Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4613 and PP_5700 are separated by 178 nucleotides PP_5700 and PP_4614 are separated by 23 nucleotides PP_4614 and PP_4615 are separated by 3 nucleotides PP_4615 and PP_5701 are separated by 87 nucleotides PP_5701 and PP_4616 are separated by 280 nucleotides PP_4616 and PP_4617 are separated by 58 nucleotides
PP_4613: PP_4613 - outer membrane ferric citrate porin, at 5,234,667 to 5,236,991
_4613
PP_5700: PP_5700 - conserved protein of unknown function, at 5,237,170 to 5,237,517
_5700
PP_4614: PP_4614 - conserved protein of unknown function, at 5,237,541 to 5,237,927
_4614
PP_4615: PP_4615 - conserved membrane protein of unknown function, at 5,237,931 to 5,238,401
_4615
PP_5701: PP_5701 - conserved protein of unknown function, at 5,238,489 to 5,238,692
_5701
PP_4616: PP_4616 - conserved protein of unknown function, at 5,238,973 to 5,239,236
_4616
PP_4617: PP_4617 - leucine dehydrogenase, at 5,239,295 to 5,240,314
_4617
Position (kb) 5237
5238
5239 Strain fitness (log2 ratio) -1
0
1
2 at 5236.941 kb on + strand at 5236.941 kb on + strand at 5236.941 kb on + strand at 5236.942 kb on - strand at 5236.942 kb on - strand at 5236.958 kb on + strand at 5236.958 kb on + strand at 5236.958 kb on + strand at 5236.958 kb on + strand at 5236.959 kb on - strand at 5236.959 kb on - strand at 5236.959 kb on - strand at 5236.965 kb on + strand at 5236.966 kb on - strand at 5237.017 kb on + strand at 5237.018 kb on - strand at 5237.088 kb on - strand at 5237.932 kb on + strand at 5237.932 kb on + strand at 5237.933 kb on - strand at 5237.992 kb on + strand, within PP_4615 at 5238.001 kb on + strand, within PP_4615 at 5238.001 kb on + strand, within PP_4615 at 5238.052 kb on + strand, within PP_4615 at 5238.052 kb on + strand, within PP_4615 at 5238.130 kb on + strand, within PP_4615 at 5238.130 kb on + strand, within PP_4615 at 5238.130 kb on + strand, within PP_4615 at 5238.130 kb on + strand, within PP_4615 at 5238.131 kb on - strand, within PP_4615 at 5238.169 kb on + strand, within PP_4615 at 5238.170 kb on - strand, within PP_4615 at 5238.170 kb on - strand, within PP_4615 at 5238.170 kb on - strand, within PP_4615 at 5238.170 kb on - strand, within PP_4615 at 5238.199 kb on + strand, within PP_4615 at 5238.199 kb on + strand, within PP_4615 at 5238.200 kb on - strand, within PP_4615 at 5238.314 kb on - strand, within PP_4615 at 5238.314 kb on - strand, within PP_4615 at 5238.419 kb on + strand at 5238.427 kb on + strand at 5238.427 kb on + strand at 5238.469 kb on + strand at 5238.619 kb on + strand, within PP_5701 at 5238.620 kb on - strand, within PP_5701 at 5238.620 kb on - strand, within PP_5701 at 5238.691 kb on - strand at 5238.691 kb on - strand at 5238.708 kb on + strand at 5238.709 kb on - strand at 5238.709 kb on - strand at 5238.709 kb on - strand at 5238.709 kb on - strand at 5238.709 kb on - strand at 5238.758 kb on - strand at 5238.813 kb on + strand at 5238.814 kb on - strand at 5238.814 kb on - strand at 5238.814 kb on - strand at 5238.923 kb on + strand at 5238.961 kb on + strand at 5238.961 kb on + strand at 5238.961 kb on + strand at 5238.962 kb on - strand at 5238.962 kb on - strand at 5238.992 kb on - strand at 5238.992 kb on - strand at 5239.029 kb on + strand, within PP_4616 at 5239.144 kb on + strand, within PP_4616 at 5239.144 kb on + strand, within PP_4616 at 5239.144 kb on + strand, within PP_4616 at 5239.145 kb on - strand, within PP_4616 at 5239.145 kb on - strand, within PP_4616 at 5239.145 kb on - strand, within PP_4616 at 5239.145 kb on - strand, within PP_4616 at 5239.145 kb on - strand, within PP_4616 at 5239.238 kb on - strand at 5239.259 kb on + strand at 5239.260 kb on - strand at 5239.302 kb on + strand at 5239.302 kb on + strand at 5239.303 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 5,236,941 + -1.0 5,236,941 + -0.6 5,236,941 + -0.7 5,236,942 - +1.1 5,236,942 - +0.0 5,236,958 + +1.2 5,236,958 + -0.5 5,236,958 + -0.2 5,236,958 + -0.5 5,236,959 - +0.8 5,236,959 - +0.3 5,236,959 - +0.3 5,236,965 + +0.2 5,236,966 - -0.8 5,237,017 + +2.5 5,237,018 - +1.8 5,237,088 - -0.7 5,237,932 + +0.8 5,237,932 + +1.5 5,237,933 - -0.0 5,237,992 + PP_4615 0.13 -1.1 5,238,001 + PP_4615 0.15 -0.8 5,238,001 + PP_4615 0.15 -0.3 5,238,052 + PP_4615 0.26 +0.3 5,238,052 + PP_4615 0.26 +0.0 5,238,130 + PP_4615 0.42 -0.1 5,238,130 + PP_4615 0.42 +1.0 5,238,130 + PP_4615 0.42 -1.4 5,238,130 + PP_4615 0.42 +0.2 5,238,131 - PP_4615 0.42 +0.3 5,238,169 + PP_4615 0.51 +2.5 5,238,170 - PP_4615 0.51 -1.1 5,238,170 - PP_4615 0.51 +1.7 5,238,170 - PP_4615 0.51 -0.5 5,238,170 - PP_4615 0.51 +0.8 5,238,199 + PP_4615 0.57 +0.5 5,238,199 + PP_4615 0.57 -0.2 5,238,200 - PP_4615 0.57 -0.2 5,238,314 - PP_4615 0.81 -0.1 5,238,314 - PP_4615 0.81 -0.4 5,238,419 + -0.2 5,238,427 + +1.2 5,238,427 + +0.5 5,238,469 + -0.0 5,238,619 + PP_5701 0.64 +0.2 5,238,620 - PP_5701 0.64 +1.2 5,238,620 - PP_5701 0.64 -0.3 5,238,691 - +0.8 5,238,691 - +0.3 5,238,708 + +1.0 5,238,709 - +0.8 5,238,709 - +2.3 5,238,709 - -0.4 5,238,709 - +0.8 5,238,709 - +0.9 5,238,758 - +0.5 5,238,813 + +0.2 5,238,814 - +1.0 5,238,814 - -0.5 5,238,814 - +2.5 5,238,923 + +0.6 5,238,961 + -0.1 5,238,961 + +1.0 5,238,961 + +0.4 5,238,962 - -0.3 5,238,962 - -0.5 5,238,992 - -0.5 5,238,992 - +1.0 5,239,029 + PP_4616 0.21 +0.2 5,239,144 + PP_4616 0.65 +1.4 5,239,144 + PP_4616 0.65 +1.0 5,239,144 + PP_4616 0.65 +0.3 5,239,145 - PP_4616 0.65 +0.1 5,239,145 - PP_4616 0.65 +0.2 5,239,145 - PP_4616 0.65 +0.1 5,239,145 - PP_4616 0.65 +0.3 5,239,145 - PP_4616 0.65 +0.9 5,239,238 - -0.3 5,239,259 + -0.1 5,239,260 - -0.7 5,239,302 + -1.0 5,239,302 + -0.0 5,239,303 - -1.0
Or see this region's nucleotide sequence