Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4829 and PP_4830 are separated by 243 nucleotides PP_4830 and PP_4831 overlap by 8 nucleotides
PP_4829: PP_4829 - putative precorrin-3B synthase, at 5,495,353 to 5,496,684
_4829
PP_4830: PP_4830 - precorrin-6Y C(5,15)-methyltransferase, at 5,496,928 to 5,498,139
_4830
PP_4831: PP_4831 - putative cobalt-precorrin-6A synthase, at 5,498,132 to 5,499,226
_4831
Position (kb) 5496
5497
5498
5499 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5495.996 kb on + strand, within PP_4829 at 5495.996 kb on + strand, within PP_4829 at 5496.583 kb on - strand at 5496.583 kb on - strand at 5496.707 kb on + strand at 5496.708 kb on - strand at 5496.708 kb on - strand at 5496.722 kb on + strand at 5496.722 kb on + strand at 5496.722 kb on + strand at 5496.723 kb on - strand at 5496.723 kb on - strand at 5496.723 kb on - strand at 5496.723 kb on - strand at 5496.781 kb on + strand at 5496.781 kb on + strand at 5496.861 kb on + strand at 5496.862 kb on - strand at 5496.868 kb on - strand at 5496.926 kb on - strand at 5496.926 kb on - strand at 5496.947 kb on + strand at 5496.947 kb on + strand at 5496.947 kb on + strand at 5496.948 kb on - strand at 5496.948 kb on - strand at 5496.950 kb on + strand at 5496.950 kb on + strand at 5496.951 kb on - strand at 5496.951 kb on - strand at 5496.951 kb on - strand at 5497.160 kb on + strand, within PP_4830 at 5497.161 kb on + strand, within PP_4830 at 5497.161 kb on + strand, within PP_4830 at 5497.161 kb on + strand, within PP_4830 at 5497.162 kb on - strand, within PP_4830 at 5497.162 kb on - strand, within PP_4830 at 5497.162 kb on - strand, within PP_4830 at 5497.162 kb on - strand, within PP_4830 at 5497.169 kb on - strand, within PP_4830 at 5497.549 kb on - strand, within PP_4830 at 5497.601 kb on - strand, within PP_4830 at 5497.817 kb on + strand, within PP_4830 at 5497.919 kb on + strand, within PP_4830 at 5497.919 kb on + strand, within PP_4830 at 5497.919 kb on + strand, within PP_4830 at 5497.919 kb on + strand, within PP_4830 at 5498.118 kb on - strand at 5498.119 kb on - strand at 5498.179 kb on + strand at 5498.179 kb on + strand at 5498.180 kb on - strand at 5498.180 kb on - strand at 5498.390 kb on + strand, within PP_4831 at 5498.390 kb on + strand, within PP_4831 at 5498.390 kb on + strand, within PP_4831 at 5498.390 kb on + strand, within PP_4831 at 5498.391 kb on - strand, within PP_4831 at 5498.392 kb on + strand, within PP_4831 at 5498.705 kb on + strand, within PP_4831 at 5498.706 kb on - strand, within PP_4831 at 5498.706 kb on - strand, within PP_4831 at 5498.753 kb on + strand, within PP_4831 at 5498.754 kb on - strand, within PP_4831 at 5498.763 kb on + strand, within PP_4831 at 5498.764 kb on - strand, within PP_4831 at 5498.764 kb on - strand, within PP_4831 at 5498.811 kb on - strand, within PP_4831
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 5,495,996 + PP_4829 0.48 -0.9 5,495,996 + PP_4829 0.48 -1.5 5,496,583 - -0.4 5,496,583 - +0.6 5,496,707 + +0.5 5,496,708 - -1.0 5,496,708 - -0.6 5,496,722 + +2.2 5,496,722 + +0.5 5,496,722 + -0.3 5,496,723 - +1.0 5,496,723 - -0.9 5,496,723 - +0.8 5,496,723 - +0.3 5,496,781 + +2.5 5,496,781 + -0.0 5,496,861 + +0.3 5,496,862 - +0.5 5,496,868 - +0.2 5,496,926 - -1.4 5,496,926 - -1.3 5,496,947 + -0.9 5,496,947 + +1.5 5,496,947 + -0.5 5,496,948 - -1.4 5,496,948 - -1.0 5,496,950 + +0.0 5,496,950 + +0.2 5,496,951 - -1.5 5,496,951 - -2.3 5,496,951 - +0.8 5,497,160 + PP_4830 0.19 +1.5 5,497,161 + PP_4830 0.19 -1.1 5,497,161 + PP_4830 0.19 -1.5 5,497,161 + PP_4830 0.19 -0.9 5,497,162 - PP_4830 0.19 +1.5 5,497,162 - PP_4830 0.19 -1.3 5,497,162 - PP_4830 0.19 +0.9 5,497,162 - PP_4830 0.19 +0.5 5,497,169 - PP_4830 0.20 -1.8 5,497,549 - PP_4830 0.51 +1.0 5,497,601 - PP_4830 0.56 -0.6 5,497,817 + PP_4830 0.73 -0.4 5,497,919 + PP_4830 0.82 -1.0 5,497,919 + PP_4830 0.82 -0.6 5,497,919 + PP_4830 0.82 +0.2 5,497,919 + PP_4830 0.82 -1.9 5,498,118 - +2.5 5,498,119 - -0.8 5,498,179 + -1.5 5,498,179 + +0.2 5,498,180 - +1.0 5,498,180 - +2.5 5,498,390 + PP_4831 0.24 -1.2 5,498,390 + PP_4831 0.24 +1.2 5,498,390 + PP_4831 0.24 -0.3 5,498,390 + PP_4831 0.24 -1.1 5,498,391 - PP_4831 0.24 +0.0 5,498,392 + PP_4831 0.24 +0.5 5,498,705 + PP_4831 0.52 +0.4 5,498,706 - PP_4831 0.52 +0.3 5,498,706 - PP_4831 0.52 -0.3 5,498,753 + PP_4831 0.57 +0.7 5,498,754 - PP_4831 0.57 +2.5 5,498,763 + PP_4831 0.58 -1.0 5,498,764 - PP_4831 0.58 -0.3 5,498,764 - PP_4831 0.58 -0.7 5,498,811 - PP_4831 0.62 +0.1
Or see this region's nucleotide sequence