Strain Fitness in Pseudomonas putida KT2440 around PP_5126

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
5,845,903	-				+1.5
5,845,986	-				+0.4
5,845,986	-				+1.5
5,846,041	+				-0.5
5,846,042	-				-0.7
5,846,091	+				-0.3
5,846,091	+				-0.1
5,846,092	-				-0.8
5,846,143	+		PP_5125	0.15	-0.2
5,846,245	+		PP_5125	0.28	-0.3
5,846,317	+		PP_5125	0.37	-2.6
5,846,318	-		PP_5125	0.37	-0.8
5,846,454	+		PP_5125	0.54	-0.6
5,846,455	-		PP_5125	0.54	+0.5
5,846,455	-		PP_5125	0.54	-0.6
5,846,455	-		PP_5125	0.54	-0.9
5,846,516	-		PP_5125	0.61	-0.2
5,846,631	-		PP_5125	0.75	-0.1
5,846,656	+		PP_5125	0.78	+0.8
5,846,656	+		PP_5125	0.78	-1.1
5,846,656	+		PP_5125	0.78	+0.4
5,846,657	-		PP_5125	0.78	+0.4
5,846,793	-				-0.0
5,846,811	+				-0.7
5,846,812	-				-0.3
5,846,902	+				-0.5
5,846,902	+				+0.1
5,846,903	-				+0.4
5,846,981	+				+0.9
5,846,981	+		PP_5126	0.17	-0.0
5,846,981	+		PP_5126	0.17	-0.5
5,846,982	-		PP_5126	0.17	-0.4
5,847,044	+		PP_5126	0.24	-0.2
5,847,045	-		PP_5126	0.24	+0.8
5,847,133	+		PP_5126	0.34	+0.7
5,847,134	-		PP_5126	0.34	+0.2
5,847,134	-		PP_5126	0.34	+0.9
5,847,211	+		PP_5126	0.43	-0.0
5,847,212	-		PP_5126	0.43	-1.1
5,847,212	-		PP_5126	0.43	-2.6
5,847,212	-		PP_5126	0.43	-1.8
5,847,212	-		PP_5126	0.43	-2.3
5,847,212	-		PP_5126	0.43	+0.5
5,847,212	-		PP_5126	0.43	+0.3
5,847,235	+		PP_5126	0.46	-0.1
5,847,236	-		PP_5126	0.46	-0.6
5,847,293	+		PP_5126	0.52	-0.8
5,847,293	+		PP_5126	0.52	-1.3
5,847,293	+		PP_5126	0.52	-0.5
5,847,293	+		PP_5126	0.52	-1.6
5,847,294	-		PP_5126	0.53	-1.2
5,847,295	+		PP_5126	0.53	+1.4
5,847,295	+		PP_5126	0.53	+0.9
5,847,295	+		PP_5126	0.53	-0.7
5,847,296	-		PP_5126	0.53	-2.3
5,847,296	-		PP_5126	0.53	+1.3
5,847,296	-		PP_5126	0.53	-0.5
5,847,316	-		PP_5126	0.55	+1.0
5,847,323	+		PP_5126	0.56	-0.5
5,847,458	+		PP_5126	0.71	-0.2
5,847,459	-		PP_5126	0.71	+0.8
5,847,462	+		PP_5126	0.72	+0.1
5,847,463	-		PP_5126	0.72	-0.8
5,847,463	-		PP_5126	0.72	-1.7
5,847,463	-		PP_5126	0.72	-0.7
5,847,463	-		PP_5126	0.72	-1.2
5,847,463	-		PP_5126	0.72	-0.3
5,847,607	+		PP_5126	0.88	-0.6
5,847,607	+		PP_5126	0.88	+1.9
5,847,608	-		PP_5126	0.89	-0.9
5,847,608	-		PP_5126	0.89	-0.5
5,847,608	-		PP_5126	0.89	+0.5
5,847,608	-		PP_5126	0.89	-0.9
5,847,608	-		PP_5126	0.89	-1.7
5,847,608	-		PP_5126	0.89	-0.0
5,847,707	+				-0.1
5,847,707	+				-0.5
5,847,707	+				-0.9
5,847,707	+				+0.5
5,847,708	-				-0.8
5,847,708	-				+0.0
5,847,708	-				-0.1
5,847,708	-				+0.3
5,847,708	-				+0.1
5,847,739	-				-0.8
5,847,746	+				-0.7
5,847,747	-				-0.7
5,847,771	+				-0.8
5,847,772	-				+0.8
5,847,797	+				-0.1
5,847,797	+				-0.8
5,847,797	+				+1.2
5,847,797	+				+0.3
5,847,798	-				+0.7
5,847,798	-				+0.3
5,847,798	-				+0.4
5,847,799	+				+0.2
5,847,799	+				+0.2
5,847,800	-				-0.5
5,847,899	-				-1.5
5,847,899	-				-0.9
5,847,899	-				+0.9
5,848,080	-		PP_5127	0.21	+0.1
5,848,080	-		PP_5127	0.21	+1.3
5,848,136	-		PP_5127	0.26	-0.3
5,848,147	+		PP_5127	0.27	+0.1
5,848,339	+		PP_5127	0.43	-0.7
5,848,340	-		PP_5127	0.43	+0.2
5,848,354	+		PP_5127	0.44	-0.8
5,848,354	+		PP_5127	0.44	-1.3
5,848,445	-		PP_5127	0.51	+1.0
5,848,445	-		PP_5127	0.51	+2.5
5,848,445	-		PP_5127	0.51	-1.1
5,848,480	+		PP_5127	0.54	+1.9
5,848,499	+		PP_5127	0.56	+1.1
5,848,499	+		PP_5127	0.56	+0.5
5,848,517	-		PP_5127	0.57	-1.4