Strain Fitness in Pseudomonas putida KT2440 around PP_2520

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
2,867,624	+				-1.0
2,867,624	+				-0.4
2,867,624	+				-0.4
2,867,625	-				-0.4
2,867,625	-				-1.8
2,868,078	+				+0.6
2,868,142	+				-0.9
2,868,143	-				+0.7
2,868,143	-				+0.8
2,868,143	-				+1.0
2,868,143	-				-0.4
2,868,143	-				-0.7
2,868,143	-				+1.0
2,868,170	+				-0.2
2,868,171	-				-0.7
2,868,171	-				-0.6
2,868,171	-				+0.2
2,868,171	-				-0.4
2,868,264	+				-1.0
2,868,389	+		PP_2520	0.38	-0.9
2,868,443	+		PP_2520	0.84	-0.4
2,868,443	+		PP_2520	0.84	-0.7
2,868,444	-		PP_2520	0.85	-2.7
2,868,444	-		PP_2520	0.85	-1.8
2,868,527	+				-0.2
2,868,527	+				-0.9
2,868,527	+				+0.4
2,868,527	+				-0.4
2,868,527	+				-0.6
2,868,527	+				+0.2
2,868,574	+				+0.6
2,868,575	-				+0.5
2,868,575	-				-0.7
2,868,609	+				+1.6
2,868,610	-				-0.2
2,868,983	+				+0.7
2,869,005	+				+0.3
2,869,104	+				-1.5
2,869,251	+				+0.6
2,869,251	+				-0.1
2,869,252	-				-0.2