Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5448 and PP_5449 are separated by 65 nucleotides PP_5449 and PP_0639 are separated by 199 nucleotides PP_0639 and PP_0640 overlap by 4 nucleotides
PP_5448: PP_5448 - conserved protein of unknown function, at 747,667 to 747,882
_5448
PP_5449: PP_5449 - conserved exported protein of unknown function, at 747,948 to 748,766
_5449
PP_0639: PP_0639 - conserved protein of unknown function, at 748,966 to 749,682
_0639
PP_0640: PP_0640 - conserved protein of unknown function, at 749,679 to 750,863
_0640
Position (kb) 747
748
749 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 746.965 kb on + strand at 746.965 kb on + strand at 746.984 kb on + strand at 746.984 kb on + strand at 746.985 kb on - strand at 746.985 kb on - strand at 746.985 kb on - strand at 746.985 kb on - strand at 746.985 kb on - strand at 746.985 kb on - strand at 746.985 kb on - strand at 747.037 kb on + strand at 747.037 kb on + strand at 747.038 kb on - strand at 747.063 kb on + strand at 747.064 kb on - strand at 747.064 kb on - strand at 747.168 kb on + strand at 747.230 kb on - strand at 747.259 kb on + strand at 747.259 kb on + strand at 747.259 kb on + strand at 747.268 kb on - strand at 747.305 kb on - strand at 747.305 kb on - strand at 747.367 kb on + strand at 747.368 kb on - strand at 747.405 kb on + strand at 747.405 kb on + strand at 747.405 kb on + strand at 747.406 kb on - strand at 747.497 kb on + strand at 747.498 kb on - strand at 747.498 kb on - strand at 747.498 kb on - strand at 747.498 kb on - strand at 747.511 kb on + strand at 747.511 kb on + strand at 747.512 kb on - strand at 747.683 kb on + strand at 747.720 kb on + strand, within PP_5448 at 747.720 kb on + strand, within PP_5448 at 747.721 kb on - strand, within PP_5448 at 747.787 kb on + strand, within PP_5448 at 747.792 kb on + strand, within PP_5448 at 747.792 kb on + strand, within PP_5448 at 747.886 kb on + strand at 747.954 kb on - strand at 747.977 kb on - strand at 748.034 kb on + strand, within PP_5449 at 748.034 kb on + strand, within PP_5449 at 748.035 kb on - strand, within PP_5449 at 748.035 kb on - strand, within PP_5449 at 748.039 kb on - strand, within PP_5449 at 748.039 kb on - strand, within PP_5449 at 748.110 kb on - strand, within PP_5449 at 748.127 kb on + strand, within PP_5449 at 748.253 kb on + strand, within PP_5449 at 748.312 kb on - strand, within PP_5449 at 748.328 kb on - strand, within PP_5449 at 748.494 kb on - strand, within PP_5449 at 748.566 kb on + strand, within PP_5449 at 748.738 kb on - strand at 748.773 kb on - strand at 748.812 kb on + strand at 748.812 kb on + strand at 748.879 kb on + strand at 748.880 kb on - strand at 749.101 kb on - strand, within PP_0639 at 749.141 kb on + strand, within PP_0639 at 749.239 kb on - strand, within PP_0639 at 749.279 kb on - strand, within PP_0639 at 749.308 kb on + strand, within PP_0639 at 749.309 kb on - strand, within PP_0639 at 749.309 kb on - strand, within PP_0639 at 749.315 kb on - strand, within PP_0639 at 749.355 kb on + strand, within PP_0639 at 749.421 kb on - strand, within PP_0639 at 749.652 kb on - strand at 749.652 kb on - strand at 749.704 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 746,965 + +0.9 746,965 + -0.8 746,984 + -0.9 746,984 + -0.9 746,985 - +0.5 746,985 - -1.3 746,985 - -0.3 746,985 - -0.4 746,985 - +1.0 746,985 - -1.1 746,985 - -0.9 747,037 + -0.9 747,037 + +0.4 747,038 - +1.1 747,063 + +0.1 747,064 - -0.6 747,064 - -0.5 747,168 + -0.7 747,230 - +0.0 747,259 + +0.7 747,259 + +0.3 747,259 + +0.4 747,268 - -0.7 747,305 - -2.1 747,305 - +0.1 747,367 + -0.4 747,368 - -0.4 747,405 + -0.2 747,405 + -1.1 747,405 + -0.2 747,406 - -0.1 747,497 + +0.5 747,498 - +0.3 747,498 - -0.1 747,498 - +1.4 747,498 - +0.2 747,511 + +2.6 747,511 + +0.4 747,512 - -1.6 747,683 + +0.0 747,720 + PP_5448 0.25 -1.4 747,720 + PP_5448 0.25 +0.8 747,721 - PP_5448 0.25 -0.1 747,787 + PP_5448 0.56 -0.4 747,792 + PP_5448 0.58 +3.2 747,792 + PP_5448 0.58 +0.7 747,886 + +0.5 747,954 - -0.4 747,977 - +0.5 748,034 + PP_5449 0.11 +0.5 748,034 + PP_5449 0.11 -0.6 748,035 - PP_5449 0.11 -0.9 748,035 - PP_5449 0.11 -1.4 748,039 - PP_5449 0.11 -0.2 748,039 - PP_5449 0.11 -0.5 748,110 - PP_5449 0.20 -0.5 748,127 + PP_5449 0.22 -0.7 748,253 + PP_5449 0.37 -0.3 748,312 - PP_5449 0.44 -1.6 748,328 - PP_5449 0.46 +2.6 748,494 - PP_5449 0.67 -1.3 748,566 + PP_5449 0.75 -1.0 748,738 - +0.4 748,773 - +0.0 748,812 + +0.2 748,812 + +0.0 748,879 + -1.7 748,880 - +1.6 749,101 - PP_0639 0.19 -0.8 749,141 + PP_0639 0.24 -1.1 749,239 - PP_0639 0.38 -2.4 749,279 - PP_0639 0.44 +0.4 749,308 + PP_0639 0.48 -1.7 749,309 - PP_0639 0.48 +1.3 749,309 - PP_0639 0.48 -0.2 749,315 - PP_0639 0.49 +0.6 749,355 + PP_0639 0.54 -0.9 749,421 - PP_0639 0.63 -0.2 749,652 - +0.7 749,652 - -0.0 749,704 + -0.4
Or see this region's nucleotide sequence