,862,569 - ygbM b2739 0.40 +0.1
2,862,598 + ygbM b2739 0.44 -0.8
2,862,608 + ygbM b2739 0.45 +0.8
2,862,660 - ygbM b2739 0.52 +1.1
2,862,695 - ygbM b2739 0.56 +1.1
2,862,708 - ygbM b2739 0.58 +0.8
2,862,741 - ygbM b2739 0.62 +1.4
2,862,743 + ygbM b2739 0.62 +0.4
2,862,743 + ygbM b2739 0.62 +1.4
2,862,753 - ygbM b2739 0.64 +0.8
2,862,756 - ygbM b2739 0.64 +0.2
2,862,767 + ygbM b2739 0.66 -0.8
2,862,775 - ygbM b2739 0.67 -0.8
2,862,775 - ygbM b2739 0.67 +1.4
2,862,898 - ygbM b2739 0.82 +0.3
2,862,903 - ygbM b2739 0.83 +0.6
2,862,903 - ygbM b2739 0.83 -3.3
2,862,936 - ygbM b2739 0.87 +0.7
2,862,947 + ygbM b2739 0.89 +0.5
2,862,947 + ygbM b2739 0.89 +0.3
2,862,967 - -0.0
2,862,997 + -1.0
2,863,069 - -0.3
2,863,084 - +0.1
2,863,114 + -0.5
2,863,140 + +0.0
2,863,261 - ygbN b2740 0.10 -2.0
2,863,261 - ygbN b2740 0.10 -1.2
2,863,338 - ygbN b2740 0.16 -1.5
2,863,342 + ygbN b2740 0.16 -0.4
2,863,342 + ygbN b2740 0.16 +0.3
2,863,342 + ygbN b2740 0.16 +0.2
2,863,370 + ygbN b2740 0.18 -0.2
2,863,370 + ygbN b2740 0.18 +1.1
2,863,435 - ygbN b2740 0.23 +1.8
2,863,571 - ygbN b2740 0.33 +1.1
2,863,582 + ygbN b2740 0.34 +0.8
2,863,627 + ygbN b2740 0.37 +0.7
2,863,627 + ygbN b2740 0.37 -0.0
2,863,627 + ygbN b2740 0.37 -0.8
2,863,627 + ygbN b2740 0.37 +0.1
2,863,693 - ygbN b2740 0.42 -0.6
2,863,751 + ygbN b2740 0.46 -0.2
2,863,782 - ygbN b2740 0.48 -0.0
2,863,832 + ygbN b2740 0.52 +0.3
2,863,861 - ygbN b2740 0.54 +0.8
2,863,861 - ygbN b2740 0.54 -1.2
2,863,933 + ygbN b2740 0.59 -0.6
2,863,961 - ygbN b2740 0.61 +0.8
2,863,961 - ygbN b2740 0.61 -0.2
2,864,020 + ygbN b2740 0.66 -0.6
2,864,020 + ygbN b2740 0.66 +0.4
2,864,026 - ygbN b2740 0.66 -0.2
2,864,036 + ygbN b2740 0.67 +0.2
2,864,038 + ygbN b2740 0.67 +0.3
2,864,038 + ygbN b2740 0.67 -0.2
2,864,101 - ygbN b2740 0.72 -2.4
2,864,148 - ygbN b2740 0.75 +0.4
2,864,150 + ygbN b2740 0.75 -0.0
2,864,231 - ygbN b2740 0.81 -1.8
2,864,231 - ygbN b2740 0.81 +0.4
2,864,395 + -1.1
2,864,422 + -0.8
2,864,463 - -1.0
2,864,590 + -2.0
2,864,608 - +0.9
2,864,608 - +0.0
2,864,641 + +0.8
2,864,656 - +0.4
2,864,698 + rpoS b2741 0.12 -0.5
2,864,840 - +0.8
2,864,866 + rpoS b2741 0.29 +1.8
2,864,925 + rpoS b2741 0.35 -0.4
2,864,925 + rpoS b2741 0.35 -3.2
2,865,021 + rpoS b2741 0.44 +0.8
2,865,037 + rpoS b2741 0.46 +0.2
2,865,044 + rpoS b2741 0.47 +0.5
2,865,047 - rpoS b2741 0.47 +0.7
2,865,085 - rpoS b2741 0.51 +0.7
2,865,116 - rpoS b2741 0.54 +0.4
2,865,175 + rpoS b2741 0.60 +0.8
2,865,190 + rpoS b2741 0.61 -0.8
2,865,195 + rpoS b2741 0.62 +1.8
2,865,198 - rpoS b2741 0.62 +0.2
2,865,376 - rpoS b2741 0.80 -0.5
2,865,389 + rpoS b2741 0.81 -0.9
2,865,415 + rpoS b2741 0.84 +0.1
2,865,447 - rpoS b2741 0.87 +0.6

Or see this region's nucleotide sequence