Strain Fitness in Pseudomonas putida KT2440 around PP_2554

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
2,900,110	-		PP_2553	0.62	+2.6
2,900,126	+		PP_2553	0.63	-1.0
2,900,126	+		PP_2553	0.63	+0.6
2,900,181	+		PP_2553	0.67	-0.7
2,900,182	-		PP_2553	0.67	-0.8
2,900,182	-		PP_2553	0.67	+0.1
2,900,184	+		PP_2553	0.68	-0.2
2,900,185	-		PP_2553	0.68	+0.4
2,900,185	-		PP_2553	0.68	+0.9
2,900,316	+		PP_2553	0.77	-0.2
2,900,316	+		PP_2553	0.77	+0.1
2,900,316	+		PP_2553	0.77	+0.4
2,900,317	-		PP_2553	0.77	-0.5
2,900,417	+		PP_2553	0.84	+1.0
2,900,445	+		PP_2553	0.87	-0.2
2,900,446	-		PP_2553	0.87	-1.5
2,900,457	+		PP_2553	0.87	+1.0
2,900,457	+		PP_2553	0.87	+0.4
2,900,457	+		PP_2553	0.87	-0.2
2,900,458	-		PP_2553	0.87	-0.6
2,900,458	-		PP_2553	0.87	-0.3
2,900,458	-		PP_2553	0.87	-1.4
2,900,458	-		PP_2553	0.87	+1.2
2,900,544	+				-0.2
2,900,545	-				+0.3
2,900,560	-				-0.2
2,900,579	+				+0.6
2,900,579	+				+0.6
2,900,580	-				+0.3
2,900,580	-				-0.0
2,900,580	-				+2.3
2,900,645	+				+2.6
2,900,645	+				-0.2
2,900,646	-				-0.7
2,900,646	-				+0.6
2,900,646	-				-0.6
2,900,646	-				+0.6
2,900,677	-				-0.1
2,900,746	+				-0.9
2,900,746	+				+0.0
2,900,747	-				+1.0
2,900,747	-				-0.0
2,900,747	-				-0.2
2,900,828	+				-1.9
2,900,891	-				+2.0
2,901,030	+				-1.2
2,901,031	-				-0.4
2,901,033	+				+0.4
2,901,034	-				-1.4
2,901,316	-		PP_2554	0.21	-1.7
2,901,352	-		PP_2554	0.23	+0.2
2,901,796	-		PP_2554	0.46	-1.3
2,901,933	-		PP_2554	0.53	-1.3
2,901,933	-		PP_2554	0.53	+0.3
2,901,961	-		PP_2554	0.55	+0.1
2,902,146	+		PP_2554	0.64	+0.5
2,902,146	+		PP_2554	0.64	+0.6
2,902,146	+		PP_2554	0.64	-0.1
2,902,147	-		PP_2554	0.64	+1.8
2,902,147	-		PP_2554	0.64	+0.4
2,902,296	+		PP_2554	0.72	-0.5
2,902,296	+		PP_2554	0.72	-0.8
2,902,297	-		PP_2554	0.72	-1.5
2,902,297	-		PP_2554	0.72	-0.9
2,902,566	+		PP_2554	0.86	-0.9
2,902,566	+				+1.6
2,902,569	+		PP_2554	0.86	-1.2
2,902,569	+		PP_2554	0.86	-0.9
2,902,570	-		PP_2554	0.87	-0.9
2,902,570	-		PP_2554	0.87	-1.3
2,902,570	-		PP_2554	0.87	+0.6
2,902,626	+		PP_2554	0.89	-0.6
2,902,626	+		PP_2554	0.89	-0.2
2,902,626	+		PP_2554	0.89	+0.2
2,902,629	+		PP_2554	0.90	-0.6
2,902,629	+		PP_2554	0.90	-0.4
2,902,630	-		PP_2554	0.90	-0.6
2,902,641	+				-0.7
2,902,680	+				-1.2
2,902,682	+				+0.2
2,902,682	+				-1.3
2,902,824	+				-0.3
2,902,825	-				+1.0
2,903,025	+		PP_2555	0.19	-1.5
2,903,026	-		PP_2555	0.19	-0.6
2,903,161	+		PP_2555	0.39	-0.2
2,903,162	-		PP_2555	0.39	+1.7
2,903,229	+		PP_2555	0.49	-0.5
2,903,229	+		PP_2555	0.49	-0.7
2,903,230	-		PP_2555	0.49	-0.7
2,903,271	+		PP_2555	0.55	+1.5
2,903,630	-				-0.4
2,903,645	+				+0.6