Strain Fitness in Pseudomonas putida KT2440 around PP_2443

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
2,789,373	+				-0.8
2,789,374	-				-1.5
2,789,374	-				-1.7
2,789,374	-				+1.0
2,789,374	-				+1.0
2,789,439	-		PP_2441	0.17	-0.5
2,789,439	-		PP_2441	0.17	-0.7
2,789,439	-		PP_2441	0.17	-0.4
2,789,766	+		PP_2442	0.26	+0.6
2,789,767	-		PP_2442	0.26	-0.8
2,790,284	-				-1.2
2,790,284	-				+1.0
2,790,284	-				-0.8
2,790,287	+				+2.6
2,790,287	+				-0.4
2,790,287	+				-0.9
2,790,288	-				-1.6
2,790,288	-				+0.4
2,790,288	-				-0.0
2,790,288	-				-1.5
2,790,288	-				-0.5
2,790,288	-				-0.1
2,790,288	-				+0.2
2,790,288	-				+0.6
2,790,300	+				-1.2
2,790,301	-				+0.7
2,790,337	+				+0.2
2,790,614	+		PP_2443	0.23	+0.1
2,790,641	+		PP_2443	0.25	-0.6
2,790,700	+		PP_2443	0.30	-2.3
2,790,700	+		PP_2443	0.30	+0.9
2,790,700	+		PP_2443	0.30	-0.5
2,790,701	-		PP_2443	0.30	-1.0
2,790,701	-		PP_2443	0.30	-2.2
2,790,701	-		PP_2443	0.30	-0.2
2,790,785	+		PP_2443	0.37	+0.0
2,790,785	+		PP_2443	0.37	-0.1
2,790,901	-		PP_2443	0.47	+0.9
2,791,008	+		PP_2443	0.55	-0.8
2,791,020	+		PP_2443	0.56	+0.3
2,791,227	+		PP_2443	0.74	-0.2
2,791,300	+		PP_2443	0.80	-0.9
2,791,300	+		PP_2443	0.80	-0.0
2,791,417	-		PP_2443	0.89	-0.9
2,791,417	-		PP_2443	0.89	-1.1
2,791,417	-		PP_2443	0.89	-2.0
2,791,417	-		PP_2443	0.89	-0.4
2,791,417	-		PP_2443	0.89	-0.4
2,791,510	-				-0.6
2,791,557	+				+0.9
2,791,585	+				-0.0
2,791,590	+				-0.7
2,791,590	+				+0.1
2,791,599	+				-0.7
2,791,600	-				+1.6
2,791,600	-				-2.9
2,791,600	-				+1.1
2,791,601	+				+0.6
2,791,601	+				-0.9
2,791,601	+				-1.0
2,791,601	+				-1.8
2,791,602	-				-0.8
2,791,602	-				-0.0
2,791,640	+				+0.3
2,791,640	+				-0.5
2,791,731	-				+0.5
2,791,805	-		PP_2444	0.18	-0.1
2,792,063	+		PP_2444	0.47	-2.3
2,792,064	-		PP_2444	0.47	+0.1
2,792,064	-		PP_2444	0.47	+0.3
2,792,105	-		PP_2444	0.51	+1.3
2,792,219	+		PP_2444	0.64	-1.0
2,792,219	+		PP_2444	0.64	+0.6
2,792,336	+		PP_2444	0.77	+0.1
2,792,336	+		PP_2444	0.77	-0.0
2,792,337	-		PP_2444	0.77	+0.3
2,792,337	-		PP_2444	0.77	-0.9
2,792,337	-		PP_2444	0.77	-0.1
2,792,408	+		PP_2444	0.85	-0.1
2,792,409	-		PP_2444	0.85	+0.4
2,792,513	+				-0.9
2,792,514	-				-1.4
2,792,514	-				-0.5
2,792,528	+				+3.6
2,792,532	+				+0.8
2,792,532	+				-0.3
2,792,532	+				-0.4
2,792,533	-				-1.0
2,792,533	-				+0.6
2,792,533	-				-0.1
2,792,533	-				-0.6