Strain Fitness in Pseudomonas putida KT2440 around PP_4871

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntPP_4869 and PP_4870 are separated by 102 nucleotidesPP_4870 and PP_4871 are separated by 347 nucleotidesPP_4871 and PP_4872 are separated by 120 nucleotides PP_4869: PP_4869 - NH(3)-dependent NAD(+) synthetase, at 5,536,196 to 5,537,023 _4869 PP_4870: PP_4870 - Azurin, at 5,537,126 to 5,537,575 _4870 PP_4871: PP_4871 - putative lysine decarboxylase family protein, at 5,537,923 to 5,538,510 _4871 PP_4872: PP_4872 - putative enzyme of unknown function, at 5,538,631 to 5,540,985 _4872 Position (kb) 5537 5538 5539Strain fitness (log2 ratio) -2 -1 0 1 2 3at 5537.024 kb on + strandat 5537.024 kb on + strandat 5537.025 kb on - strandat 5537.025 kb on - strandat 5537.025 kb on - strandat 5537.025 kb on - strandat 5537.025 kb on - strandat 5537.057 kb on + strandat 5537.127 kb on + strandat 5537.127 kb on + strandat 5537.191 kb on - strand, within PP_4870at 5537.191 kb on - strand, within PP_4870at 5537.301 kb on + strand, within PP_4870at 5537.302 kb on - strand, within PP_4870at 5537.472 kb on + strand, within PP_4870at 5537.473 kb on - strand, within PP_4870at 5537.558 kb on + strandat 5537.599 kb on + strandat 5537.599 kb on + strandat 5537.642 kb on + strandat 5537.643 kb on - strandat 5537.697 kb on + strandat 5537.697 kb on + strandat 5537.765 kb on + strandat 5537.765 kb on + strandat 5537.795 kb on - strandat 5537.931 kb on - strandat 5537.981 kb on - strandat 5538.040 kb on + strand, within PP_4871at 5538.041 kb on - strand, within PP_4871at 5538.041 kb on - strand, within PP_4871at 5538.256 kb on - strand, within PP_4871at 5538.343 kb on + strand, within PP_4871at 5538.372 kb on + strand, within PP_4871at 5538.372 kb on + strand, within PP_4871at 5538.483 kb on + strandat 5538.484 kb on - strandat 5538.620 kb on + strandat 5538.621 kb on - strandat 5538.621 kb on - strandat 5538.632 kb on + strandat 5538.878 kb on + strand, within PP_4872at 5538.878 kb on + strand, within PP_4872at 5538.879 kb on - strand, within PP_4872at 5538.879 kb on - strand, within PP_4872at 5538.879 kb on - strand, within PP_4872at 5538.882 kb on + strand, within PP_4872at 5538.883 kb on - strand, within PP_4872at 5538.883 kb on - strand, within PP_4872at 5538.883 kb on - strand, within PP_4872at 5538.883 kb on - strand, within PP_4872at 5538.883 kb on - strand, within PP_4872at 5539.067 kb on + strand, within PP_4872at 5539.068 kb on - strand, within PP_4872at 5539.103 kb on + strand, within PP_4872at 5539.103 kb on + strand, within PP_4872at 5539.103 kb on + strand, within PP_4872at 5539.103 kb on + strand, within PP_4872at 5539.103 kb on + strand, within PP_4872at 5539.104 kb on - strand, within PP_4872at 5539.104 kb on - strand, within PP_4872at 5539.104 kb on - strand, within PP_4872at 5539.136 kb on + strand, within PP_4872at 5539.136 kb on + strand, within PP_4872at 5539.136 kb on + strand, within PP_4872at 5539.137 kb on - strand, within PP_4872at 5539.147 kb on + strand, within PP_4872at 5539.148 kb on - strand, within PP_4872at 5539.238 kb on + strandat 5539.238 kb on + strand, within PP_4872at 5539.250 kb on + strand, within PP_4872at 5539.251 kb on - strand, within PP_4872at 5539.307 kb on + strand, within PP_4872at 5539.308 kb on - strand, within PP_4872at 5539.416 kb on - strand, within PP_4872at 5539.416 kb on - strand, within PP_4872

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
remove
5,537,024 + -1.1
5,537,024 + -0.8
5,537,025 - -0.8
5,537,025 - +0.1
5,537,025 - -0.8
5,537,025 - -0.8
5,537,025 - -0.1
5,537,057 + -0.3
5,537,127 + +0.8
5,537,127 + +0.2
5,537,191 - PP_4870 0.14 +0.1
5,537,191 - PP_4870 0.14 +0.0
5,537,301 + PP_4870 0.39 -0.2
5,537,302 - PP_4870 0.39 +1.3
5,537,472 + PP_4870 0.77 +0.5
5,537,473 - PP_4870 0.77 -0.6
5,537,558 + -0.0
5,537,599 + +1.4
5,537,599 + +0.9
5,537,642 + +0.9
5,537,643 - +0.7
5,537,697 + +1.6
5,537,697 + -1.0
5,537,765 + -1.3
5,537,765 + +1.3
5,537,795 - -0.8
5,537,931 - -0.4
5,537,981 - -1.1
5,538,040 + PP_4871 0.20 +0.5
5,538,041 - PP_4871 0.20 +2.5
5,538,041 - PP_4871 0.20 +2.5
5,538,256 - PP_4871 0.57 +1.8
5,538,343 + PP_4871 0.71 +1.7
5,538,372 + PP_4871 0.76 +1.1
5,538,372 + PP_4871 0.76 -0.4
5,538,483 + +2.5
5,538,484 - -0.6
5,538,620 + -1.6
5,538,621 - -0.8
5,538,621 - -0.7
5,538,632 + -0.3
5,538,878 + PP_4872 0.10 +0.7
5,538,878 + PP_4872 0.10 -0.8
5,538,879 - PP_4872 0.11 -0.3
5,538,879 - PP_4872 0.11 -2.2
5,538,879 - PP_4872 0.11 +0.8
5,538,882 + PP_4872 0.11 +0.7
5,538,883 - PP_4872 0.11 +0.4
5,538,883 - PP_4872 0.11 +3.1
5,538,883 - PP_4872 0.11 +1.9
5,538,883 - PP_4872 0.11 -0.3
5,538,883 - PP_4872 0.11 +0.6
5,539,067 + PP_4872 0.19 +0.2
5,539,068 - PP_4872 0.19 -0.8
5,539,103 + PP_4872 0.20 +0.4
5,539,103 + PP_4872 0.20 +0.6
5,539,103 + PP_4872 0.20 -0.9
5,539,103 + PP_4872 0.20 +0.3
5,539,103 + PP_4872 0.20 +0.1
5,539,104 - PP_4872 0.20 +0.0
5,539,104 - PP_4872 0.20 -0.7
5,539,104 - PP_4872 0.20 +2.5
5,539,136 + PP_4872 0.21 +0.8
5,539,136 + PP_4872 0.21 +0.1
5,539,136 + PP_4872 0.21 +0.6
5,539,137 - PP_4872 0.21 -0.1
5,539,147 + PP_4872 0.22 -0.8
5,539,148 - PP_4872 0.22 -2.0
5,539,238 + +2.5
5,539,238 + PP_4872 0.26 +0.4
5,539,250 + PP_4872 0.26 -0.8
5,539,251 - PP_4872 0.26 -0.5
5,539,307 + PP_4872 0.29 -0.1
5,539,308 - PP_4872 0.29 +0.7
5,539,416 - PP_4872 0.33 -0.1
5,539,416 - PP_4872 0.33 -2.1

Or see this region's nucleotide sequence