Strain Fitness in Pseudomonas putida KT2440 around PP_2714

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
3,102,518	+		PP_2712	0.54	-0.7
3,102,539	+		PP_2712	0.57	+0.4
3,102,540	-				+1.6
3,102,540	-		PP_2712	0.58	-1.9
3,102,644	+		PP_2712	0.74	+0.5
3,102,691	+		PP_2712	0.81	-1.3
3,103,105	+		PP_2713	0.44	-0.0
3,103,106	-		PP_2713	0.44	-0.2
3,103,240	+		PP_2713	0.64	+0.2
3,103,241	-		PP_2713	0.64	-0.7
3,103,276	+		PP_2713	0.70	+0.8
3,103,277	-		PP_2713	0.70	-0.2
3,103,277	-		PP_2713	0.70	-1.6
3,103,316	+		PP_2713	0.76	-0.6
3,103,317	-		PP_2713	0.76	+0.1
3,103,317	-		PP_2713	0.76	+0.4
3,103,348	+		PP_2713	0.81	-1.0
3,103,348	+		PP_2713	0.81	+1.0
3,103,397	-		PP_2713	0.88	-0.7
3,103,397	-		PP_2713	0.88	+0.0
3,103,453	+				+0.9
3,103,550	+				-2.0
3,103,550	+				-1.4
3,103,554	+				-2.7
3,103,555	-				-2.0
3,103,555	-				-2.3
3,103,555	-				-1.5
3,103,585	+				-1.7
3,103,585	+				-2.0
3,103,585	+				-2.0
3,103,585	+				-2.1
3,103,586	-				-2.2
3,103,586	-				-2.9
3,103,586	-				-2.6
3,103,586	-				-2.6
3,103,586	-				-1.9
3,103,587	+				-2.0
3,103,587	+				+0.3
3,103,588	-				-2.0
3,103,671	+		PP_2714	0.15	-2.0
3,103,672	-		PP_2714	0.15	-0.4
3,103,784	+		PP_2714	0.23	-1.0
3,103,785	-		PP_2714	0.23	-2.3
3,103,785	-		PP_2714	0.23	-3.9
3,103,785	-		PP_2714	0.23	-1.2
3,103,785	-		PP_2714	0.23	-2.7
3,103,833	+		PP_2714	0.26	-1.0
3,103,834	-		PP_2714	0.26	-0.7
3,103,901	+		PP_2714	0.31	-1.9
3,103,901	+		PP_2714	0.31	-1.0
3,104,223	-		PP_2714	0.55	-2.5
3,104,698	-		PP_2714	0.90	-0.2
3,104,776	+				+0.1
3,104,814	-				+0.0
3,104,880	+				-0.6
3,104,880	+				+1.6
3,104,954	+		PP_2715	0.15	-0.9
3,104,995	+		PP_2715	0.21	-0.0
3,104,999	-		PP_2715	0.21	-1.7
3,105,041	-		PP_2715	0.27	+0.9
3,105,325	-		PP_2715	0.68	+0.5
3,105,414	+		PP_2715	0.80	+0.3
3,105,414	+		PP_2715	0.80	+0.5
3,105,454	+		PP_2715	0.86	+0.6
3,105,697	-		PP_2716	0.28	-0.4
3,105,697	-		PP_2716	0.28	-0.9