Strain Fitness in Pseudomonas putida KT2440 around PP_4650

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4648 and PP_4649 are separated by 105 nucleotidesPP_4649 and PP_4650 are separated by 9 nucleotidesPP_4650 and PP_4651 are separated by 3 nucleotides PP_4648: PP_4648 - Ribosomal RNA large subunit methyltransferase G, at 5,272,854 to 5,273,978 _4648 PP_4649: PP_4649 - conserved protein of unknown function, at 5,274,084 to 5,274,239 _4649 PP_4650: PP_4650 - Ubiquinol oxidase subunit II, cyanide insensitive, at 5,274,249 to 5,275,256 _4650 PP_4651: PP_4651 - Ubiquinol oxidase subunit I, cyanide insensitive, at 5,275,260 to 5,276,696 _4651 Position (kb) 5274 5275 5276Strain fitness (log2 ratio) -2 -1 0 1 2at 5273.331 kb on + strand, within PP_4648at 5273.331 kb on + strand, within PP_4648at 5273.332 kb on - strand, within PP_4648at 5273.344 kb on + strand, within PP_4648at 5273.433 kb on + strand, within PP_4648at 5273.530 kb on + strand, within PP_4648at 5273.531 kb on - strand, within PP_4648at 5273.625 kb on + strand, within PP_4648at 5273.626 kb on - strand, within PP_4648at 5273.626 kb on - strand, within PP_4648at 5273.646 kb on + strand, within PP_4648at 5273.646 kb on + strand, within PP_4648at 5273.646 kb on + strand, within PP_4648at 5273.647 kb on - strand, within PP_4648at 5273.795 kb on - strand, within PP_4648at 5273.795 kb on - strand, within PP_4648at 5273.797 kb on + strandat 5273.797 kb on + strand, within PP_4648at 5273.851 kb on + strand, within PP_4648at 5273.852 kb on - strand, within PP_4648at 5273.852 kb on - strand, within PP_4648at 5273.865 kb on + strand, within PP_4648at 5273.865 kb on + strand, within PP_4648at 5273.865 kb on + strand, within PP_4648at 5273.865 kb on + strand, within PP_4648at 5273.866 kb on - strandat 5273.872 kb on + strandat 5273.873 kb on - strandat 5273.873 kb on - strandat 5273.873 kb on - strandat 5273.976 kb on + strandat 5273.976 kb on + strandat 5274.041 kb on - strandat 5274.041 kb on - strandat 5274.041 kb on - strandat 5274.082 kb on + strandat 5274.082 kb on + strandat 5274.082 kb on + strandat 5274.083 kb on - strandat 5274.250 kb on + strandat 5274.250 kb on + strandat 5274.251 kb on - strandat 5274.292 kb on + strandat 5274.292 kb on + strandat 5274.292 kb on + strandat 5274.292 kb on + strandat 5274.293 kb on - strandat 5274.293 kb on - strandat 5274.293 kb on - strandat 5274.293 kb on - strandat 5274.293 kb on - strandat 5274.295 kb on + strandat 5274.295 kb on + strandat 5274.295 kb on + strandat 5274.296 kb on - strandat 5274.309 kb on - strandat 5274.309 kb on - strandat 5274.310 kb on + strandat 5274.310 kb on + strandat 5274.310 kb on + strandat 5274.310 kb on + strandat 5274.310 kb on + strandat 5274.311 kb on - strandat 5274.311 kb on - strandat 5274.311 kb on - strandat 5274.311 kb on - strandat 5274.320 kb on + strandat 5274.342 kb on - strandat 5274.416 kb on + strand, within PP_4650at 5274.417 kb on - strand, within PP_4650at 5274.417 kb on - strand, within PP_4650at 5274.445 kb on + strand, within PP_4650at 5274.446 kb on - strand, within PP_4650at 5274.462 kb on + strand, within PP_4650at 5274.463 kb on - strand, within PP_4650at 5274.487 kb on + strand, within PP_4650at 5274.523 kb on + strand, within PP_4650at 5274.524 kb on - strand, within PP_4650at 5274.746 kb on - strand, within PP_4650at 5274.746 kb on - strand, within PP_4650at 5274.899 kb on + strand, within PP_4650at 5274.900 kb on - strand, within PP_4650at 5275.006 kb on + strand, within PP_4650at 5275.007 kb on - strand, within PP_4650at 5275.030 kb on + strand, within PP_4650at 5275.031 kb on - strand, within PP_4650at 5275.198 kb on + strandat 5275.198 kb on + strandat 5275.198 kb on + strandat 5275.198 kb on + strandat 5275.198 kb on + strandat 5275.199 kb on - strandat 5275.199 kb on - strandat 5275.199 kb on - strandat 5275.199 kb on - strandat 5275.414 kb on + strand, within PP_4651at 5275.414 kb on + strand, within PP_4651at 5275.414 kb on + strand, within PP_4651at 5275.415 kb on - strand, within PP_4651at 5275.445 kb on - strand, within PP_4651at 5275.445 kb on - strand, within PP_4651at 5275.445 kb on - strand, within PP_4651at 5275.486 kb on + strand, within PP_4651at 5275.486 kb on + strand, within PP_4651at 5275.486 kb on + strand, within PP_4651at 5275.486 kb on + strand, within PP_4651at 5275.487 kb on - strand, within PP_4651at 5275.487 kb on - strand, within PP_4651at 5275.487 kb on - strand, within PP_4651at 5275.528 kb on + strand, within PP_4651at 5275.528 kb on + strand, within PP_4651at 5275.529 kb on - strand, within PP_4651at 5275.529 kb on - strand, within PP_4651at 5275.639 kb on + strand, within PP_4651at 5275.640 kb on - strand, within PP_4651at 5275.640 kb on - strand, within PP_4651at 5275.745 kb on + strand, within PP_4651at 5275.850 kb on + strand, within PP_4651at 5275.851 kb on - strand, within PP_4651at 5275.965 kb on - strand, within PP_4651

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Per-strain Table

Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
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5,273,331 + PP_4648 0.42 -1.0
5,273,331 + PP_4648 0.42 +0.5
5,273,332 - PP_4648 0.42 -0.3
5,273,344 + PP_4648 0.44 +0.5
5,273,433 + PP_4648 0.51 -1.4
5,273,530 + PP_4648 0.60 -0.5
5,273,531 - PP_4648 0.60 +0.1
5,273,625 + PP_4648 0.69 +1.5
5,273,626 - PP_4648 0.69 -0.2
5,273,626 - PP_4648 0.69 +1.1
5,273,646 + PP_4648 0.70 +0.1
5,273,646 + PP_4648 0.70 -0.6
5,273,646 + PP_4648 0.70 -0.9
5,273,647 - PP_4648 0.70 +0.7
5,273,795 - PP_4648 0.84 -0.3
5,273,795 - PP_4648 0.84 -1.0
5,273,797 + +2.5
5,273,797 + PP_4648 0.84 -0.0
5,273,851 + PP_4648 0.89 -1.2
5,273,852 - PP_4648 0.89 -0.9
5,273,852 - PP_4648 0.89 -0.2
5,273,865 + PP_4648 0.90 -0.6
5,273,865 + PP_4648 0.90 +0.5
5,273,865 + PP_4648 0.90 +0.7
5,273,865 + PP_4648 0.90 -0.7
5,273,866 - -0.8
5,273,872 + +0.1
5,273,873 - +1.1
5,273,873 - +0.5
5,273,873 - +0.1
5,273,976 + +0.8
5,273,976 + +0.5
5,274,041 - +2.5
5,274,041 - -1.1
5,274,041 - -0.3
5,274,082 + -0.6
5,274,082 + -0.5
5,274,082 + -0.4
5,274,083 - -0.0
5,274,250 + +1.0
5,274,250 + -0.0
5,274,251 - -0.0
5,274,292 + +0.1
5,274,292 + -0.9
5,274,292 + -0.0
5,274,292 + -2.5
5,274,293 - +0.2
5,274,293 - -0.8
5,274,293 - -2.0
5,274,293 - +1.3
5,274,293 - -1.3
5,274,295 + +0.5
5,274,295 + -2.3
5,274,295 + +1.1
5,274,296 - -0.8
5,274,309 - +0.4
5,274,309 - -0.7
5,274,310 + -2.0
5,274,310 + -0.3
5,274,310 + +1.0
5,274,310 + -0.5
5,274,310 + -1.3
5,274,311 - -2.0
5,274,311 - -1.0
5,274,311 - +0.2
5,274,311 - -0.0
5,274,320 + +1.0
5,274,342 - +2.0
5,274,416 + PP_4650 0.17 -0.5
5,274,417 - PP_4650 0.17 -2.3
5,274,417 - PP_4650 0.17 -0.2
5,274,445 + PP_4650 0.19 -2.0
5,274,446 - PP_4650 0.20 -0.9
5,274,462 + PP_4650 0.21 -1.4
5,274,463 - PP_4650 0.21 -0.8
5,274,487 + PP_4650 0.24 -1.3
5,274,523 + PP_4650 0.27 -1.8
5,274,524 - PP_4650 0.27 -1.7
5,274,746 - PP_4650 0.49 -1.7
5,274,746 - PP_4650 0.49 -0.6
5,274,899 + PP_4650 0.64 -0.0
5,274,900 - PP_4650 0.65 +0.8
5,275,006 + PP_4650 0.75 +0.1
5,275,007 - PP_4650 0.75 -0.5
5,275,030 + PP_4650 0.77 -1.2
5,275,031 - PP_4650 0.78 -1.9
5,275,198 + +1.5
5,275,198 + +0.2
5,275,198 + -0.9
5,275,198 + -0.1
5,275,198 + +2.5
5,275,199 - -1.3
5,275,199 - +0.3
5,275,199 - +0.8
5,275,199 - -0.5
5,275,414 + PP_4651 0.11 -0.9
5,275,414 + PP_4651 0.11 -0.6
5,275,414 + PP_4651 0.11 +1.1
5,275,415 - PP_4651 0.11 -0.5
5,275,445 - PP_4651 0.13 -0.2
5,275,445 - PP_4651 0.13 -2.2
5,275,445 - PP_4651 0.13 -0.4
5,275,486 + PP_4651 0.16 -0.1
5,275,486 + PP_4651 0.16 -1.9
5,275,486 + PP_4651 0.16 +1.1
5,275,486 + PP_4651 0.16 +0.6
5,275,487 - PP_4651 0.16 +0.5
5,275,487 - PP_4651 0.16 +0.4
5,275,487 - PP_4651 0.16 -0.3
5,275,528 + PP_4651 0.19 +0.2
5,275,528 + PP_4651 0.19 -0.9
5,275,529 - PP_4651 0.19 -1.1
5,275,529 - PP_4651 0.19 -0.6
5,275,639 + PP_4651 0.26 -0.7
5,275,640 - PP_4651 0.26 -0.5
5,275,640 - PP_4651 0.26 -0.2
5,275,745 + PP_4651 0.34 -0.3
5,275,850 + PP_4651 0.41 +0.3
5,275,851 - PP_4651 0.41 -0.5
5,275,965 - PP_4651 0.49 +0.7

Or see this region's nucleotide sequence