Strain Fitness in Pseudomonas putida KT2440 around PP_2659

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
3,045,336	-				-0.3
3,045,348	+				+0.2
3,045,402	-				-0.4
3,045,402	-				-0.1
3,045,416	-				-0.2
3,045,503	+				-0.5
3,045,565	+		PP_2658	0.16	-0.6
3,045,566	-		PP_2658	0.16	+0.0
3,045,663	+		PP_2658	0.27	+0.5
3,046,126	+		PP_2658	0.79	-0.4
3,046,126	+		PP_2658	0.79	+0.1
3,046,193	-		PP_2658	0.86	+0.7
3,046,260	-				-1.2
3,046,260	-				-0.2
3,046,260	-				+0.4
3,046,312	+				-0.0
3,046,312	+				+0.0
3,046,313	-				+0.0
3,046,342	+				-0.6
3,046,342	+				-0.0
3,046,419	+		PP_2659	0.12	-0.5
3,046,420	-		PP_2659	0.13	-0.9
3,046,424	+		PP_2659	0.13	-2.0
3,046,536	-		PP_2659	0.26	-2.1
3,046,536	-		PP_2659	0.26	+0.2
3,046,599	+				+1.0
3,046,624	+		PP_2659	0.37	-0.3
3,046,624	+		PP_2659	0.37	-0.5
3,046,625	-		PP_2659	0.37	+0.5
3,046,635	+		PP_2659	0.38	-1.8
3,046,635	+		PP_2659	0.38	-1.8
3,046,636	-		PP_2659	0.38	-0.6
3,046,636	-		PP_2659	0.38	+0.1
3,046,962	+		PP_2659	0.77	-1.0
3,046,963	-		PP_2659	0.77	-0.5
3,046,963	-		PP_2659	0.77	-0.4
3,046,986	-		PP_2659	0.80	-2.5
3,047,040	-		PP_2659	0.86	-0.7
3,047,065	-		PP_2659	0.89	-1.9
3,047,215	+				+0.5
3,047,225	+				-0.5
3,047,243	+				-0.9
3,047,244	-				-1.6
3,047,393	-		PP_2660	0.21	+0.0
3,047,393	-		PP_2660	0.21	+0.4
3,047,410	+		PP_2660	0.23	-0.2
3,047,410	+		PP_2660	0.23	+0.0
3,047,410	+		PP_2660	0.23	-0.1
3,047,411	-		PP_2660	0.23	+0.1
3,047,484	-		PP_2660	0.34	+0.1
3,047,484	-		PP_2660	0.34	+0.8
3,047,484	-		PP_2660	0.34	+0.1
3,047,584	+		PP_2660	0.48	-0.3
3,047,584	+		PP_2660	0.48	-0.7
3,047,593	+		PP_2660	0.49	-0.8
3,047,641	+		PP_2660	0.56	-1.3
3,047,641	+				+0.3
3,047,641	+		PP_2660	0.56	+0.7
3,047,641	+		PP_2660	0.56	+0.2
3,047,642	-		PP_2660	0.56	+0.4
3,047,642	-		PP_2660	0.56	-0.2
3,047,642	-		PP_2660	0.56	-0.7
3,047,642	-		PP_2660	0.56	+1.2
3,047,642	-		PP_2660	0.56	+0.3
3,047,642	-		PP_2660	0.56	+0.2
3,047,645	+		PP_2660	0.57	-0.6
3,047,645	+		PP_2660	0.57	+0.3
3,047,645	+		PP_2660	0.57	+0.8
3,047,645	+		PP_2660	0.57	-1.1
3,047,646	-		PP_2660	0.57	-1.1
3,047,703	+		PP_2660	0.65	-0.9
3,047,704	-		PP_2660	0.65	+0.7
3,047,704	-		PP_2660	0.65	-0.3
3,047,714	-		PP_2660	0.67	-1.0
3,047,773	+		PP_2660	0.75	-0.5
3,047,795	+		PP_2660	0.78	+0.7
3,047,796	-		PP_2660	0.78	+0.0
3,047,830	+		PP_2660	0.83	+0.4